Preparation and Characterization of Magnetite–Chitosan Nanoparticles and Evaluation of Their Cytotoxicity Effects on MCF7 and Fibroblast Cells

Lotfi, Sheida; Ghaderi, Fahimeh; Bahari, Ali; Mahjoub, S.

doi:10.1007/s10948-017-4094-5

Cited by 30 publications

(15 citation statements)

References 22 publications

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“…The band that corresponds only to Tween 80 is presented at 1741 cm −1 , which indicates a C=O stretching vibration. The bands that correspond only to the chitosan are the vibratory stretching mode of C-N observed at 1468 cm −1 and the bending mode of N-H at 1508 cm −1 [ 33 , 34 , 35 , 36 , 37 ].…”

Section: Resultsmentioning

confidence: 99%

“…The results obtained by measuring the viability of 3T3L1 cells after exposure with magnetite nanoparticles and M-CS/Tween 80 microspheres at concentrations of 1–10,000 µg/mL were compared by those obtained by Lotfi et al In their study, they used concentrations of 10, 25, 50, 75, and 100 µg/mL of magnetite nanoparticles coated with chitosan for 24 h in MCF7 cells and fibroblasts. They obtained 78% cell viability for bare magnetite nanoparticles and a cell viability of 80% for magnetite coated with chitosan in the MCF7 cell line [ 35 ]. Viabilities obtained by our research group at concentrations of 100 µg/mL at the first 24 h are 87% for magnetite nanoparticles and 93% for M-CS/Tween 80 microspheres.…”

Section: Resultsmentioning

confidence: 99%

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A Full Set of In Vitro Assays in Chitosan/Tween 80 Microspheres Loaded with Magnetite Nanoparticles

et al. 2021

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Microspheres have been proposed for different medical applications, such as the delivery of therapeutic proteins. The first step, before evaluating the functionality of a protein delivery system, is to evaluate their biological safety. In this work, we developed chitosan/Tween 80 microspheres loaded with magnetite nanoparticles and evaluated cell damage. The formation and physical–chemical properties of the microspheres were determined by FT-IR, Raman, thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDS), dynamic light scattering (DLS), and SEM. Cell damage was evaluated by a full set of in vitro assays using a non-cancerous cell line, human erythrocytes, and human lymphocytes. At the same time, to know if these microspheres can load proteins over their surface, bovine serum albumin (BSA) immobilization was measured. Results showed 7 nm magnetite nanoparticles loaded into chitosan/Tween 80 microspheres with average sizes of 1.431 µm. At concentrations from 1 to 100 µg/mL, there was no evidence of changes in mitochondrial metabolism, cell morphology, membrane rupture, cell cycle, nor sister chromatid exchange formation. For each microgram of microspheres 1.8 µg of BSA was immobilized. The result provides the fundamental understanding of the in vitro biological behavior, and safety, of developed microspheres. Additionally, this set of assays can be helpful for researchers to evaluate different nano and microparticles.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

A Full Set of In Vitro Assays in Chitosan/Tween 80 Microspheres Loaded with Magnetite Nanoparticles

et al. 2021

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“…bands assigned, in dash lines, to chitosan biopolymer at 3440 cm −1 (νO-H and νΝ-Η from the amine group) (1), 1630 cm −1 (δΝ-Η) (2), 1407 cm −1 (νC-Ν) (3) and in the region of 1024 cm −1 (νC-Ο-C) (4) [50][51][52][53]. In addition, Fig.…”

Section: Fourier Transform Infrared (Ftir) Spectroscopymentioning

confidence: 95%

Biocompatible superparamagnetic nanoparticles with ibuprofen as potential drug carriers

et al. 2020

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This article describes the synthesis, characterization, in vitro cytotoxic and genotoxicity evaluation of chitosan-iron oxide nanoparticles (Fe 3 O 4 -CS) as vehicles for ibuprofen (IBU) molecule. Magnetite (Fe 3 O 4 ) nanoparticles were synthesized by co-precipitation of iron salts and coated with chitosan, leading to the formation of Fe 3 O 4 -CS hybrid nanoparticles, and then IBU was adsorbed on the surface of the modified nanoparticles. The physicochemical, morphological, and magnetic properties of the nanoparticles were determined by X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and SQUID magnetic measurements. The nanoparticles have shown stability in aqueous medium presenting an average hydrodynamic size of 36 ± 9 nm for Fe 3 O 4 -CS, and 132 ± 1 nm for Fe 3 O 4 -CS-IBU. The cytotoxicity of nanoparticles using the cell viability of the blood mononuclear cell fraction and the analysis of gene expression of the repair genes hMSH2, hMSH6 and NF-kB were performed to evaluate their applicability as drug carriers. The results showed that Fe 3 O 4 -CS nanoparticles are suitable candidates as magnetic vehicles for ibuprofen in biomedical applications. Graphic abstract

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“…This leads to faster and more efficient removal and rebinding of the template molecules with improved binding kinetics, which saves time and the cost of analysis. Therefore, strategies to obtain core–shell structures have been developed in recent years to introduce a myriad of cores in addition to common core materials. , Biomass-derived FCDs are trending materials due to their biocompatibility and low toxicity for utilization in optoelectronics, fluorescent sensing, and bioimaging applications. , The photoluminescent properties of FCDs can be tuned by functionalization with organic–inorganic modifiers such as amino functionalities, poly(ethylenimine), Ag and Ni doping, and biological molecules. , Among inorganic–organic linkers, alkoxysilanes are excellent linkers to introduce desirable moieties on the surface of FCDs. , Therefore, TPA is grafted on the surface of FCDs within the 3D network of mesoporous silica via the Schiff base condensation and the sol–gel process using 3-aminopropyltriethoxysilane as a linker using modified methods. − The mesopores on the surface of FCDs provide channels for the mobilization of the analyte to interact with the tripodal pockets. This interaction leads to the quenching of the contributory fluorescent signal produced due to the FCDs and TPA.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, strategies to obtain core−shell structures have been developed in recent years to introduce a myriad of cores in addition to common core materials. 21,22 Biomass-derived FCDs are trending materials due to their biocompatibility and low toxicity for utilization in optoelectronics, fluorescent sensing, and bioimaging applications. 23,24 The photoluminescent properties of FCDs can be tuned by functionalization with organic−inorganic modifiers such as amino functionalities, poly(ethylenimine), Ag and Ni doping, and biological molecules.…”

Section: Introductionmentioning

confidence: 99%

Biomass-Derived Core–Shell Carbon Dots with Embedded Tripodal Receptors for the Selective Recognition of Mefenamic Acid in Pharmaceutical Formulations and Urine

Thakur,

Mohiuddin,

Singh

et al. 2023

ACS Appl. Bio Mater.

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A tripodal amine (TPA) with −OH, N, and S donors is synthesized to functionalize a core−shell carbon dot composite (FCDs@SiO 2 -TPA) for sensing application. The TPA is characterized by spectroscopic and spectrometric techniques, and the composite is characterized by Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), Brunauer−Emmett−Teller (BET), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy-dispersive Xray spectra (EDS) techniques. The composite has the ability to recognize mefenamic acid (MFA) selectively even in the presence of other drugs like ibuprofen sodium, acetylsalicylic acid, naproxen sodium, diclofenac sodium, and ketoprofen. It can also be used for the quantification of MFA by recording the emission quenching response of the sample at λ exc. = 350 nm and λ ems. = 460 nm (linear range = 1−8 μM and LOD = 197 nM). The density functional theory calculations and 1 H NMR titration suggest quenching of the emission signal due to photoinduced electron transfer via hydrogen bonding between the probe and MFA. The composite FCDs@ SiO 2 -TPA has been demonstrated as a reliable and cost-effective sensing probe for the detection of MFA in pharmaceutical formulations, water samples, and cow urine samples.

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Preparation and Characterization of Magnetite–Chitosan Nanoparticles and Evaluation of Their Cytotoxicity Effects on MCF7 and Fibroblast Cells

Cited by 30 publications

References 22 publications

A Full Set of In Vitro Assays in Chitosan/Tween 80 Microspheres Loaded with Magnetite Nanoparticles

A Full Set of In Vitro Assays in Chitosan/Tween 80 Microspheres Loaded with Magnetite Nanoparticles

Biocompatible superparamagnetic nanoparticles with ibuprofen as potential drug carriers

Biomass-Derived Core–Shell Carbon Dots with Embedded Tripodal Receptors for the Selective Recognition of Mefenamic Acid in Pharmaceutical Formulations and Urine

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