Preparation and evaluation of crocin loaded in nanoniosomes and their effects on ischemia–reperfusion injuries in rat kidney

Naderi, Reyhaneh; Pardakhty, Abbas; Abbasi, Mohammad Farajli; Ranjbar, Mehdi; Iranpour, Maryam

doi:10.1038/s41598-021-02073-w

Cited by 13 publications

(4 citation statements)

References 27 publications

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“…Additionally, the small size of NPs enables more effective interaction with tumor cells. Previous studies have reported the anticancer properties of crocin, as well as the effects of Nanosystems carrying crocin 21 , 44 . However, no research has been conducted on cellulose nanocarriers modified with CTAB as a cationic surfactant and FA to deliver crocin to cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Folic acid-modified nanocrystalline cellulose for enhanced delivery and anti-cancer effects of crocin

Soltani,

Farhadi,

Rajabi

et al. 2024

Sci Rep

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Crocin is a carotenoid compound in saffron with anti-cancer properties. However, its therapeutic application is limited by its low absorption, bioavailability, and stability, which can be overcome through nanocarrier delivery systems. This study used surface-modified Nano-crystalline cellulose (NCC) to deliver crocin to cancer cells. NCC modified with CTAB were loaded with crocin and then conjugated with folic acid (NCF-CR-NPs). The synthesized nanoparticles (NPs) were characterized using FTIR, XRD, DLS, and FESEM. The crystallinity index of NCC was 66.64%, higher than microcrystalline cellulose (61.4%). The crocin loading and encapsulation efficiency in NCF-CR-NPs were evaluated. Toxicity testing by MTT assay showed that NCF-CR-NPs had higher toxicity against various cancer cell lines, including colon cancer HT-29 cells (IC50 ~ 11.6 μg/ml), compared to free crocin. Fluorescent staining, flow cytometry, and molecular analysis confirmed that NCF-CR-NPs induced apoptosis in HT-29 cells by increasing p53 and caspase 8 expression. The antioxidant capacity of NCF-CR-NPs was also evaluated using ABTS and DPPH radical scavenging assays. NCF-CR-NPs exhibited high free radical scavenging ability, with an IC50 of ~ 46.5 μg/ml for ABTS. In conclusion, this study demonstrates the potential of NCF-CR-NPs to deliver crocin to cancer cells effectively. The NPs exhibited enhanced anti-cancer and antioxidant activities compared to free crocin, making them a promising nanocarrier system for crocin-based cancer therapy.

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Section: Discussionmentioning

confidence: 99%

Folic acid-modified nanocrystalline cellulose for enhanced delivery and anti-cancer effects of crocin

Soltani,

Farhadi,

Rajabi

et al. 2024

Sci Rep

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“…Studies have demonstrated the protective effect of Saffron on oxidative stress in different tissues such as abdominal organs, kidney, testis, and endothelial cells [ 21 , 41 – 46 ]. Crocin, safranal, carotene and crocetin as the most abundant constituents of Saffron stigmas, represent antioxidant effects.…”

Section: Discussionmentioning

confidence: 99%

“…Crocin, safranal, carotene and crocetin as the most abundant constituents of Saffron stigmas, represent antioxidant effects. Noticeably, Saffron extract is mentioned as a potent anti-oxidant with high ability to scavenge ROS, especially superoxide anions and hydrogen peroxide (H 2 O 2 ) resulting in inhibitory effect on lipid peroxidation [ 22 , 29 , 32 , 41 ]. Also, Saffron is suggested in autoimmune encephalomyelitis, and cerebral ischemia [ 23 , 47 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

The Novel Role of Crocus sativus L. in Enhancing Skin Flap Survival by Affecting Apoptosis Independent of mTOR: A Data-Virtualized Study

et al. 2022

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Background Despite the improvements to enhance skin flap viability, the effects of ischemia-reperfusion (IR), oxidative stress, necrosis, and apoptosis are still challenging. Crocus sativus L. (Saffron) is highly noticeable due to its tissue-protective and antioxidant properties. So, we aimed to investigate its effects on skin flap viability, oxidative stress, apoptosis markers, histopathological changes, and mTOR/p-mTOR expression. Materials and Methods 40 Sprauge-Dawley rats, weighting 200–240 g, were divided into four groups including: (1) Sham (8 × 3 cm skin cut, without elevation); (2) Flap Surgery (8 × 3 cm skin flap with elevation from its bed); (3) Saffron 40 mg/kg + Flap Surgery; and (4) Saffron 80 mg/kg + Flap Surgery. Saffron was administrated orally for 7 days. At day 7, flap necrosis percentage, histopathological changes, malondialdehyde level, Myeloperoxidase and superoxide dismutase activity, Bax, Bcl-2, mTOR, and p-mTOR expression were measured. Protein expressions were controlled by β -Actin. Results Saffron administration decreased flap necrosis percentage ( p < 0.01), which was not dose-dependent. Treatment groups showed significant histological healing signs (Neovascularization, Fibroblast migration, Epithelialization, and Epithelialization thickness), decreased MDA content ( p < 0.01), increased SOD ( p < 0.01) and decreased MPO activity ( p < 0.01). Bax and Bcl-2 expression, decreased and increased respectively in treated groups ( p < 0.0001). mTOR and p-mTOR expression were not changed significantly in Saffron treated groups. Conclusion Saffron could increase skin flap viability, alleviate necrosis, decrease oxidative stress and decrease apoptotic cell death, after skin flap surgery, but it acts independent of the mTOR pathway. So, Saffron could potentially be used clinically to enhance skin flap viability. No Level Assigned This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266. https://www.springer.com/00266 Supplementary Information The online version contains supplementary material available at 10.1007/s00266-022-03048-6.

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“…In-vitro drug release: The in-vitro drug release study is carried out in two medium: Phosphate buffer and gastrointestinal pH medium 59,60 .…”

Section: Semmentioning

confidence: 99%

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2023

IJPSR

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Cefpodoxime proxetil is an oral antibiotic belonging to 3 rd generation Cephalosporin category. It has shown its antibiotic activity against both Gram positive and Gram-negative bacteria and has high stability in the presence of β-lactamase except Enterococci species. The drug has found its effectiveness in pediatric patients in treating respiratory tract infections. At the present time the most familiar form in which cefpodoxime proxitel is delivered is either oral tablet form or for pediatrics oral dry syrup form. The oral dry syrup form has to be administered twice or thrice a day in order to meet the required dosage regimen. Developing the sustained release formulation for cefpodoxime proxetil probably niosomal suspension can be effective in reducing the dosing frequency along with sustained release and targeted drug delivery. Niosomal suspensions are suspended form of niosomes in an aqueous phase to form liquid dosage form it is called niosomal suspension. It is a potential drug carrier for delivery of drugs orally in liquid form. The niosomal suspensions allow the targeted drug delivery with controlled/sustained release and lead to reduced dose frequency with improved bioavailability and higher therapeutic effectiveness. This could make the formulation most suitable with lesser side effects and higher patient compliance.

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Preparation and evaluation of crocin loaded in nanoniosomes and their effects on ischemia–reperfusion injuries in rat kidney

Cited by 13 publications

References 27 publications

Folic acid-modified nanocrystalline cellulose for enhanced delivery and anti-cancer effects of crocin

Folic acid-modified nanocrystalline cellulose for enhanced delivery and anti-cancer effects of crocin

The Novel Role of Crocus sativus L. in Enhancing Skin Flap Survival by Affecting Apoptosis Independent of mTOR: A Data-Virtualized Study

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