Preparation and characterization of polysaccharidic microbeads by a microfluidic technique: Application to the encapsulation of Sertoli cells

Capretto, Lorenzo; Mazzitelli, Stefania; Luca, Giovanni; Nastruzzi, Claudio

doi:10.1016/j.actbio.2009.08.023

Cited by 38 publications

(23 citation statements)

References 21 publications

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“…Because of the hydrophilic and biocompatible nature of hydrogels, encapsulated cells can maintain high viability and normal functions for extended period of time 28, 153–155 . Their homogenous size and controllable morphology also improve in vivo injectability and facilitate 3D imaging 51, 156 .…”

Section: Hydrogel Microencapsulation For 3d Cell Culturementioning

confidence: 99%

Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture

et al. 2017

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Hydrogel microcapsules provide miniaturized and biocompatible niches for three-dimensional (3D) in vitro cell culture. They can be easily generated by droplet-based microfluidics with tunable size, morphology, and biochemical properties. Therefore, microfluidic generation and manipulation of cell-laden microcapsules can be used for 3D cell culture to mimic the in vivo environment towards applications in tissue engineering and high throughput drug screening. In this review of recent advances mainly since 2010, we will first introduce general characteristics of droplet-based microfluidic devices for cell encapsulation with an emphasis on the fluid dynamics of droplet breakup and internal mixing as they directly influence microcapsule’s size and structure. We will then discuss two on-chip manipulation strategies: sorting and extraction from oil into aqueous phase, which can be integrated into droplet-based microfluidics and significantly improve the qualities of cell-laden hydrogel microcapsules. Finally, we will review various applications of hydrogel microencapsulation for 3D in vitro culture on cell growth and proliferation, stem cell differentiation, tissue development, and co-culture of different types of cells.

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Section: Hydrogel Microencapsulation For 3d Cell Culturementioning

confidence: 99%

Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture

et al. 2017

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“…[20][21][22][23][24][25] For example, many research groups developed microfluidics devices to produce functionalized polymer microspheres 21,26 and inorganic nanoparticles, [27][28][29][30] with precise control over their shapes and sizes. Song et al reviewed various microfluidics synthesis techniques to form metallic and silica nanoparticles and quantum dots.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic one-step fabrication of radiopaque alginate microgels with in situ synthesized barium sulfate nanoparticles

Wang

Zhang

et al. 2012

Lab Chip

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In this work, we report a new strategy to fabricate monodispersed radiopaque alginate (Ba-alginate) microgels by a one-step microfluidic method. Alginate droplets containing sulfate ions are first formed by a flow focusing microfluidic setup. These alginate droplets are subsequently solidified by barium ions in a collection bath. During the solidification process, excessive barium ions in the collection bath also react with sulfate ions in the alginate droplet, resulting in barium sulfate (BaSO(4)) nanoparticles in situ synthesized (acting as radiopaque imaging agents) within the Ba-alginate microgels. Scanning electron microscopy (SEM) combined with energy-dispersive X-ray spectroscopy (EDX) illustrate that 800 nm BaSO(4) nanoparticles are uniformly distributed inside the 30 μm Ba-alginate microgels, with 62 wt% of elemental barium (Ba). In addition, X-ray diffraction (XRD) measurements indicate that the BaSO(4) nanoparticles consist of 10 nm in situ synthesized BaSO(4) crystallites. The alginate microgels act as a soft and porous template to prevent the precipitation and aggregation of BaSO(4) nanoparticles. The Ba-alginate microgels are also visible under X-ray radiation. The facile route to fabricate alginate microgels as radiopaque embolic materials is of particular importance for endovascular embolization and localized diagnostic imaging applications. Similar approaches can also be adopted for synthesizing other inorganic nanoparticles in microgels.

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“…Microfluidic (This manuscript) Microfluidic [15,[45][46][47] Nozzle-based droplet generator [48,49] Microfluidic [41,50] Microfluidic [18,19] Crosslinking method Photo Chemical Chemical Thermal Photo separated into aqueous and organic phase. The organic phase was collected and dried with anhydrous MgSO 4 (Fisher).…”

Section: Production Methods (References)mentioning

confidence: 99%

Rapid Production of Cell‐Laden Microspheres Using a Flexible Microfluidic Encapsulation Platform

Seeto

Tian

Pradhan

et al. 2019

Small

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This study establishes a novel microfluidic platform for rapid encapsulation of cells at high densities in photocrosslinkable microspherical hydrogels including poly(ethylene glycol)‐diacrylate, poly(ethylene glycol)‐fibrinogen, and gelatin methacrylate. Cell‐laden hydrogel microspheres are advantageous for many applications from drug screening to regenerative medicine. Employing microfluidic systems is considered the most efficient method for scale‐up production of uniform microspheres. However, existing platforms have been constrained by traditional microfabrication techniques for device fabrication, restricting microsphere diameter to below 200 µm and making iterative design changes time‐consuming and costly. Using a new molding technique, the microfluidic device employs a modified T‐junction design with readily adjustable channel sizes, enabling production of highly uniform microspheres with cell densities (10–60 million cells mL−1) and a wide range of diameters (300–1100 µm), which are critical for realizing downstream applications, through rapid photocrosslinking (≈1 s per microsphere). Multiple cell types are encapsulated at rates of up to 1 million cells per min, are evenly distributed throughout the microspheres, and maintain high viability and appropriate cellular activities in long‐term culture. This microfluidic encapsulation platform is a valuable and readily adoptable tool for numerous applications, including supporting injectable cell therapy, bioreactor‐based cell expansion and differentiation, and high throughput tissue sphere‐based drug testing assays.

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Preparation and characterization of polysaccharidic microbeads by a microfluidic technique: Application to the encapsulation of Sertoli cells

Cited by 38 publications

References 21 publications

Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture

Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture

Microfluidic one-step fabrication of radiopaque alginate microgels with in situ synthesized barium sulfate nanoparticles

Rapid Production of Cell‐Laden Microspheres Using a Flexible Microfluidic Encapsulation Platform

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