Preparation and characterization of PLA-PEG/Chitosan-FA/DNA for gene transfer to MCF-7 cells

Afrouz, Mehdi; Ahmadi-Nouraldinvand, Farnaz; Ajirlu, Yahia Yaghouti; Arabnejad, Fatemeh; Eskanlou, Hadi; Yaghoubi, Hashem

doi:10.1016/j.medidd.2022.100138

Cited by 3 publications

(1 citation statement)

References 30 publications

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“…However, one major drawback is the low efficacy they demonstrate when used for such purposes. To overcome this issue, Afrouz et al [ 55 ] introduced chitosan–folic acid (CS-FA) to PLA-PEG nanoparticles. The aim of this research was to increase the encapsulation efficiency of DNA within the nanoparticles and increase the protection of DNA from enzyme digestion damage.…”

Section: Chitosan Complexes For Nucleic Acid Deliverymentioning

confidence: 99%

Chitosan-Based Nanoparticles for Nucleic Acid Delivery: Technological Aspects, Applications, and Future Perspectives

et al. 2023

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Chitosan is a naturally occurring polymer derived from the deacetylation of chitin, which is an abundant carbohydrate found mainly in the shells of various marine and terrestrial (micro)organisms. Chitosan has been extensively used to construct nanoparticles (NPs), which are biocompatible, biodegradable, non-toxic, easy to prepare, and can function as effective drug delivery systems. Moreover, chitosan NPs have been employed in gene and vaccine delivery, as well as advanced cancer therapy, and they can also serve as new therapeutic tools against viral infections. In this review, we summarize the most recent developments in the field of chitosan-based NPs intended as nucleic acid delivery vehicles and gene therapy vectors. Special attention is given to the technological aspects of chitosan complexes for nucleic acid delivery.

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Section: Chitosan Complexes For Nucleic Acid Deliverymentioning

confidence: 99%

Chitosan-Based Nanoparticles for Nucleic Acid Delivery: Technological Aspects, Applications, and Future Perspectives

et al. 2023

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Exploring modified chitosan-based gene delivery technologies for therapeutic advancements

Gholap,

Kapare,

Pagar

et al. 2024

International Journal of Biological Macromolecules

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Gene Therapy with Chitosan Nanoparticles: Modern Formulation Strategies for Enhancing Cancer Cell Transfection

Antoniou,

Mourelatou,

Galatou

et al. 2024

Pharmaceutics

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Gene therapy involves the introduction of exogenous genetic material into host tissues to modify gene expression or cellular properties for therapeutic purposes. Initially developed to address genetic disorders, gene therapy has expanded to encompass a wide range of conditions, notably cancer. Effective delivery of nucleic acids into target cells relies on carriers, with non-viral systems gaining prominence due to their enhanced safety profile compared to viral vectors. Chitosan, a biopolymer, is frequently utilized to fabricate nanoparticles for various biomedical applications, particularly nucleic acid delivery, with recent emphasis on targeting cancer cells. Chitosan’s positively charged amino groups enable the formation of stable nanocomplexes with nucleic acids and facilitate interaction with cell membranes, thereby promoting cellular uptake. Despite these advantages, chitosan-based nanoparticles face challenges such as poor solubility at physiological pH, non-specificity for cancer cells, and inefficient endosomal escape, limiting their transfection efficiency. To address these limitations, researchers have focused on enhancing the functionality of chitosan nanoparticles. Strategies include improving stability, enhancing targeting specificity, increasing cellular uptake efficiency, and promoting endosomal escape. This review critically evaluates recent formulation approaches within these categories, aiming to provide insights into advancing chitosan-based gene delivery systems for improved efficacy, particularly in cancer therapy.

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Preparation and characterization of PLA-PEG/Chitosan-FA/DNA for gene transfer to MCF-7 cells

Cited by 3 publications

References 30 publications

Chitosan-Based Nanoparticles for Nucleic Acid Delivery: Technological Aspects, Applications, and Future Perspectives

Chitosan-Based Nanoparticles for Nucleic Acid Delivery: Technological Aspects, Applications, and Future Perspectives

Exploring modified chitosan-based gene delivery technologies for therapeutic advancements

Gene Therapy with Chitosan Nanoparticles: Modern Formulation Strategies for Enhancing Cancer Cell Transfection

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