Monodisperse microsphere is a kind of new polymer material with single particle size from micron to nanometer. Because of its outstanding properties, such as tiny particle size and huge specific surface area, it can be used as micro-separator, micro-storage, micro-reactor, micro-structural units, etc., and has been widely used in the fields of storage, life science, catalyst, pharmaceutical science and so on. In recent years, it has become a research hotspot for domestic and foreign scholars. Colored monodisperse microspheres have both the monodisperse property of microspheres and the color degree of dyes or fluorescent substances, which greatly improves the contrast of microspheres and thus increases their sensitivity in detection. In this paper, the methods of preparing monodisperse microspheres and color microspheres in various literatures currently are reviewed. Focusing on several representative techniques, the ideas, benefits, and drawbacks of many sample approaches are discussed in detail, the similarities and differences of various methods are compared, and the deficiencies in this field are analyzed, and their development prospects are discussed.