1986
DOI: 10.1111/j.1432-1033.1986.tb10125.x
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Preparation and characterization of pig plasma and platelet gelsolins

Abstract: Pig plasma gelsolin has been prepared by a revised method involving poly(ethy1ene glycol) precipitation, chromatography on CM-cellulose and affinity chromatography on actin-Sepharose. Pig platelet gelsolin has been prepared by chromatography on DEAE-cellulose and actin-Sepharose. Partial chemical and proteolytic cleavage shows that the two proteins are closely related in their fragmentation patterns. The amino acid sequences are identical at the N-terminus of the platelet protein, but the plasma protein has an… Show more

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Cited by 49 publications
(39 citation statements)
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“…The determined affinities range from micromolar to millimolar [9,[13][14][15][16]. Values similar to our results were measured by Kilhoffer and GCrard [15] and by Doi et al [16].…”
Section: Discussionsupporting
confidence: 80%
“…The determined affinities range from micromolar to millimolar [9,[13][14][15][16]. Values similar to our results were measured by Kilhoffer and GCrard [15] and by Doi et al [16].…”
Section: Discussionsupporting
confidence: 80%
“…1A), cdc2 kinase is responsible for this gelsolin phosphorylation (Yamakita et al, 1994;Yamashiro et al, 1990Yamashiro et al, , 1991. The consensus phosphorylation sequence of this kinase can be found in gelsolin at several sites (Way and Weeds, 1988;Weeds et al, 1986). Our results, however, show that the phosphorylation site is located near the NH2-terminus of plasma gelsolin, because the CT17N fragment was produced from the NH 2 -terminal region (Fig.…”
Section: Phosphorylation Of Plasma Gelsolincontrasting
confidence: 53%
“…This additional NH 2 -terminus region consists of at least 9 additional amino acid residues and occasionally contains signal and propeptide sequences. The consensus phosphorylation sequence of cdc2 kinase (Ser/Thr-Pro) is found in this region of porcine plasma gelsolin (Way and Weeds, 1988;Weeds et al, 1986;Yamakita et al, 1994;Yamashiro et al, 1990). This leader sequence region of plasma gelsolin is much less conserved and the amino acid sequence of calf serum gelsolin has not yet been solved.…”
Section: Discussionmentioning
confidence: 99%
“…In the results described hitherto the free Ca2+ concentration was about 0.1 mM according to calculations [39]. As the equilibrium constant for dissociation of calcium from gelsolin is about 1 pM [16,24,40,411, almost all gelsolin molecules bound Ca". In Fig.…”
Section: Quantitative Evaluation Of the Time Course Of Formation And mentioning
confidence: 92%