Preparation and characterization of mesoporous bioactive glass/polycaprolactone nanofibrous matrix for bone tissues engineering

Abstract

A polycaprolactone (PCL) nanofibrous composite matrix having mesoporous bioactive glass nanoparticles (MBG) was fabricated using the electrospinning method, and the microstructural, physical and biological properties of the composite matrix were characterized. The fiber diameters of PCL, 5 % MBG/PCL (5 M-PCL) and 10 % MBG/PCL (10 M-PCL) were 575 ± 162 nm, 312 ± 134 nm and 321 ± 144 nm, respectively. The bioactivity of the composite matrix was evaluated by soaking the matrix in 1.5× simulated body fluid; the MBG/PCL matrix showed a better biomineralization capability than did the PCL matrix. The biological performance of the PCL and the MBG/PCL were evaluated using an in vitro culture of MG63 osteoblast-like cells. We found that the cell attachment and proliferation rates were significantly higher on the 10 M-PCL than on the PCL. Moreover, the expression of several genes, including ANX-V, type I collagen and OCN, ALP activity, the deposition of calcium, and the BSP protein, were also significantly higher on 10 M-PCL than PCL. These results indicated that MBG/PCL has the ability to support cell attachment, growth, and differentiation and can also yield high bioactivity. Therefore, MBG/PCL could be potentially applied in bone implants.

Siliceous mesostructured cellular foams/ poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) composite biomaterials for bone regeneration

“…Enhanced attachment, proliferation, and early differentiation of hMSCs on the SMC/ PHBHHx may result from the direct contact of seeded cells with the SMC particles exposed on the scaffold surface, which might provide cell adhesion sites with better cellular adherence and spreading to stimulate cell proliferation and osteogenic differentiation. 31,32 In addition, SMC/PHBHHx has a higher surface area than pure PHBHHx matrix; most researchers believe that this may promote cell growth more efficiently than a bulk substrate. We also determined that the percentage of hMSCs attached on the SMC/PHBHHx composite with 20 wt% SMC was higher than on pure PHBHHx.…”

Bioactive Glass-Biopolymer Composites

“…the polymer and inorganic particles, the BG loading up to 15 wt% led to the improvement of the tensile strength however simultaneously to a decrease in strain [71].…”

Bioactive Glass-Biopolymer Composites for Applications in Tissue Engineering

“…Kouhi et al [70] and Lin et al [71] both fabricated sol-gel-derived bioactive glass/PCL fibrous scaffolds through electrospinning from a solution of PCL and glass nanoparticles. The study of Kouhi et al showed that bioactivity and cell differentiation activity were significantly improved due to the nanosized bioactive glass addition.…”

“…7) with controlled simvastatin-releasing property. Due to the suitable physical interactions between the polymer and inorganic particles, the BG loading up to 15 wt% led to the improvement of the tensile strength however simultaneously to a decrease in strain [71].…”

“…Unlike the aforementioned strategies, in their study, the 58SiO 2 -38CaO-4P 2 O 5 bioactive glass nanosized fiber mats were first fabricated through the sol-gel Fig. 7 (a) SEM of electrospun PCL fiber mats with 10 wt% mesoporous BG powder fabricated by the direct mixing method, scale of 30 μm, and (b) TEM image of a single fiber which shows that the mesoporous glass nanoparticles were attached to the fiber surfaces [71] (Reproduced with permission from Springer) electrospinning method followed by sintering at 700 C. Subsequently, different fractions of fiber mats were fully soaked and infiltrated with PLA/THF solutions and then dried. These procedures were followed by a thermal-compression process at 130 C to obtain a homogeneous dense plate material.…”