Preparation and characterization of monoclonal antibody specific for copper–chelate complex

Detection of Cystatin C biomarker for clinical measurement of renal disease by developed ELISA diagnostic kits

“…The first doses contained complete Freund's adjuvant, and subsequent doses were given a 3-weeks interval using incomplete Freund's adjuvant. Blood samples were collected one week after the fourth injection, and the titer and specificity of antibody response were determined with indirect ELISA [ 34 , 35 ].…”

“…After 14 days, ascites fluid was collected 14 days after the injection with cells and centrifugated at 4000 r/min for 15 min. McAbs were purified from mouse ascites by ammonium sulfate precipitation followed by affinity chromatography on a protein G column [ 30 , 34 – 37 ]. Classes and subclasses of McAbs were identified by mouse monoclonal antibody isotyping reagents (Sigma-Aldrich) following the manufacturer’s instructions [ 33 ].…”

“…After then, 2 × 10 12 KM13 helper phages were added, mixed, and incubated for 1 h. Discard supernatant and resuspend pellets in 5 ml of 2 × TY medium, incubated for about 18 h, and then separated and transferred. The phage clones were tested as described previously [ 34 , 39 ]. Maxisorp 96-well plates (Nunc) were coated with HCC and added the antigen at 1 μg/ml.…”

“…The affinity of VHHs was determined as described previously [ 34 , 39 ]. Plates were coated with N-HCC after blocking and washing.…”

A rapid, eco-friendly, and reliable microplate method for determination of Cr(VI)

“…In addition, the use of environmentally friendly procedures is required for International Organization for Standardization (ISO) 14000 certification. 17 Thus, the study of analytes of environmental importance, such as herbicides, 18 cadmium, 19,20 mercury, 21,22 chromium, 5 and copper, 23 using techniques such as the enzymelinked immunosorbent assay (ELISA), is becoming increasingly important due to the advantages that they present, such as speed, sensitivity, specificity, the small volumes of sample required, simultaneous analysis of multiple samples, and the use of low-cost equipment for implementation. 24,25 This work presents the results of applying analytical methodology for the determination of Cr(VI) based on the reaction with DPC in microplates (ELISA microtiter plate) followed by quantification in a reader plate device at 540 nm.…”