Preparation and characterization of Protein A-immobilized PVDF and PES membranes

Akashi, Naohisa; Kuroda, Shin–ichi

doi:10.3144/expresspolymlett.2015.2

Cited by 14 publications

(13 citation statements)

References 30 publications

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“…In addition to low-pressure plasmas, atmospheric pressure argon plasmas were used for treating PVDF materials [ 14 , 15 ]. An extensive paper was published by Akashi and Kuroda [ 14 ].…”

Section: Modification Of Pvdf Surface By Exposure To Argon Plasmamentioning

confidence: 99%

Non-Equilibrium Plasma Methods for Tailoring Surface Properties of Polyvinylidene Fluoride: Review and Challenges

et al. 2021

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Modification and functionalization of polymer surface properties is desired in numerous applications, and a standard technique is a treatment with non-equilibrium gaseous plasma. Fluorinated polymers exhibit specific properties and are regarded as difficult to functionalize with polar functional groups. Plasma methods for functionalization of polyvinylidene fluoride (PVDF) are reviewed and different mechanisms involved in the surface modification are presented and explained by the interaction of various reactive species and far ultraviolet radiation. Most authors used argon plasma but reported various results. The discrepancy between the reported results is explained by peculiarities of the experimental systems and illustrated by three mechanisms. More versatile reaction mechanisms were reported by authors who used oxygen plasma for surface modification of PVDF, while plasma sustained in other gases was rarely used. The results reported by various authors are analyzed, and correlations are drawn where feasible. The processing parameters reported by different authors were the gas pressure and purity, the discharge configuration and power, while the surface finish was predominantly determined by X-ray photoelectron spectroscopy (XPS) and static water contact angle (WCA). A reasonably good correlation was found between the surface wettability as probed by WCA and the oxygen concentration as probed by XPS, but there is hardly any correlation between the discharge parameters and the wettability.

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“…In addition to low-pressure plasmas, atmospheric pressure argon plasmas were used for treating PVDF materials [ 14 , 15 ]. An extensive paper was published by Akashi and Kuroda [ 14 ].…”

Section: Modification Of Pvdf Surface By Exposure To Argon Plasmamentioning

confidence: 99%

Non-Equilibrium Plasma Methods for Tailoring Surface Properties of Polyvinylidene Fluoride: Review and Challenges

et al. 2021

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“…Organic fouling causes the blocking of the membrane pores by organic substances such as proteins, amino acids, carbohydrates, etc. [7][8][9]. Biofouling relates to the microbial adhesion and growth on the membrane surface, leading to the formation of a biofilm layer.…”

mentioning

confidence: 99%

Novel electrospun polyvinylidene fluoride-graphene oxide-silver nanocomposite membranes with protein and bacterial antifouling characteristics

Liu¹,

Shen²,

Liao³

et al. 2018

Express Polym. Lett.

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“…Figure represent the carbon (C 1 s), oxygen (O 1 s) and sulfur (S2p) regions in the high resolution spectrum XPS analysis of virgin PES nanofibrous membrane, Ab immobilized PES nanofibrous membrane through hydrophobic interactions and Ab immobilized PES nanofibrous membrane through EDC/NHS. As it is depicted in Figure the C1s of the virgin PES nanofibrous membrane, three distinct peaks at BEs of 284.6 eV due to aromatic C‐C, 285.3 eV due to C‐SO2‐C and 286.3 eV due to C‐O‐C . Second, the O1s high resolution spectrum of the virgin PES nanofibrous membrane can be deconvoluted into three peaks at BEs of 531.6 eV due to the sulfonyl group, C‐SO2‐C, 532.3 eV due to oxygen in the hydroxy group, R‐OH likely because of preservative that manufacturer added to it: and 533.3 eV due to C‐O‐C.…”

Section: Resultsmentioning

confidence: 91%

“…Surprisingly, it was observed that antibody immobilized PES membrane through EDC/NHS reveals larger increases in oxygen%, and declines in sulfur% suggesting that higher amount of could immobilized on the top surface of nanofibrous membrane trough EDC/NHS. Figure 9 the C1s of the virgin PES nanofibrous membrane, three distinct peaks at BEs of 284.6 eV due to aromatic C-C, 285.3 eV due to C-SO2-C and 286.3 eV due to C-O-C. 29 Second, the O1s high resolution spectrum of the virgin PES nanofibrous membrane can be deconvoluted into three peaks at BEs of 531.6 eV due to the sulfonyl group, C-SO2-C, 532.3 eV due to oxygen in the hydroxy group, R-OH likely because of preservative that manufacturer added to it: and 533.3 eV due to C-O-C.…”

Section: Resultsmentioning

confidence: 99%

Electrospun polyethersolfone nanofibrous membrane as novel platform for protein immobilization in microfluidic systems

et al. 2017

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In the present study, the feasibility of electrospun polyethersolfone (PES) nanofibrous membrane as the solid substrate for microfluidic based immunoassays to enhance the density of immobilized antibody on the surface of membrane was assessed. Conversely, the efficacy of antibody immobilization was compared by two different strategies as 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) coupling chemistry and hydrophobic interaction. Compared to conventional immunoassays carried out in plates or gels, microfluidic based immunoassays grant a lot of advantages such as a consumption of little samples and reagents, shorter analysis time, and higher efficiency. Therefore, microfluidic immunoassays can be efficiently used as a point-of-care device in medical diagnosis. Surprisingly, we found the increase of specific surface areas of the microfluidic channels improve density of immobilized proteins and leads to higher signal strength. Anti-staphylococcus enterotoxin B (anti-SEB) was used as an analyte model to demonstrate the utility of our proposed platform. Fluorescent microscopy, Fourier transform infrared spectroscopic (FTIR), gas adsorption, contact angle, X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), Uv-Vis spectrophotometer and atomic force microscopy (AFM) techniques were used to assess the efficacy of antibody immobilization on the surface. To understand dominant mechanism of protein immobilization, zeta potential measurement was also carried out and it was found electrostatic attraction play significant role in antibody immobilization running into micro- channels containing through EDC/NHS. Moreover, incorporation of nanofibrous membrane causes significant improvement in the signal detection of microfluidic based immunoassay. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1108-1120, 2018.

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Preparation and characterization of Protein A-immobilized PVDF and PES membranes

Cited by 14 publications

References 30 publications

Non-Equilibrium Plasma Methods for Tailoring Surface Properties of Polyvinylidene Fluoride: Review and Challenges

Non-Equilibrium Plasma Methods for Tailoring Surface Properties of Polyvinylidene Fluoride: Review and Challenges

Novel electrospun polyvinylidene fluoride-graphene oxide-silver nanocomposite membranes with protein and bacterial antifouling characteristics

Electrospun polyethersolfone nanofibrous membrane as novel platform for protein immobilization in microfluidic systems

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