Preparation and Evaluation of Skin Wound Healing Chitosan-Based Hydrogel Membranes

Abstract

The purpose of the study was to synthesize and characterize a new form of topical membranes as chitosan-based hydrogel membranes for bacterial skin infections. The polymeric membranes were synthesized by modification in free radical solution polymerization technique. High molecular weight (HMW) chitosan polymer was cross-linked with monomer 2-acrylamido-2-methylpropane sulfonic acid (AMPS) through cross-linker N,N-methylenebisacrylamide (MBA). Mupirocin, an antibiotic, was used as model drug. The polymeric membranes were prepared in spherical form that found stable and elastic. Characterization of hydrogel membranes was performed by FTIR, SEM, DSC, TGA, swelling behavior, drug release, irritation study, and ex vivo drug permeation and deposition study. Structural and thermal studies confirmed the formation of new polymeric network with enhanced stability of hydrogel membranes. Permeation flux of drug from optimized formulation through rabbit's skin assessed by using Franz cell was up to 104.09 μg cm h. Furthermore, hydrogel membrane has significant retention of drug in skin up to 2185 μg 1.5 cm. Draize patch test confirmed the synthesized hydrogels as non-irritant to skin. The preparation of a topical membrane with improved antibacterial activity within controlled release manner is desirable for the advancement and treatment of skin diseases.

A Versatile Microencapsulation Platform for Hyaluronic Acid and Polyethylene Glycol

“…[7][8][9] In contrast, there is an abundance of alternative hydrogels that are synthetically manufactured, eliminating the variation associated with alginate purification steps. 8 Unfortunately, these hydrogels have slower gelation times than alginate, and thus are typically prepared as macroscopic structures, [10][11][12] which can result in diffusion limitations due to the low surface area. 13 Protocols have been developed for producing microspheres using alternative hydrogels such as polyethylene glycol (PEG), agarose, chitosan, or hyaluronic acid (HA), but are based on oil-emulsion techniques.…”

Double membrane based on lidocaine-coated polymyxin-alginate nanoparticles for wound healing: In vitro characterization and in vivo tissue repair

“…The presence of drugs did not significantly alter the adhesion properties of the membranes. Moreover, CS and ALG have been previously described to possess mucoadhesive properties (Ahmad et al, 2018;Guadarrama-Acevedo et al, 2019), thus excellent adhesion properties of our membranes were expected.…”

“…The mechanical properties are a parameter with significant importance since natural polymers have several limitations when compared to the synthetic ones, such as low tensile strength, elongation at break and higher flexibility (Ahmad et al, 2018;Guadarrama-Acevedo et al, 2019). Table 3 shows the mechanical properties of F3 and F4 membranes.…”

Biodegradable Polymers as Drug Delivery Systems for Bone Regeneration

“…To obtain 100% deacetylated chitosan, the partially deacetylated chitosan is filtered from the aqueous sodium hydroxide solution, thoroughly rinsed with water to remove the sodium hydroxide, and subsequently dried. Chitosan-derived biomaterials have been studied as scaffolds for nerve regeneration [64] and skin regeneration [65]. Sharifi et al [66] synthesized a scaffold consisting of nanofibers of polycaprolactone (PCL)/chitosan composite, and subsequently performed an MTT assay with MG63 cells to evaluate its cell proliferation.…”