Preparation and characterization of paclitaxel palmitate albumin nanoparticles with high loading efficacy: an in vitro and in vivo anti-tumor study in mouse models

Abstract

Background: Combination of the prodrug technique with an albumin nano drug-loaded system is a novel promising approach for cancer treatment. However, the long-lasting and far-reaching challenge for the treatment of cancers lies in how to construct the albumin nanometer drug delivery system with lead compounds and their derivatives. Methods: In this study, we reported the preparation of injectable albumin nanoparticles (NPs) with a high and quantitative drug loading system based on the Nab TM technology of paclitaxel palmitate (PTX-PA).Results: Our experimental study on drug tissue distribution in vivo demonstrated that the paclitaxel palmitate albumin nanoparticles (Nab-PTX-PA) remained in the tumor for a longer time post-injection. Compared with saline and paclitaxel albumin nanoparticles (Abraxane V R ), intravenous injection of Nab-PTX-PA not only reduced the toxicity of the drug in normal organs, and increased the body weight of the animals but maintained sustained release of paclitaxel (PTX) in the tumor, thereby displaying an excellent antitumor activity. Blood routine analysis showed that Nab-PTX-PA had fewer adverse effects or less toxicity to the normal organs, and it inhibited tumor cell proliferation more effectively as compared with commercial paclitaxel albumin nanoparticles. Conclusions: This carrier strategy for small molecule drugs is based on naturally evolved interactions between long-chain fatty acids (LCFAs) and Human Serum Albumin (HSA), demonstrated here for PTX. Nab-PTX-PA shows higher antitumor efficacy in vivo in breast cancer models. On the whole, this novel injectable Nab-PTX-PA has great potential as an effective drug delivery system in the treatment of breast cancer.

An oncolytic adenovirus coding for a variant interleukin 2 cytokine improves response to chemotherapy through enhancement of effector lymphocyte cytotoxicity, fibroblast compartment modulation and mitotic slippage

“…No signs of toxicity associated with the therapies was observed at histopathological analysis of collected internal organs ( Supplementary Table 2 ), and no toxicity visible by animal external features or behavior were observed, in line with previously published results ( 19 , 20 ).…”

Glycaemia dynamics concepts before and after insulin

“…Albumin is a small (66.7 kDa), negatively charged globular protein released by the liver into the blood (~12.5 mg per day), where it reaches a concentration of ~40 g/L in the serum of healthy adults [35]. Albumin in the circulation acts as a carrier for other endogenous (v.g.…”

The application of nanomedicine in clinical settings

“…For instance, nanomedicines have been widely applied. Since the first nanomedicine doxorubicin (DOX) was introduced in 1995 ( Barenholz, 2012 ), researchers have developed a wide range of nanomedicines to date, including paclitaxel albumin nanoparticles ( Chen et al, 2021 ) and elitecan liposomes ( Wang T. et al, 2021 ), which are used to treat a variety of serious diseases. The use of nanomedicine for disease monitoring and minimally invasive surgery is among the many clinical applications of nanomedicine.…”