Preparation and characterization of organosilicon thin films for selective adhesion of Yarrowia lipolytica yeast cells

Abstract

The adhesion of Yarrowia lipolytica and Saccharomyces cerevisiae on organosilicon thin films deposited on polycarbonate substrates was investigated through a series of adhesion tests in order to obtain a selective substrate for Y. lipolytica cell adhesion. Organosilicon thin films were prepared using atmospheric pressure surface barrier discharge. The surface was characterized by its total surface energy with its components calculated using the acid-base theory. Assessment of adhesion via cell surface coverage was obtained by standard tests and also through image analysis of yeast cells on the organosilicon layers. The results show that it is possible to create organosilicon thin films for a selective adhesion of Y. lipolytica face to S. cerevisiae behaviour, for potential use in fixed bed biofilm reactors for industrial purposes involving enzyme-based biotransformations.

Deposition of Yarrowia lipolytica on plasma prepared teflonlike thin films

“…Adhesion efficiency increases up to pH 7 upon which the coverage does not seem to change further as reported in previous studies. 3 This result is in full agreement with the zeta potential measurements previously discussed and shown in Fig. 3.…”

“…3 The films were deposited from mixtures of C 4 F 8 with nitrogen. In the present case the surface power density was kept at 1?5 W cm 22 in all cases.…”

Biocidal action of ozone-treated polystyrene surfaces on vegetative and sporulated bacteria

“…For example, surface modifications with UV/O 3 [1] or with plasmas such as corona discharges [2] can increase proliferation and protein expression of cells or enhance their culture process. Similar action can be obtained with coatings with well-defined surface chemistries as they can also amplify, or prevent, bioadhesion of molecules, cells and, in some cases, bacteria [7][8][9][10].…”

Yarrowia lipolytica as an Oleaginous Cell Factory Platform for Production of Fatty Acid-Based Biofuel and Bioproducts

“…Although this yeast shows promise for biofilm generation, this approach may be inefficient for lipid production under nutrient starvation. However, the Y. lipolytica biofilm on the selective substrate can be more efficiently used in a fixed bed biofilm reactor (Lehocký, 2007). The immobilization technique may also prevent reconsumption of secreted bioproducts.…”