Preparation and characterization of phosphatidylcholine‐coated zirconia–magnesia stationary phase for artificial membrane chromatography

Abstract

Immobilized artificial membrane chromatography stationary phase was prepared by coating soybean phosphatidylcholine (PC) on zirconia-magnesia micro-particles. The stability and chromatographic properties were investigated and compared with the PC-coated silica chromatography stationary phase prepared by the same method. PC-coated zirconia-magnesia chromatography stationary phase was more stable than the silica especially on resisting organic solvents. Hydrophobic action was the main factor for the retention of analyte on the new artificial membrane chromatography stationary phase, and electrostatic interaction had some contribution to retention. In addition, the special interaction between analyte and matrix affected retention greatly. Basic solutes were appropriate to be analyzed on PC-coated zirconia-magnesia stationary phase and acidic solutes were appropriate to be done on the silica one. Hence, the two different matrices artificial membrane stationary phases were perfectly complementary.

A novel polychloromethylstyrene coated superparamagnetic surface molecularly imprinted core–shell nanoparticle for bisphenol A

“…However, silica is only stable at pH values from 2 to 8. 40 If the magnetic MIP nanoparticles were treated in extreme pH conditions, the Fe 3 O 4 would be dissolved in acidic conditions, damaging the magnetic response, and the silica shell would be dissolved in basic conditions. Concerning this point, compact encapsulation of Fe 3 O 4 nanoparticles with good chemical stability was essential for the wide use of Fe 3 O 4 @MIP.…”

Thermodynamic study of new antiepileptic compounds by combining chromatography on the phosphatidylcholine biomimetic stationary phase and differential scanning calorimetry

“…IAMs, as new sorbents for HPLC, have been discovered by Pidgeon and Venkataram at the end of the 20th century. IAM stationary phases can be prepared from phospholipids covalently bonded to a propylaminosilica support material, α1‐acid glycoprotein or albumin derivatized surfaces, but some works describe hand‐made preparation of IAM sorbent by for instance coating soybean phosphatidylcholine (PC) on zirconia–magnesia micro‐particles . Several types of IAM column containing PC are now commercially available such as IAMPC‐DD, IAMPC‐MG, and IAMPC‐DD2.…”

Preparation, chromatographic evaluation and application of adenosine 5′-monophosphate modified ZrO2/SiO2 stationary phase in hydrophilic interaction chromatography

“…Taking advantage of this property, various phosphate-containing ZrO 2 -based SPs were prepared using alkyl phosphates [6,15,16], phosphatidylcholine [17], phosphonate-containing BSA [18], ethylenediamine-N,Ntetramethylphosphonic acid [1] and other organic phosphates [19,20] as modifiers. However, all these SPs were hydrophobic and applied in reversed-phase high performance liquid chromatography (RP-HPLC) [16,17], membrane chromatography [17] and capillary electrochromatography [15]. However, less attention was paid to the application of bare ZrO 2 or its modified forms for hydrophilic interaction chromatography (HILIC).…”