Preparation and Detection of the Resolution and Number of Theoretical Plates for High Purity Steviosides

Abstract

Macroporous resin was the most common method for the separation and purification of steviosides. But the purity was only 90% influenced by less selectivity of the resin. And Rebaudioside A could not be separated from other steviosides at the same time. Purity of total glycosides could be up to 99% and Rebaudioside A could be enriched efficiently by section desorption after one-dimensional chromatography of macroprous resins. The content of Rebaudioside A increased from 39.46% to 63.70% gradually in each desorption solution of the 4 resin columns. Moreover, the resolution between Rebaudioside A, Rebaudioside C and stevioside, and the number of theoretical plates for them were detected meanwhile. These results could provide theoretical foundation for the separation of high purity steviosides and enrichment of Rebaudioside A.