Preparation and enzymatic hydrolysis of nanoparticles made from single xyloglucan polysaccharide chain

Mkedder, Ilham; Travelet, Christophe; Durand-Terrasson, Amandine; Halila, Sami; Dubreuil, Frédéric; Borsali, Rédouane

doi:10.1016/j.carbpol.2013.02.001

Cited by 24 publications

(8 citation statements)

References 26 publications

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“…For this material the chain width was 1.06–1.81 nm for ripe soft and 1.65–5.22 nm for ripe crisp fruit. Mkedder et al [ 42 ] described tamarind xyloglucan nanoparticles prepared in NaNO 2 , deposited on hydrophilic silicon substrate and incubated with 1,4-β-endoglucanase enzyme that their diameter is from 5 to 12 nm. Molecular dynamic simulations estimated dimensions of XG monomers unit as 1.41-1.49 nm (length) and 0.62–0.75 nm (width) [ 40 ], depending on the number of side-chains.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Structure and Assembly of Xyloglucan from Tamarind Seed (Tamarindus indica L.) with Atomic Force Microscopy

et al. 2015

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The role of xyloglucan (XG) in the cell wall of plants and its technological usability depends on several factors, pertaining to molecular structure. Therefore, the goal of this study was to evaluate the nano-structure and self-assembly of XG by atomic force microscopy (AFM). As the model, a non-modified xyloglucan from a tamarind seed (Tamarindus indica L.) was used. Samples were minimally processed, i.e., treated with low-power ultrasound and studied on the surface of mica in ambient butanol. AFM topographic images revealed rod-like nanomolecules of xyloglucan with a mean height of 2.3 ± 0.5 nm and mean length of 640 ± 360 nm. The AFM study also showed that XG chains possessed a helical structure with a period of 115.8 ± 29.2 nm. This study showed possible-bending of molecules with a mean angle of 127.8 ± 25.6°. The xyloglucan molecules were able to aggregate as cross-like and a parallel like assemblies, and possibly as rope-like structures. The self-assembled bundles of xyloglucan chains were often complexed at an angle of 114.2 ± 36.3°.

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Section: Resultsmentioning

confidence: 99%

Evaluation of Structure and Assembly of Xyloglucan from Tamarind Seed (Tamarindus indica L.) with Atomic Force Microscopy

et al. 2015

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“…They are a source of biopolymers generally non-toxic, biodegradable, and biocompatible, sometimes exhibiting properties of biorecognition [1]. The polysaccharides have a wide range of chemical structure and physiochemical properties that cannot be easily reproduced synthetically.…”

Section: Introductionmentioning

confidence: 99%

Structure and rheological properties of a xyloglucan extracted from Hymenaea courbaril var. courbaril seeds

Arruda

Albuquerque

Santos

et al. 2015

International Journal of Biological Macromolecules

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Hymenaea courbaril var courbaril seed xyloglucan was efficiently extracted with 0.1M NaCl, followed by ethanol precipitation (yield=72±5% w/w). Its amorphous structure was identified by the pattern of X-ray diffraction. The monosaccharide composition was determined by GC/MS analysis of the alditol acetates and showed the occurrence of glucose:xylose:galactose:arabinose (40:34:20:6). One-(1D) and two-dimensional-(2D) NMR spectra confirmed a central backbone composed by 4-linked β-glucose units partially branched at position 6 with non-reducing terminal units of α-xylose or β-galactose-(1→2)-α-xylose disaccharides. The xyloglucan solution was evaluated by dynamic light scattering and presents a polydisperse and practically neutral profile, and at 0.5 and 1.0% (w/v) the solutions behave as a viscoelastic fluid. The polysaccharide did not show significant antibacterial or hemolytic activities. Overall our results indicate that xyloglucan from H. courbaril is a promising polysaccharide for food and pharmaceutical industries.

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“…Natural polymers inherit numerous advantages including natural abundance, relative ease of isolation, and room for chemical modification to meet varying technological needs. In addition, these polymers undergo enzymatic and/or hydrolytic degradation in biologic environments releasing body friendly degradation byproducts . Polysaccharides are an important class of biomaterials with significant research interest for a variety of drug delivery and tissue regeneration applications because of their assured biocompatibility and bioactivity.…”

Section: Introductionmentioning

confidence: 99%

Polysaccharide biomaterials for drug delivery and regenerative engineering

Shelke

James

Laurencin

et al. 2014

Polymers for Advanced Techs

255

148

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Polymers derived from plant (polysaccharides) and animal (proteins) kingdoms have been widely used for a variety of biomedical applications including drug delivery and tissue regeneration. These polymers due to their biochemical similarity with human extracellular matrix components are readily recognized and accepted by the body. Natural polymers inherit numerous advantages including natural abundance, relative ease of isolation, and room for chemical modification to meet varying technological needs. In addition, these polymers undergo enzymatic and/or hydrolytic degradation in biologic environments into non-toxic degradation byproducts. Polysaccharides (carbohydrates) are often isolated and purified from renewable sources including plants, animals, and microorganisms. Majority of these polymers are found in the extracellular matrix components of organisms and participate in inter and intracellular cell signaling and contribute to their growth. All these features offer polysaccharide-based biomaterials much desired biological recognition, biocompatibility, and bioactivity. In spite of many merits as biomaterials, these polysaccharides suffer from drawbacks including variations in material properties based on source, microbial contamination, uncontrolled water uptake, poor mechanical strength, and unpredictable degradation patterns. These inconsistencies have limited the usage of polysaccharides and biomedical application related technology development. Many of these polysaccharides have been chemically modified to achieve consistent physicochemical properties including mechanical stability, degradation, and bioactivity and processed into microparticles, hydrogels, and 3D porous structures for tissue regeneration applications. Presence of multiple functionalities on the polymer backbone allows easy structure modifications for the required application. The current article focuses on the application of polysaccharide-based materials in regenerative engineering and delivery.

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Preparation and enzymatic hydrolysis of nanoparticles made from single xyloglucan polysaccharide chain

Cited by 24 publications

References 26 publications

Evaluation of Structure and Assembly of Xyloglucan from Tamarind Seed (Tamarindus indica L.) with Atomic Force Microscopy

Evaluation of Structure and Assembly of Xyloglucan from Tamarind Seed (Tamarindus indica L.) with Atomic Force Microscopy

Structure and rheological properties of a xyloglucan extracted from Hymenaea courbaril var. courbaril seeds

Polysaccharide biomaterials for drug delivery and regenerative engineering

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