Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice

Abstract

Insoluble dietary fiber extracted from soybean residue had a loose and porous structure, polysaccharide groups, and typical crystalline cellulose I structure. It played a role in regulating lipid metabolism by promoting fatty acid oxidation and inhibiting lipid accumulation.

Changes of High-Purity Insoluble Fiber from Soybean Dregs (Okara) after Being Fermented by Colonic Flora and Its Adsorption Capacity

“…To solve the problem, we prepared high purity insoluble dietary fiber from okara (Okara-HPIDF) by enzymatic method, which purity could exceed 90% [16], and proved that it had potential bioactivities. Meanwhile, we proved that Okara-HPIDF had certain functions, such as improving lipid metabolism and so on [17,18]. In the process of studying its relationship with the changes of the colonic environment, we found that Okara-HPIDF could significantly change the composition of colonic flora [18].…”

“…After identification, its main components include: dietary fiber ≥ 60%, protein ≤ 26%, moisture ≤ 10%, ash ≤ 6%. HPIDF from okara was prepared by complex enzymatic method of LPDF [17]. The purity of HPIDF was 91.25% (according to the enzymatic-gravimetric method described in Chinese national standard GB 5009.88-2014).…”

Oat β-glucan and l-arabinose synergistically ameliorate glucose uptake in insulin-resistant HepG2 cells and exert anti-diabetic activity via activation of the PI3K/AKT pathway in db/db mice

“…RNA extraction and quantitative real-time PCR (qRT-PCR) analysis qRT-PCR was performed according to a previous method. 30 In brief, total RNA was isolated from the frozen liver tissues using Trizol reagent and reverse-transcribed into cDNA with a cDNA Synthesis Kit. Next, mRNA expression was quantified by qRT-PCR using the SYBR Premix Ex Taq TM mix on Biometra TProfessional PCR (Jena, German).…”

Insoluble Dietary Fiber from Okara Combined with Intermittent Fasting Treatment Synergistically Confers Antiobesity Effects by Regulating Gut Microbiota and Its Metabolites

“…Our earlier investigation suggested that high-purity IDF from soybean residue (okara) (HPSIDF) could ameliorate HFD-induced lipid metabolism disorders, but its potential mechanism remains unclear. 18 Aside from dietary supplementation, intermittent fasting (IF) has gained a lot of interest as an emerging dietary strategy against obesity. IF is comprised of two basic strategies: time-restricted feeding and alternate-day fasting.…”