Preparation and evaluation of anti-SARS coronavirus IgY from yolks of immunized SPF chickens

Fu, Chao-Yang; Huang, He; Wang, Xiaomei; Liu, Yonggang; Wang, Zhiguo; Cui, Shangjin; Gao, Honglei; Li, Zan; Li, Jingpeng; Kong, Xiangang

doi:10.1016/j.jviromet.2005.10.027

Cited by 58 publications

(56 citation statements)

References 4 publications

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“…It could be lyophilized, reconstituted without loss of activity, and maintain good thermal stability. 143 Other theoretic treatments include treatment of SARS ARDS by blocking the pulmonary renin-angiotensin system or treatment with ACE-2. 37,38 Uncontrolled trials suggest that IFN alfacon-1 (a synthetic interferon) with steroids, protease inhibitors with ribavirin, or convalescent plasma with neutralizing antibody may be useful for treatment.…”

Section: Eye Of Newt and Toe Of Frog Wool Of Bat And Tongue Of Dogmentioning

confidence: 99%

Severe Acute Respiratory Syndrome (SARS)

Cleri

Ricketti

Vernaleo

2010

Infectious Disease Clinics of North America

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Section: Eye Of Newt and Toe Of Frog Wool Of Bat And Tongue Of Dogmentioning

confidence: 99%

Severe Acute Respiratory Syndrome (SARS)

Cleri

Ricketti

Vernaleo

2010

Infectious Disease Clinics of North America

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“…However, chicken egg is also considered as a major source of antibodies. In the past few years, many kinds of specific antibodies have been yielded successfully from eggs of immunised hens, which include anti-HIV-1 and anti-SARS coronavirus antibodies (Devi, Bai, Lal, Umashankar, & Krishnan, 2002;Fu et al, 2006;McLaren, Prosser, Grieve, & Brissenko, 1994;Ruan, Ma, He, Meng, & Zhu, 2005;Soler, Barquet, & Lucio, 1998). Presently, IgY has been used extensively for prevention and treatment of various infections in animals and humans with mixed success (Hamada & Kodama, 1996).…”

Section: Introductionmentioning

confidence: 99%

A new membrane based process to isolate immunoglobulin from chicken egg yolk

Liu

Yang

et al. 2010

Food Chemistry

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a b s t r a c tAlthough the importance of eggs as a source of specific antibodies has been well recognised, the generation of egg yolk immunoglobulins (IgY) is rarely chosen due to the peculiar composition of egg yolk and the lack of specific affinity ligands. In this work, we report a novel membrane based two-stage ultrafiltration process to isolate IgY from egg yolk. The effects of solution pH, ionic strength, stirring speed and permeate flux on the transmission of proteins were quantified using the pulsed sample injection technique and parameter scanning ultrafiltration. Under optimised conditions, the purity of immunoglobulin obtained was greater than 93% after the two-stage ultrafiltration process and the recovery of immunoglobulin from the feedstock was close to 87%. The resulting immunoglobulin product was then analysed by Isoelectric Focusing (IEF), SDS-PAGE and Circular Dichroism (CD), to confirm its isoelectric point, molecular weight and molecular secondary structure.

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“…The oral administration of specific IgYs has been shown to be an alternative method for the treatment and prevention of gastrointestinal pathogens including Salmonella SPP. (Chalghoumi et al, 2009;Pavic et al, 2012), Escherichia coli (Sunwoo et al, 2006), Bovine coronavirus (Fu et al, 2006), Bovine rotavirus (Vega et al, 2011), Pseudomonas Staphylococcus (Wang et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Production, purification, and evaluation of quail immunoglobulin Y against Salmonella typhimurium and Salmonella enteritidis

Esmailnejad

Abdi-Hachesoo

Nasab

et al. 2019

Molecular Immunology

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A B S T R A C TSalmonella species have been the major foodborne problems in food production systems, with Salmonella enterica serovars typhimurium (S. typhimurium) and enteritidis (S. enteritidis) being among the more common isolates. The oral administration of chicken egg yolk specific antibodies (IgYs) has been established as an efficient alternative for treatment and prevention of gastrointestinal pathogens including Salmonella. The present study was aimed to investigate the possible production of specific IgYs against Salmonella typhimurium and Salmonella enteritidis in quail egg yolks. Salmonella spp.-free female Japanese quails (Coturnix coturnix japonica) were intramuscularly immunized with formalin or heat-inactivated Salmonella immunogens (1.0 × 10 9 CFU/mL) emulsified with Freund adjuvants. Egg yolk IgYs were purified using ammonium sulfate precipitation method. Anti-Salmonella IgYs titer and specificity were determined using enzyme-linked immunosorbent assay (ELISA) and western blot analysis. Salmonella specific IgYs detected in the immunized quails were significantly higher than those of the control group, which confirmed the immunization procedure. Specific IgYs against S. typhimurium and S. enteritidis were identified in both groups immunized with heat or formalin-inactivated immunogens. However, formalin-inactivated immunogens induced relatively higher immune responses over the heat-inactivated ones. Quail anti-Salmonella IgYs showed a high specificity to their corresponding immunogens, with moderate crossreactivity to other members of Enterobacteriaceae family. Quail can be regarded as a valuable and inexpensive source for producing large-scale of specific antibodies that can be used for immunodiagnostic and immunotherapeutic purposes.

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Preparation and evaluation of anti-SARS coronavirus IgY from yolks of immunized SPF chickens

Cited by 58 publications

References 4 publications

Severe Acute Respiratory Syndrome (SARS)

Severe Acute Respiratory Syndrome (SARS)

A new membrane based process to isolate immunoglobulin from chicken egg yolk

Production, purification, and evaluation of quail immunoglobulin Y against Salmonella typhimurium and Salmonella enteritidis

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