Preparation and characterization of poly(<scp>L</scp>‐lactide)/ poly(ϵ‐caprolactone) fibrous scaffolds for cartilage tissue engineering

Zhao, Jin; Yuan, Xiaoyan; Cui, Yuan‐Lu; Ge, Quanbo; Yao, Kangde

doi:10.1002/app.13323

Cited by 44 publications

(44 citation statements)

References 26 publications

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“…When T gel ≥4ºC, no gelation phenomena were observed in the PCL (M n : 80k)/THF system (5%, w/v). Zhao et al (Zhao et al, 2004) also documented that the gelation time of the PCL/THF system was 60min (T gel : -18ºC), longer than the gelation time of the PLLA/THF system at the same T gel , which was 22min. In this study, we found that increasing T gel would accelerate molecular movement which would not favor the microphase separation of the PCL domain from the PLLA domain in the PCL-b-PLLA/THF system.…”

Section: He Et Almentioning

confidence: 84%

“…Due to the mechanism of nanofiber fabrication by TIPS, semicrystalline homopolymers or copolymers which would crystallize and form microcrystalline domains during the TIPS process, were indispensable. PLLA/PCL blends were also utilized to prepare 3D nano-fibrous scaffolds by L-L TIPS from a THF solution (Zhao et al, 2004). However, PLLA with a high molecular weight could usually not be mixed with PCL, and the adhesion between PCL and PLLA became weak, resulting in poor mechanical properties (Kim et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Microstructure and properties of nano-fibrous PCL-b-PLLA scaffolds for cartilage tissue engineering

Liu²,

Guan³

et al. 2009

eCM

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Nano-fibrous scaffolds which could potentially mimic the architecture of extracellular matrix (ECM) have been considered a good candidate matrix for cell delivery in tissue engineering applications. In the present study, a semicrystalline diblock copolymer, poly(ε-caprolactone)-block-poly(L-lactide) (PCL-b-PLLA), was synthesized and utilized to fabricate nano-fibrous scaffolds via a thermally induced phase separation process. Uniform nano-fibrous networks were created by quenching a PCL-b-PLLA/THF homogenous solution to -20ºC or below, followed by further gelation for 2 hours due to the presence of PLLA and PCL microcrystals. However, knot-like structures as well as continuously smooth pellicles appeared among the nanofibrous network with increasing gelation temperature. DSC analysis indicated that the crystallization of PCL segments was interrupted by rigid PLLA segments, resulting in an amorphous phase at high gelation temperatures. Combining TIPS (thermally induced phase separation) with saltleaching methods, nano-fibrous architecture and interconnected pore structures (144±36 μm in diameter) with a high porosity were created for in vitro culture of chondrocytes. Specific surface area and protein adsorption on the surface of the nano-fibrous scaffold were three times higher than on the surface of the solid-walled scaffold. Chondrocytes cultured on the nano-fibrous scaffold exhibited a spherical condrocyte-like phenotype and secreted more cartilage-like extracellular matrix (ECM) than those cultured on the solid-walled scaffold. Moreover, the protein and DNA contents of cells cultured on the nanofibrous scaffold were 1.2-1.4 times higher than those on the solid-walled scaffold. Higher expression levels of collagen II and aggrecan mRNA were induced on the nanofibrous scaffold compared to on the solid-walled scaffold. These findings demonstrated that scaffolds with a nanofibrous architecture could serve as superior scaffolds for cartilage tissue engineering.

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Section: He Et Almentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Microstructure and properties of nano-fibrous PCL-b-PLLA scaffolds for cartilage tissue engineering

Liu²,

Guan³

et al. 2009

eCM

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“…These constitute obvious interesting questions in tissue engineering. Although "wet-state" mechanical properties of crystalline polyesters such as poly(llactide) (PLLA) under aqueous environments have been reported, 32,33 publication specifically for a thorough investigation of "wet-state" mechanical properties of tissue engineering scaffolds is still quite limited.…”

Section: Introductionmentioning

confidence: 99%

“Wet‐state” mechanical properties of three‐dimensional polyester porous scaffolds

Zhang

Jing

et al. 2005

J Biomedical Materials Res

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Porous poly(d,l-lactic-co-glycolic acid) (PLGA) scaffolds under a simulated physiological environment were investigated to estimate their "true" mechanical properties, with emphasis on the effect of "wet-state" on the compressive behaviors. The effect of the history of ethanol sterilization was also investigated. The studies were focused upon the "wet-state" mechanical properties of polyester porous scaffolds, because the potential implants must be used under a wet environment. The measurements of three-dimensional porous scaffolds composed of amorphous PLGA with five polymer formulations including poly(d,l-lactic acid) (PDLLA) demonstrated that the mechanical properties of PLGA scaffolds significantly decreased in phosphate buffer saline solution (PBS) at 37°C and/or with an ethanol sterilization history, even though PLGA is a hydrophobic material. The decrease extent depends on the copolymer composition: when the porosity is about 90%, a PDLLA scaffold remained about 75-80% of initial mechanical properties in the dry state at 25°C, whereas PLGA 85:15, 75:25, and 65:35 scaffolds remained only about 10% or less, and the PLGA 50:50 scaffolds examined were not sufficiently strong for mechanical tests. If scaffolds were prewetted with ethanol ahead of prewetting with PBS, the mechanical properties further decreased compared with those merely prewetted with PBS. These phenomena were elucidated experimentally from plasticization of PLGA with water or ethanol, and the consequent reduction of glass transition temperature. The results might be helpful for designing polyester porous scaffolds for tissue engineering or in situ tissue induction applications.

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“…Many groups have reported the mechanical properties of porous scaffolds of different biomaterials fabricated by different approaches [9,15,17,18,[31][32][33][34][35], including our group [20 -22,26,27,36,37]. The mechanical properties of the porous scaffolds could be affected by several factors, and some factors were not well recognized or understood.…”

Section: Mechanical Properties Of Porous Scaffoldsmentioning

confidence: 99%

Poly(lactide- co -glycolide) porous scaffolds for tissue engineering and regenerative medicine

2012

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Porous scaffolds fabricated from biocompatible and biodegradable polymers play vital roles in tissue engineering and regenerative medicine. Among various scaffold matrix materials, poly(lactide-co-glycolide) (PLGA) is a very popular and an important biodegradable polyester owing to its tunable degradation rates, good mechanical properties and processibility, etc. This review highlights the progress on PLGA scaffolds. In the latest decade, some facile fabrication approaches at room temperature were put forward; more appropriate pore structures were designed and achieved; the mechanical properties were investigated both for dry and wet scaffolds; a long time biodegradation of the PLGA scaffold was observed and a three-stage model was established; even the effects of pore size and porosity on in vitro biodegradation were revealed; the PLGA scaffolds have also been implanted into animals, and some tissues have been regenerated in vivo after loading cells including stem cells.

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Preparation and characterization of poly(L‐lactide)/ poly(ϵ‐caprolactone) fibrous scaffolds for cartilage tissue engineering

Cited by 44 publications

References 26 publications

Microstructure and properties of nano-fibrous PCL-b-PLLA scaffolds for cartilage tissue engineering

Microstructure and properties of nano-fibrous PCL-b-PLLA scaffolds for cartilage tissue engineering

“Wet‐state” mechanical properties of three‐dimensional polyester porous scaffolds

Poly(lactide- co -glycolide) porous scaffolds for tissue engineering and regenerative medicine

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