Preparation and characterization of novel macroporous cellulose beads regenerated from ionic liquid for fast chromatography

Du, Kaifeng; Yan, Min; Wang, Quanyi; Song, Hang

doi:10.1016/j.chroma.2009.12.037

Cited by 79 publications

(52 citation statements)

References 25 publications

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“…The slope of the curves is the permeability coefficient, which is equal to 0.95 × 10 −7 cm 2 and 1.51 × 10 −7 cm 2 for the 5-mm and 50-mm diameter columns, respectively. These obtained values are between one-and three-times greater than the literature values of membranes developed for similar purposes [35,61] and between one-and two-times greater than the values of columns packed with ion exchange beads [62,63]. The pressure flow curves can also be accounted for until the critical flow velocity is reached, when the pressure drop rises to an extreme value; however, this needs further investigation due to the limitation of system compatibility.…”

Section: Pressure Drop Measurementsmentioning

confidence: 79%

“…In another experiment, Q fibrous adsorbents showed nearly identical capacity at a slow flow rate of 75 cm/h and relatively higher capacity compared to Q Sepharose FF with increasing flow rates, even up to 600 cm/h. The resultant values from Table 1 demonstrate that the fibrous adsorbent capacity is maintained and nearly independent of higher flow rates when compared to packed-bed beads where the capacity is reduced by half with an increase in flow rates [63]. The values reported are also higher compared to strong anion exchange monolithic adsorbents (30 mg/mL) of CIM-QA (BIA Separations, Villach, Austria) and 40 mg/mL for UNO-Q monoliths (Bio-Rad, Hercules, CA, USA) [66].…”

Section: Chromatography Performancementioning

confidence: 96%

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Chromatographic Characterization and Process Performance of Column-Packed Anion Exchange Fibrous Adsorbents for High Throughput and High Capacity Bioseparations

et al. 2015

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Fibrous materials are prominent among novel chromatographic supports for the separation and purification of biomolecules. In this work, strong anion exchange, quaternary ammonium (Q) functional fibrous adsorbents were evaluated with regards to their physical and functional characteristics. A column packed with Q fibrous adsorbent illustrated the good column packing efficiency of theoretical plate height (H) values and higher permeability coefficients (>0.9 × 10 −7 cm 2 ) than commercial adsorbents. For pulse experiments with acetone and lactoferrin as tracers under nonbinding conditions, the total porosity (for acetone) and the interstitial porosity (for lactoferrin) measured 0.97 and 0.47, respectively. The total ionic capacity of the chemically-functionalized Q fiber was 0.51 mmol/mL. The results indicated that the Q fiber had a static binding capacity of 140 mg/mL and a dynamic binding capacity (DBC) of 76 mg/mL for bovine serum albumin (BSA) and showed a linearly-scalable factor (~110 mL) for a column volume with high capacity and high throughput. Furthermore, this adsorptive material had the ability to bind OPEN ACCESSProcesses 2015, 3 205 the high molecular weight protein, thyroglobulin, with a capacity of 6 mg/mL. This work demonstrated the column-packed Q fibrous adsorption system as a potential chromatography support that exhibits high capacity at higher flow rates.

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Section: Pressure Drop Measurementsmentioning

confidence: 79%

Section: Chromatography Performancementioning

confidence: 96%

Chromatographic Characterization and Process Performance of Column-Packed Anion Exchange Fibrous Adsorbents for High Throughput and High Capacity Bioseparations

et al. 2015

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“…After washing with dilute HCl to remove the poreforming agent, various kinds of porous adsorbents could be prepared. Alternatively, macroporous cellulose beads could also be prepared from cellulose solution in ionic liquid by double emulsification [18,19].…”

Section: Preparation Of Cellulosic Beads [10 11]mentioning

confidence: 99%

Bioactive Bead Type Cellulosic Adsorbent for Blood Purification

Wang¹,

Yu²

2013

Cellulose - Medical, Pharmaceutical and Electronic Applications

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“…Among all of these techniques, liquid chromatography (LC) packed with chiral stationary phase (CSPs) is very attractive because it possesses excellent enantioselectivities in a wide range of enantiomers. For this technique, the CSPs are the central unit that relates strongly to the chromatographic performance [7][8][9]. At present, increasing research efforts have been focused on the fabrication of the novel adsorbent possessing of better enantioselectivity, stability and capacity and their potential application in industry.…”

mentioning

confidence: 99%

Fabrication and characterization of novel tentacle-type adsorbent for resolution of chiral drugs

Zhu

Wang

et al. 2013

Chin. Sci. Bull.

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Novel tentacle-type chiral adsorbent based on silica gels modified with β-cyclodextrin (β-CD) and polyvinyl alcohol(PVA) is synthesized by surface grafting technique. The adsorption behavior of chiral pharmaceuticals using the resulting adsorbents is investigated. In the study, FT-IR and thermogravimetric analysis (TGA) are employed to characterize the chemical and physical properties of the adsorbent; mandelic acid (MA) serves as model solute to evaluate the sorption capacity and selectivity of the adsorbent. The experimental results show that the novel tentacle-type adsorbent is possessed of larger equilibrium adsorption capacity and better selectivity over the conventional one and could be as potential chiral stationary phase (CSPs).tentacle-type adsorbent, adsorptive selectivity, chiral resolution, MA Citation:Zhu W X, Wang Q Y, Du K F, et al. Fabrication and characterization of novel tentacle-type adsorbent for resolution of chiral drugs.

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Preparation and characterization of novel macroporous cellulose beads regenerated from ionic liquid for fast chromatography

Cited by 79 publications

References 25 publications

Chromatographic Characterization and Process Performance of Column-Packed Anion Exchange Fibrous Adsorbents for High Throughput and High Capacity Bioseparations

Chromatographic Characterization and Process Performance of Column-Packed Anion Exchange Fibrous Adsorbents for High Throughput and High Capacity Bioseparations

Bioactive Bead Type Cellulosic Adsorbent for Blood Purification

Fabrication and characterization of novel tentacle-type adsorbent for resolution of chiral drugs

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