Preparation and Characterization of Protein Molecularly Imprinted Poly (Ionic Liquid)/Calcium Alginate Composite Cryogel Membrane with High Mechanical Strength for the Separation of Bovine Serum Albumin

Fan, Jie; Dong, Wenchen; Zhang, Xuehong; Yu, Jiaxin; Huang, Cong‐Bo; Deng, Lijuan; Chen, Huiping; Peng, Hailong

doi:10.3390/molecules27217304

Cited by 8 publications

(6 citation statements)

References 40 publications

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“…These results indicated that BSA-PC-MIP was robust and had excellent reusability. Very differently, an obvious decrease in adsorption capability was observed for some previously developed protein-imprinted polymers, including BSA MIPs. , …”

Section: Resultsmentioning

confidence: 93%

“…Very differently, an obvious decrease in adsorption capability was observed for some previously developed protein-imprinted polymers, including BSA MIPs. 16,50 BSA Separation from Fetal Bovine Serum. The BSA-PC-MIP was then used to isolate BSA from fetal bovine serum.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

“…One challenge is the difficult removal of the template because of the large size of the protein . The second challenge is the low imprinting efficiency which is attributed to the sparsely cross-linked networks of the polymers, the attenuated interactions between protein template and functional monomer in aqueous solutions, and the pliable structure of protein molecules. ,, To solve these problems, functional monomers such as polymerizable ionic liquid monomers were used to improve imprinting efficiency. − Advanced strategies, including surface imprinting − and epitope imprinting, were also adopted to synthesize BSA MIPs. However, the problems were not fully overcome.…”

Section: Introductionmentioning

confidence: 99%

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Molecularly Imprinted Polymer with High Capacity and Selectivity for Separation of Bovine Serum Albumin

Zhang,

Wang,

et al. 2023

ACS Appl. Polym. Mater.

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A cost-effective but simple method for the separation of bovine serum albumin (BSA) from complex biological fluids is still lacking. Here using N-isopropylacrylamide as the main monomer and dimethylaminoethyl methacrylate, methacrylic acid, and acrylamide as functional monomers, a BSA-imprinted polymer was synthesized. To overcome the two major obstacles of common protein-imprinted polymers, i.e., difficult template removal and low imprinting efficiency, an oligo(glutamic acid)-based peptide cross-linker with the same vinyl group on both ends was designed and used as cross-linker in the synthesis of the polymers. The introduction of the peptide segments, which are capable of undergoing a pH-triggered reversible and precise helix−coil transition, into the polymers makes the imprint cavities shape-memorable. Therefore, complete removal of the template BSA under mild conditions, that is, at physiological pH, ionic strength, and temperature, was achieved. More importantly, an adsorption capacity of 607 mg/g and an imprinting factor of 6.0, both of which are not only higher than the molecularly imprinted polymer (MIP) cross-linked with the commonly used cross-linker N,N′-methylenebis(acrylamide), but also higher than most BSA MIPs reported previously in the literature, were obtained, indicating a high affinity of the MIP to the template BSA. The BSA MIP also showed a high selectivity and could be used repeatedly. Using the BSA MIP as the adsorbent, BSA was extracted from fetal bovine serum selectively and efficiently.

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Section: Resultsmentioning

confidence: 93%

Section: ■ Experimental Sectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecularly Imprinted Polymer with High Capacity and Selectivity for Separation of Bovine Serum Albumin

Zhang,

Wang,

et al. 2023

ACS Appl. Polym. Mater.

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“…Then, the pseudo-first-order and pseudo-second-order kinetic models are applied to investigate the controlling mechanism of the binding process (Table , eqs S3 and S4). , In general, the pseudo-first-order model supposes that the adsorption process is based on physical adsorption, whereas the pseudo-second-order model assumes that the adsorption rate is governed by chemical adsorption . According to the correlation coefficient ( R 2 ) value, the pseudo-second-order kinetic model better fits the binding process of EIMS for Cyt c , demonstrating that Cyt c can complementally match the imprinted sites via multiple interactions to achieve fast, efficient, and accurate adsorption.…”

Section: Resultsmentioning

confidence: 99%

Enhancing Cytochrome C Recognition and Adsorption through Epitope-Imprinted Mesoporous Silica with a Tailored Pore Size

Cheng,

Han,

Zou

et al. 2023

ACS Omega

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We have reported the synthesis of epitope-imprinted mesoporous silica (EIMS) with an average pore size of 6.2 nm, which is similar to the geometrical size of the target protein, cytochrome C (Cyt c, 2.6 × 3.2 × 3.3 nm3), showing great recognition and large-scale adsorption performance. The characteristic fragment of Cyt c was used as a template and docked onto the surface of C16MIMCl micelles via multiple interactions. Nitrogen adsorption–desorption and transmission electron microscopy confirmed the successful preparation of EIMS. Due to the ordered pore structure, larger pore size, and high specific surface area, the prepared EIMS show superior specificity (IF = 3.8), excellent selectivity toward Cyt c, high adsorption capacity (249.6 mg g–1), and fast adsorption equilibrium (10 min). This study demonstrates the potential application of EIMS with a controllable pore size for high-effective and large-scale separation of Cyt c, providing a new approach for effective biomacromolecular recognition.

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“…to form the host-guest complex; addition of a crosslinker and initiation through certain initiation methods (e.g., initiator, thermal initiation, photoinitiation, etc.) to produce a highly cross-linked polymer; the template molecules are removed through appropriate means to obtain MIPs with the conformationally predetermined and specific recognition of the template molecules [17][18][19][20]. MIPs have been widely used in sample pre-treatment, chromatographic separations, sensors, etc.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Molecularly Imprinted Polymers for Antibiotic Analysis

et al. 2023

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The abuse and residues of antibiotics have a great impact on the environment and organisms, and their determination has become very important. Due to their low contents, varieties and complex matrices, effective recognition, separation and enrichment are usually required prior to determination. Molecularly imprinted polymers (MIPs), a kind of highly selective polymer prepared via molecular imprinting technology (MIT), are used widely in the analytical detection of antibiotics, as adsorbents of solid-phase extraction (SPE) and as recognition elements of sensors. Herein, recent advances in MIPs for antibiotic residue analysis are reviewed. Firstly, several new preparation techniques of MIPs for detecting antibiotics are briefly introduced, including surface imprinting, nanoimprinting, living/controlled radical polymerization, and multi-template imprinting, multi-functional monomer imprinting and dummy template imprinting. Secondly, several SPE modes based on MIPs are summarized, namely packed SPE, magnetic SPE, dispersive SPE, matrix solid-phase dispersive extraction, solid-phase microextraction, stir-bar sorptive extraction and pipette-tip SPE. Thirdly, the basic principles of MIP-based sensors and three sensing modes, including electrochemical sensing, optical sensing and mass sensing, are also outlined. Fourthly, the research progress on molecularly imprinted SPEs (MISPEs) and MIP-based electrochemical/optical/mass sensors for the detection of various antibiotic residues in environmental and food samples since 2018 are comprehensively reviewed, including sulfonamides, quinolones, β-lactams and so on. Finally, the preparation and application prospects of MIPs for detecting antibiotics are outlined.

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Preparation and Characterization of Protein Molecularly Imprinted Poly (Ionic Liquid)/Calcium Alginate Composite Cryogel Membrane with High Mechanical Strength for the Separation of Bovine Serum Albumin

Cited by 8 publications

References 40 publications

Molecularly Imprinted Polymer with High Capacity and Selectivity for Separation of Bovine Serum Albumin

Molecularly Imprinted Polymer with High Capacity and Selectivity for Separation of Bovine Serum Albumin

Enhancing Cytochrome C Recognition and Adsorption through Epitope-Imprinted Mesoporous Silica with a Tailored Pore Size

Recent Advances in Molecularly Imprinted Polymers for Antibiotic Analysis

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