Preparation and Characterization of Microwave‐treated Carboxymethyl Chitin and Carboxymethyl Chitosan Films for Potential Use in Wound Care Application

Abstract

CM-chitin and CM-chitosan films were successfully crosslinked by microwave treatment. Crosslinking of the microwave-treated CM-chitin films involved mainly the carboxylate and the secondary alcohol groups, while crosslinking of microwave-treated CM-chitosan films involved the carboxylate and the amino groups. In addition, the crystallinity of CM-chitin increased with increasing microwave treatment time, whereas an increase in the crystallinity of the microwave-treated CM-chitosan films was not observed. At a similar percentage of weight loss, the crosslinking of either CM-chitin or CM-chitosan films by microwave treatment required much less stringent condition when compared with the crosslinking by autoclave treatment. Based on both direct and indirect cytotoxicity assays, the cytotoxicity of the microwave-treated CM-chitin films was negative, while that of the microwave-treated CM-chitosan films was positive. Human fibroblasts adhered on the surface of microwave-treated CM-chitosan films much better than on the surface of microwave-treated CM-chitin films.

Membrane thickness is an important variable in membrane scaffolds: Influence of chitosan membrane structure on the behavior of cells

“…For example, it has been shown for osteoblast precursor cells that amorphous hydroxyapatite substrates enhanced initial cell attachment compared to highly crystalline hydroxyapatite [5, 6]. In addition, it was reported that fibroblast attachment decreased when a more crystalline carboxymethyl-chitin membrane was used instead of a less crystalline carboxymethyl-chitosan film as the culture substrate [7]. Polymer surface crystallinity was also found to affect the endochondral ossification of rabbit articular cartilage chondrocytes [8].…”

Biomedical applications of carboxymethyl chitosans

“…Also optimum conditions for the production of CM-chitin (with MW = 4.11 × 10 6 Da, viscosity = 1926 cP and DA = 45%) were found to be 60% NaOH concentration at 35-40 • C reaction temperature. The effect of DS of insoluble CM-chitin on the calcium uptake has been investigated and it was concluded that CM-chitin may be useful as a platform for in vivo calcification (Wan, Khor, Wong, & Hastings, 1996) .The suitability of CM-chitin as material for biomedicine particularly in wound healing applications (Wongpanit et al, 2005) and as bioactive bone repairing material has also been shown (Kokubo et al, 2004).…”

“…The potential application of microwave treated CM-chitin and CM-chitosan based films for wound care have been tested (Wongpanit et al, 2005). The in vitro study of 1-ethyl-3(3-dimethylaminopropyl) carbodiimide hydrochloride cross linked (2007) OCM-chitosan Cholesterol The size of novel self aggregated cholesterol succinyl-O,CM-chitosan could be controlled by DS of cholesterol moiety.…”

Synthesis and properties of a water soluble graft (chitosan-g-2-acrylamidoglycolic acid) copolymer

“…It is unique basic polysaccharide in nature because of presence of amino group NH2 in its backbone. It is apolymer of ␤-(1, 4) linked-2-amino-2-deoxyd-glucopyranose derived by N-deacetylation of chitin, abundant naturally occurring polysaccharide with annual production very near the levels of cellulose which consists of mainly ␤-(1-4)-2-deoxy-d-glucose units in aqueous alkaline medium [19][20][21][22]. It occurs in marine invertebrates and shells of marine crustaceans i.e.…”