Preparation and Evaluation of the Antibacterial Effect of Chitosan Nanoparticles Containing Ginger Extract Tailored by Central Composite Design

Farmoudeh, Ali; Shokoohi, Aynaz; Ebrahimnejad, Pedram

doi:10.34172/apb.2021.073

Cited by 15 publications

(7 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A dynamic light scattering (DLS) experiment was employed to determine the size, zeta potential, and PDI (polydispersity index) of MET-loaded NPs (Zetasizer Nano ZS system, Malvern Instruments, Worcestershire, UK) [ 27 ]. The angle of detection was 90°, the temperature was 25°C, the concentration of the specimens was 20–400 kilo counts per second, and the intensity of diffraction was 100 kilo counts per second.…”

Section: Methodsmentioning

confidence: 99%

Hyaluronic Acid‐Coated Chitosan/Gelatin Nanoparticles as a New Strategy for Topical Delivery of Metformin in Melanoma

Ebrahimnejad,

Rezaeiroshan,

Babaei

et al. 2023

BioMed Research International

Self Cite

View full text Add to dashboard Cite

Metformin is a multipotential compound for treating diabetes II and controlling hormonal acne and skin cancer. This study was designed to enhance metformin skin penetration in melanoma using nanoparticles containing biocompatible polymers. Formulations with various concentrations of chitosan, hyaluronic acid, and sodium tripolyphosphate were fabricated using an ionic gelation technique tailored by the Box-Behnken design. The optimal formulation was selected based on the smallest particle size and the highest entrapment efficiency (EE%) and used in ex vivo skin penetration study. In vitro antiproliferation activity and apoptotic effects of formulations were evaluated using MTT and flow cytometric assays, respectively. The optimized formulation had an average size, zeta potential, EE%, and polydispersity index of 329 ± 6.30 nm, 21.94 ± 0.05 mV, 64.71 ± 6.12 % , and 0.272 ± 0.010 , respectively. The release profile of the optimized formulation displayed a biphasic trend, characterized by an early burst release, continued by a slow and sustained release compared to free metformin. The ex vivo skin absorption exhibited 1142.5 ± 156.3 μg/cm2 of metformin deposited in the skin layers for the optimized formulation compared to 603.2 ± 93.1 μg/cm2 for the free metformin. Differential scanning calorimetry confirmed the deformation of the drug from the crystal structure to an amorphous state. The attenuated total reflection Fourier transform infrared results approved no chemical interaction between the drug and other ingredients of the formulations. According to the MTT assay, metformin in nanoformulation exhibited a higher cytotoxic effect against melanoma cancer cells than free metformin (IC50: 3.94 ± 0.57 mM vs. 7.63 ± 0.26 mM, respectively, P < 0.001 ). The results proved that the optimized formulation of metformin could efficiently decrease cell proliferation by promoting apoptosis, thus providing a promising strategy for melanoma therapy.

show abstract

Section: Methodsmentioning

confidence: 99%

Hyaluronic Acid‐Coated Chitosan/Gelatin Nanoparticles as a New Strategy for Topical Delivery of Metformin in Melanoma

Ebrahimnejad,

Rezaeiroshan,

Babaei

et al. 2023

BioMed Research International

Self Cite

View full text Add to dashboard Cite

show abstract

“…Chitosan nanoparticles of permethrin were synthesised by ionic gelation method [4717]. The chitosan powder was dissolved in 1% (v/v) acetic acid solution followed by 15–20 min of constant stirring on a magnetic stirrer.…”

Section: Methodsmentioning

confidence: 99%

Preparation, characterisation and comparative toxicity of nanopermethrin against Anophelesstephensi and Culexpipiens

Ebrahimnejad

Nikookar

Fazeli‐Dinan

et al. 2021

Tropical Med Int Health

Self Cite

View full text Add to dashboard Cite

Objectives To assess the effectiveness of nanopermethrin as a potential new formulation for pest and vector control. Methods Permethrin nanoparticles were prepared by the ionic gelation method and its structure and the formulations were designed using Box–Behnken statistical technique. The effect of independent variables (Chitosan/Permethrin ratio, tripolyphosphate quantity, sonication time) on the properties of nanoparticles was investigated to determine the optimal formulation. Results The size of the nanoparticles ranged from 135.27 ± 5.88 to 539.5 ± 24.01 nm and the insecticide entrapment efficiency per cent (EE%) ranged from 7.72 ± 1.36 to 63.59 ± 3.17%. Anopheles stephensi larvae were then bioassayed with the nanopermethrin and compared with the results of the bioassay with the mother molecule of permethrin using a standard WHO‐recommended mosquito larval bioassay kit. LC50 with permethrin and nanopermethrin on larvae of An. stephensi were 0.125 and 0.026 ppm showing a 4.8 times difference. The LC50 for permethrin and nanopermethrin on Culex pipiens were 0.003 and 0.00032 ppm, respectively, showing a 9.4‐fold difference. Conclusion Nanopermethrin is much more potent than its mother molecule against larvae of An. stephensi and Cx. pipiens.

show abstract

“…It has been shown that nanoparticle-based delivery technologies improve the biological activity of herbal extracts as prebiotics 62 . For instance, by specifically encouraging the growth of advantageous gut bacteria, the encapsulating of ginger extract in chitosan nanoparticles improved its stability and increased its prebiotic effect 63 . Similar to this, resveratrol's solubility and stability were increased through its encapsulation in solid lipid nanoparticles, enabling targeted administration to the colon and regulation of the gut microbiota 64 .…”

Section: Nanoparticle-based Delivery Systemsmentioning

confidence: 99%

Metal-Infused Polyphenol-enriched Phyto-fabricated Nanoparticles: an In-depth Review of their Potent Prebiotic Properties

Reddy,

Aluri,

A. R. Shashikala

2023

jmmf

View full text Add to dashboard Cite

In light of its potential health benefits, including as improved gut health and management of the gut microbiota, herbal extracts have witnessed a major increase in demand as prebiotics in recent years. Many studies have demonstrated that polyphenolrich herbal extracts can work as prebiotics by encouraging the growth of beneficial gut flora. Unfortunately, the limited solubility and stability of these herbal extracts, as well as their susceptibility to breakdown in the gastrointestinal system, can limited their bioavailability and efficiency. To address these limitations, the integration of metal nanoparticles has emerged as a promising strategy for the efficient delivery of herbal extracts, affording heightened bioavailability and precise targeting of the gut microbiota. In this paper, we present current breakthrough in metal infused nanoparticle-based assessment of herbal extracts as efficient prebiotics, with an emphasis on formulation, characterization, and biological activity. The integration of metal nanoparticles into polyphenol-rich herbal extracts represents a cutting-edge approach to enhancing the prebiotic properties of these compounds. Metal-infused polyphenol-enriched phyto-fabricated nanoparticles hold great promise for improving gut health and modulating the gut microbiota, offering innovative solutions to address the limitations associated with traditional polyphenol delivery. Furthermore, extracts of polyphenol-rich medicinal herbs such as rosehip flower, hibiscus flower, mango bark, bamboo stem, green tea, pomegranate, and dhataki flower have been investigated to assess the viability of employing these phyto generated metal infused nanoparticles as a potential prebiotic

show abstract

Preparation and Evaluation of the Antibacterial Effect of Chitosan Nanoparticles Containing Ginger Extract Tailored by Central Composite Design

Abstract: The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Cited by 15 publications

References 35 publications

Hyaluronic Acid‐Coated Chitosan/Gelatin Nanoparticles as a New Strategy for Topical Delivery of Metformin in Melanoma

Hyaluronic Acid‐Coated Chitosan/Gelatin Nanoparticles as a New Strategy for Topical Delivery of Metformin in Melanoma

Preparation, characterisation and comparative toxicity of nanopermethrin against Anophelesstephensi and Culexpipiens

Metal-Infused Polyphenol-enriched Phyto-fabricated Nanoparticles: an In-depth Review of their Potent Prebiotic Properties

Contact Info

Product

Resources

About