Preparation and cytotoxicity evaluation of hydrogels based on poly(trimethylene carbonate) and carboxyalkyl chitosans for regenerative medicine applications

Zemlyakova, E. O.; Samokhin, A. G.; Korel, Anastasya V.; Kuznetsov, V. A.; Ткаченко, В. О.; Нестеров, Д. В.; Filatova, Svetlana B.; Пахомов, И. А.; Pavlov, V. V.; Larionov, P. M.; Пестов, А. В.

doi:10.1016/j.matpr.2019.12.182

Cited by 1 publication

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“…The presence of carboxyalkyl groups in the chitosan molecule enhances polymer affinity to the cell substrates [ 19 , 20 ]. In our previous study [ 21 ], CEC demonstrated one of the best cytotoxicity results in the human fibroblast cell culture and did not cause cell death. Based on these results, we decided to characterize CEC’s structure and swelling performance, along with its phage delivery properties, for its use as a potential carrier for delivering phages in an in vitro gastrointestinal tract (GIT) pH environment model simulation of a laboratory animal (mouse), in two pH-fixed medium (alkaline or acidic) variants, and as a candidate gel for biomedical application.…”

Section: Introductionmentioning

confidence: 99%

A Carboxyethylchitosan Gel Cross-Linked with Glutaraldehyde as a Candidate Carrier for Biomedical Applications

Korel,

Samokhin,

Zemlyakova

et al. 2023

Gels

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To date, few publications describe CEC’s properties and possible applications—thus, further evaluation of these properties is a point of interest. The present in vitro model study aimed to evaluate a carboxyethylchitosan (CEC) gel with a degree of substitution of 1, cross-linked with glutaraldehyde at a polymer:aldehyde molar ratio of 10:1, as a potential carrier for delivering bacteriophages to various pH-fixed media (acidic, alkaline), and including gastrointestinal tract (GIT) variable medium. A quantitative analysis of bacteriophages released from the gel was performed using photon correlation spectrophotometry, and phage activity after emission into medium was evaluated using the spot test. The results showed that the CEC gel’s maximum swelling ratios were at a nearly neutral alkaline pH. Increasing temperature enhances the swelling ratio of the gel independent from pH, up to 1127% at 37 °C and alkaline pH. The UV and photon correlation spectrophotometry showed equal gel release kinetics in both fixed media with acidic (pH = 2.2) and alkaline (pH = 7.4) pH environments at 37 °C, with the maximum release within two hours. However, phage lytic activity in the spot test during this simulation was absent. At the same time, we obtained an opaque phage lytic activity in the alkaline pH-fixed medium for at least three hours. Phages released from the tested CEC gel in different pHs suggest that this gel could be used for applications that require fast release at the treatment site both in acidic and alkaline pH. Such treatment sites could be a wound or even soil with mild acidic or alkaline pH. However, such CEC gel is not suitable as a delivery system to the GIT because of possible transported acid-sensitive agent (such as phages) release and destruction already in the stomach.

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Section: Introductionmentioning

confidence: 99%