Preparation and characterization of malonic acid cross-linked chitosan and collagen 3D scaffolds: an approach on non-covalent interactions

Mitra, Tapas; Sailakshmi, G.; Gnanamani, A.; Mandal, Asit Baran

doi:10.1007/s10856-012-4586-6

Cited by 36 publications

(28 citation statements)

References 34 publications

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“…The result from this study is consistent with many previous studies [26,29,52], which have demonstrated that there was a difference in FITR profile between chitosan and CLCG. The peaks of chitosan at 1363 cm −1 and 1155 cm −1 disappeared, indicating that the two peaks may be hindered by glutaraldehyde cross-linked structure of chitosan.…”

Section: Resultssupporting

confidence: 93%

“…Interestingly, previous studies have revealed that the biological activities of chitosan and its derivatives could be affected by different environments [14,21] and improved by either combining chitosan with different metal ions [22,23,24,25] or cross-linking chitosan with other organic compounds [26,27,28]. Indeed, chitosan has been cross-linked with glutaraldehyde in several studies [27,29], while these cross-linked complexes have been found to play a key role in the uptake of heavy metals [30,31].…”

Section: Introductionmentioning

confidence: 99%

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Synthesis, Characterization, and Antibacterial Activity of Cross-Linked Chitosan-Glutaraldehyde

et al. 2013

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This present study deals with synthesis, characterization and antibacterial activity of cross-linked chitosan-glutaraldehyde. Results from this study indicated that cross-linked chitosan-glutaraldehyde markedly inhibited the growth of antibiotic-resistant Burkholderia cepacia complex regardless of bacterial species and incubation time while bacterial growth was unaffected by solid chitosan. Furthermore, high temperature treated cross-linked chitosan-glutaraldehyde showed strong antibacterial activity against the selected strain 0901 although the inhibitory effects varied with different temperatures. In addition, physical-chemical and structural characterization revealed that the cross-linking of chitosan with glutaraldehyde resulted in a rougher surface morphology, a characteristic Fourier transform infrared (FTIR) band at 1559 cm−1, a specific X-ray diffraction peak centered at 2θ = 15°, a lower contents of carbon, hydrogen and nitrogen, and a higher stability of glucose units compared to chitosan based on scanning electron microscopic observation, FTIR spectra, X-ray diffraction pattern, as well as elemental and thermo gravimetric analysis. Overall, this study indicated that cross-linked chitosan-glutaraldehyde is promising to be developed as a new antibacterial drug.

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization, and Antibacterial Activity of Cross-Linked Chitosan-Glutaraldehyde

et al. 2013

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“…When comparing the present results with the previous work carried out with malonic acid (three carbons) Mitra et al (2012), no significant differences in mechanical and thermal stability were observed. However, when comparing the pore size (16 to 18 μm) determination from SEM results of malonic acid cross-linked chitosan/collagen, an increased pore size (27 to 30 μm) was observed in SACCH and SACC scaffolds.…”

Section: Resultssupporting

confidence: 71%

“…In our previous study, we detailed the cross-linking chemistry between malonic acid (MA) with chitosan/collagen Mitra et al (2012). Observations with short-chain dicarboxylic acid (MA, three carbons) cross-linked chitosan/collagen scaffold gave impetus to carry out further work with long-chain dicarboxylic acids, namely sebacic acid (SA) (ten carbons).…”

Section: Introductionmentioning

confidence: 99%

Engineering of chitosan and collagen macromolecules using sebacic acid for clinical applications

2013

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Transformation of natural polymers to three-dimensional (3D) scaffolds for biomedical applications faces a number of challenges, viz., solubility, stability (mechanical and thermal), strength, biocompatibility, and biodegradability. Hence, intensive research on suitable agents to provide the requisite properties has been initiated at the global level. In the present study, an attempt was made to engineer chitosan and collagen macromolecules using sebacic acid, and further evaluation of the mechanical stability and biocompatible property of the engineered scaffold material was done. A 3D scaffold material was prepared using chitosan at 1.0% (w/v) and sebacic acid at 0.2% (w/v); similarly, collagen at 0.5% (w/v) and sebacic acid at 0.2% (w/v) were prepared individually by freeze-drying technique. Analysis revealed that the engineered scaffolds displayed an appreciable mechanical strength and, in addition, were found to be biocompatible to NIH 3T3 fibroblast cells. Studies on the chemistry behind the interaction and the characteristics of the cross-linked scaffold materials suggested that non-covalent interactions play a major role in deciding the property of the said polymer materials. The prepared scaffold was suitable for tissue engineering application as a wound dressing material.Electronic supplementary materialThe online version of this article (doi:10.1186/2194-0517-2-11) contains supplementary material, which is available to authorized users.

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“…Moreover, malonic acid binds between chitosan chains as can be seen in Scheme (2) to allow the attack of E.coli to be by two NH 3 + groups in one time (45) .…”

Section: Scheme 1 Chitosan In N-(carboxy Methyl)-nn-diethyl Benzen mentioning

confidence: 99%

A Study on The Effect of Type of Solvent on Chitosan Efficiency for Treatment of Drinking Water Contaminations

2014

Egypt. J. Chem.

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N this research, chitosan (Ch) a natural polymer, was employed in drinking raw water treatment of Nile River against Escherichia coli via simple method, it is dissolution of chitosan in three different solvents;acetic acid (AC),malonic acid (MA) and N carboxymethyl N,N diethylbenzene ammonium chloride (CMEBAC) to produce three different solutions; Ch-AC, Ch-MA and Ch-CMEBAC, respectively. These solutions were characterized by FTIR and elemental analysis. The investigations of their effect against total coliform bacteria of raw water were carried out according to method of Most Probable Number (MPN) at different contact times (30, 60 and 120 min). The results showed that the full inhibition of total coliform were achieved after 30 min as contact time by 7 gm L -1 ml of Ch-Ac , 5 gm L -1 of Ch-Ma and 0.75 gm L -1 ml of Ch-CMEBAC. Otherwise, by increasing of contact time, the volumes of inhibitors (chitosan solutions) decrease.The minimum inhibitory concentration (MIC) values of those solutions were detected as 2.75, 2 and 0.2 µ/ml , respectively.

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Preparation and characterization of malonic acid cross-linked chitosan and collagen 3D scaffolds: an approach on non-covalent interactions

Cited by 36 publications

References 34 publications

Synthesis, Characterization, and Antibacterial Activity of Cross-Linked Chitosan-Glutaraldehyde

Synthesis, Characterization, and Antibacterial Activity of Cross-Linked Chitosan-Glutaraldehyde

Engineering of chitosan and collagen macromolecules using sebacic acid for clinical applications

A Study on The Effect of Type of Solvent on Chitosan Efficiency for Treatment of Drinking Water Contaminations

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