Preparation and evaluation of chitosan-calcium-gellan gum beads for controlled release of protein

Yang, Fei; Xia, Shuqin; Tan, Chen; Zhang, Xiaoming

doi:10.1007/s00217-013-2021-y

Cited by 82 publications

(23 citation statements)

References 29 publications

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“…The shifting of Amide I and Amide II absorption band from standard CHI to smaller wavenumbers in the case of membrane C (1646 cm −1 and 1564 cm −1 , respectively) also demonstrated the interaction with carboxyl groups of GEL [31,33]. Furthermore, the band corresponding to the C–O–C bond, which in standard CHI occurred at 1150 cm −1 , in the IR spectrum of the membrane C, this band appeared at 1155 cm −1 , but at a very low intensity, demonstrating cross-linking with GA [12].…”

Section: Resultsmentioning

confidence: 99%

Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications

et al. 2018

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In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP). This technology involved metal ions incorporation to develop new biopolymeric supports. The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM). The inductively coupled plasma mass spectrometry (ICP-MS) evidenced the metal ion release in time. Moreover, the effect of Mg2+ on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied. Immobilizing ALP into the chitosan membranes composition followed by the incorporation of Mg2+ led to polymeric supports with enhanced cellular viability when comparing to chitosan-based membranes without Mg2+. The results obtained evidenced promising performance in biomedical applications for the new biopolymeric supports that are based on chitosan, ALP, and metal ions.

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Section: Resultsmentioning

confidence: 99%

Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications

et al. 2018

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“…A pH of 6.5 strikes a balance between EDC's peak reactivity at pH ~4.5, and that of the terminal amine, which is strongly activated at pH 8-9 24 . The GG pKa value of 3.06 implies that a pH of 6.5 promotes dissociation in over 99.9% of available carboxylate residues 37 . However, despite the similar pH value (6.7) the sodium acetate buffer impeded RGD peptide coupling, possibly due to de-activation of EDC by nucleophilic attack from acetate ions 38 , underscoring the importance of appropriate buffer selection.…”

Section: Peptide Conjugation To Purified Ggmentioning

confidence: 99%

Peptide modification of purified gellan gum

et al. 2015

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Gellan gum (GG) is an anionic polysaccharide with potential as a biopolymer for additive manufacturing (3D-bioprinting) and tissue engineering. Previous studies have shown GG to be highly cytocompatible, but lacking specific attachment sites required for anchorage-dependent cells. In this work, we modify purified-GG polymer with a short peptide containing the arginine-glycine-aspartic acid (RGD) sequence that is known to enhance integrin-mediated cell attachment. Radiolabelling of the peptide was used in optimisation of the conjugation procedure to achieve an overall efficiency of 40%. The purification of divalent cations from commercial GG samples was found to be critical for successful conjugation. Rheological studies revealed that the peptide coupling did not prevent gelation behaviour. C2C12 cells showed improved attachment on the surface of and encapsulated within RGD-GG hydrogels, differentiating to multinucleated myofibers after 5-7 days. PC12 cells showed minimal interactions with both GG and RGD-GG, with formation of cell clusters and impedance of terminal differentiation and neurite extension. Gellan gum (GG) is an anionic polysaccharide with potential as a biopolymer for additive manufacturing (3D-bioprinting) and tissue engineering. Previous studies have shown GG to be highly cytocompatible, but lacking specific attachment sites required for anchorage-dependent cells. In this work, we modify purified-GG polymer with a short peptide containing the arginineglycine-aspartic acid (RGD) sequence that is known to enhance integrin-mediated cell attachment. Radiolabelling of the peptide was used in optimisation of the conjugation procedure to achieve an overall efficiency of 40%. The purification of divalent cations from commercial GG samples was found to be critical for successful conjugation. Rheological studies revealed that the peptide coupling did not prevent gelation behaviour. C2C12 cells showed improved attachment on the surface of and encapsulated within RGD-GG hydrogels, differentiating to multinucleated myofibers after 5-7 days. PC12 cells showed minimal interactions with both GG and RGD-GG, with formation of cell clusters and impedance of terminal differentiation and neurite extension.

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“…It means that the release occurred by the Fickian diffusion through the swelling matrix, and it was faster for the gels with higher calcium concentration. Yang et al [30] studied the release of egg white albumen from chitosan-calcium-gellan gum beads and fitted it to the Korsmeyer-Peppas model. In this research the release also occurred by the diffusion process as the value of the coefficient "n" was 0.43.…”

Section: Digestion Of the Gels In An Artificial Stomachmentioning

confidence: 99%

New controlled release material: aerated egg white gels induced by calcium ions

Tomczyńska‐Mleko

Handa²,

Wesołowska–Trojanowska

et al. 2016

Eur Food Res Technol

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by Fickian diffusion through the swelling matrix and it was faster for the gels with higher concentration of calcium. An increase in calcium concentration caused an increase in surface roughness of the aerated gels. There were linear correlations between the quadratic mean of the surface roughness and "n" coefficient from the Ritger and Peppas equation and between the maximum roughness size and "n". The increased calcium concentration made the gel microstructure more particulate and the surface rougher, which enabled faster proteolysis of the gel in an artificial stomach and faster diffusion of calcium ions.Keywords Aerated · Egg white · Gel · Release · Microstructure · Surface properties · Rheology Abstract The aim of the research was to use low-mineral egg white albumin isolate to obtain aerated calcium ioninduced gels and to investigate their aptitude as matrices for active ingredients release. Aerated gels were prepared by adding calcium ions to pre-heated protein dispersions with simultaneous aeration. 20 mM calcium concentration was an optimal concentration at which the maximum viscosity and hardness of the gels were found. For higher concentrations of calcium ions (25-30 mM), aerated gels were characterized by lower values of the moduli and tangent of the phase angle was larger for aerated gels which indicated more viscous character than that of non-aerated gels. Increased ions concentration causes higher aggregation of protein matrix and less smooth microstructure of the interface between the gel and air. An increase in calcium concentration from 5 to 30 mM caused an increase in an average bubble size. The release of calcium ions from aerated gels was measured in an artificial stomach. It occurred * Stanisław Mleko

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Preparation and evaluation of chitosan-calcium-gellan gum beads for controlled release of protein

Cited by 82 publications

References 29 publications

Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications

Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications

Peptide modification of purified gellan gum

New controlled release material: aerated egg white gels induced by calcium ions

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