Preparation and functional evaluation of oligopeptide-enriched hydrolysate from shrimp (Acetes chinensis) treated with crude protease from Bacillus sp. SM98011

He, Hui; Chen, Xiu-Lan; Sun, Caiyun; Zhang, Yu-Zhong; Gao, Pin

doi:10.1016/j.biortech.2005.03.016

Cited by 137 publications

(73 citation statements)

References 17 publications

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“…It has been reported that chitin, chitosan and peptide have antioxidative (Pinero Estrada et al 2001;Lin and Chou 2004;Xing et al 2005;He et al 2006;Wang et al 2009) and anticarcinogenic (Liang et al 2007;Wang et al 2008a) properties. To increase the utilization of these chitin/protein-containing shellfish wastes, we incubated Serratia sp.…”

Section: Degradation Of Shellfish Wastes and Production Of Antioxidantsmentioning

confidence: 98%

Conversion and degradation of shellfish wastes by Serratia sp. TKU016 fermentation for the production of enzymes and bioactive materials

2009

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A chitosanase and a protease were purified from the culture supernatant of Serratia sp. TKU016 with shrimp shell as the sole carbon/nitrogen source. The molecular masses of the chitosanase and protease determined by SDS-PAGE were approximately 65 and 53 kDa, respectively. The chitosanase was inhibited completely by Mn2+, but the protease was enhanced by all of tested divalent metals. The optimum pH, optimum temperature, pH stability, and thermal stability of the chitosanase and protease were (pH 7, 50 degrees C, pH 6-7, <50 degrees C) and (pH 8-10, 40 degrees C, pH 5-10, <50 degrees C), respectively. SDS (2 mM) had stimulatory effect on TKU016 protease activity. The result demonstrates that TKU016 protease is SDS-resistant protease and probably has a rigid structure. Besides, TKU016 culture supernatant (2% SPP) incubated for 2 days has the highest antioxidant activity, the DPPH scavenging ability was about 76%. With this method, we have shown that shrimp shell wastes can be utilized and it's effective in the production of enzymes, antioxidants, peptide and reducing sugar, facilitating its potential use in biological applications and functional foods.

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Section: Degradation Of Shellfish Wastes and Production Of Antioxidantsmentioning

confidence: 98%

Conversion and degradation of shellfish wastes by Serratia sp. TKU016 fermentation for the production of enzymes and bioactive materials

2009

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“…Different initiative for recovery of protein and amino acids (Mandeville et al 1992), colourants (Chen and Meyers 1982), flavourants (Pan 1990) and chitin, chitosan (Coward-kelly et al 2006) from shrimp head and shell has been undertaken of which enzymatic hydrolysis approach was effective (Ruttanapornvareesakul et al 2006). Very few research has been undertaken regarding antioxidative effect of protein hydrolysate or peptides from fish and shellfish, as well as their byproducts (He et al 2006). However, little information regarding antioxidants of a protein concentrate from shrimp waste and its application has been reported.…”

Section: Introductionmentioning

confidence: 99%

Antioxidative activity of protein hydrolysate produced by alcalase hydrolysis from shrimp waste (Penaeus monodon and Penaeus indicus)

Dey

Dora

2011

J Food Sci Technol

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Protein hydrolysates prepared by hydrolysis of shrimp waste (Penaeus monodon and Penaeus indicus) for 90 min. using Alcalase enzyme following pH-stat method. Antioxidative activities of SWPH were assessed determining FRAP, ABTS and DPPH radical scavenging activities, which increased linearly with increasing concentration of protein hydrolysate upto 5 mg/ml maintaining good correlation. SWPH showed high stability over wide ranges of pH (2-11) and temperature (up to 100°C for 150 min), in which the activity of more than 80% was retained. Protein hydrolysate solution with a concentration of 5 mg/ml significantly lowered TBA values of Croaker fish fillet and maintained yellowishness of skin colour compared to untreated control sample during 10 days of refrigerated storage at 4°C. SWPH also restricted the increase of PV and FFA values in Croaker fish fillet within acceptable limit.

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“…This means that Lactobacillus and its fermentation products are acceptable to humans. It has also been well documented that short peptides produced by enzymatic hydrolysis and fermentation from different sources of protein have immunomodulatory effects, and antimicrobial and antioxidant activity (Gibbs et al, 2004;He et al, 2006;Kristinsson and Rasco, 2000). The culture supernatants of TKU010 and TKU012 were prepared under the optimal culture conditions of extracellular protease production (Wang et al, 2008a, c) and the culture supernatants of L. paracasei subsp.…”

Section: Enhancement Of Growthmentioning

confidence: 99%

“…Besides, it has been reported that peptides have antioxidative (He et al, 2006;Lin and Chou, 2004;Pinero Estrada et al, 2001;Xing et al, 2005) The supernatants of different culture times were prepared by using squid pen powder (1%) as a sole carbon/nitrogen source and treated in E. coli BCRC13086 growth. , control; , the first day culture supernatant; , the second day culture supernatant; , the third day culture supernatant; , the fourth day culture supernatant.…”

Section: Antioxidant Property Of the Culture Supernatantmentioning

confidence: 99%

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Conversion of squid pen by a novel strain Lactobacillus paracasei subsp. paracasei TKU010, and its application in antimicrobial and antioxidants activity

Liang

Huang

et al. 2010

J. Gen. Appl. Microbiol.

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TKU010 was isolated from infant vomited milk and identifi ed as Lactobacillus paracasei subsp. paracasei. TKU010 had desirable properties concerning its ability to withstand adverse conditions in the gastrointestinal tract. The hydrolysate of casein enhanced the growth of TKU010 most obviously (17.20 18.25 OD 660 ), followed by the hydrolysate of SPP (16.00 15.06 OD 660 ). Incubating with SPP, both the culture supernatant of TKU010 on the fi rst day and the fourth day showed inhibitory activities on E. coli BCRC13086, F. oxysporum BCRC32121 and A. fumigatus BCRC30099. TKU010 culture supernatant (1% SPP) incubated for 3 days has high antioxidant activity; the DPPH scavenging ability was 75% per ml. Thus, TKU010 could be preferably used as a starter to produce fermented milk with possibly interesting organoleptic properties. Besides, we have shown that squid pen wastes can be utilized to generate a high value-added product, and have revealed its hidden potential in the production of biocontrol agents and functional foods.

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Preparation and functional evaluation of oligopeptide-enriched hydrolysate from shrimp (Acetes chinensis) treated with crude protease from Bacillus sp. SM98011

Cited by 137 publications

References 17 publications

Conversion and degradation of shellfish wastes by Serratia sp. TKU016 fermentation for the production of enzymes and bioactive materials

Conversion and degradation of shellfish wastes by Serratia sp. TKU016 fermentation for the production of enzymes and bioactive materials

Antioxidative activity of protein hydrolysate produced by alcalase hydrolysis from shrimp waste (Penaeus monodon and Penaeus indicus)

Conversion of squid pen by a novel strain Lactobacillus paracasei subsp. paracasei TKU010, and its application in antimicrobial and antioxidants activity

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