Preparation and<i>in vitro</i>,<i>in vivo</i>evaluations of norfloxacin-loaded solid lipid nanopartices for oral delivery

Zhao, Dong; Xie, Shuyu; Zhu, Luyan; Wang, Yan; Wang, Xiaofang; Zhou, Wenzhong

doi:10.3109/10717544.2011.577109

Cited by 31 publications

(28 citation statements)

References 65 publications

(62 reference statements)

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“…The rational design of MgNPs comprised the use of PVA as an emulsifier and stabilizing agent. PVA confers remarkably low cytotoxicity and a negative zeta potential to nanoparticles (DeMerlis and Schoneker, 2003;Roy et al 2010;Dong et al 2011). Charges on the nanoparticle surface produce repulsive electrostatic forces that prevent aggregation and help to stabilize the suspension (Min et al 2008).…”

Section: Magnetite-loaded Phbv Nanoparticles (Mgnps)mentioning

confidence: 95%

Superparamagnetic Poly (3-hydroxybutyrate-co-3 hydroxyvalerate) (PHBV) nanoparticles for biomedical applications.

Vilos¹,

Gutiérrez²,

Escobar³

et al. 2013

Electron. J. Biotechnol.

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Background:The progress in material science and the recent advances in biodegradable/biocompatible polymers and magnetic iron oxide nanoparticles have led to develop innovative diagnostic and therapeutic strategies for diseases based on multifunctional nanoparticles, which include contrast medium for magnetic resonance imaging, agent for hyperthermia and nanocarriers for targeted drug delivery. The aim of this work is to synthesize and characterize superparamagnetic iron oxide (magnetite), and to encapsulate them into poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanoparticles for biomedical applications. Results: The magnetite nanoparticles were confirmed by X-ray diffraction and exhibited a size of 22.3 ± 8.8 nm measured by transmission electron microscopy (TEM). Polymeric PHBV nanoparticles loaded with magnetite (MgNPs) were analyzed using dynamic light scattering and showed a size of 258.6 ± 35.7 nm and a negative zeta potential (-10.8 ± 3.5 mV). The TEM examination of MgNPs exhibited a spherical core-shell structure and the magnetic measurements showed in both, non-encapsulated magnetite and MgNPs, a superparamagnetic performance. Finally, the in vitro studies about the magnetic retention of MgNPs in a segment of small intestine of rats showed an active accumulation in the region of the magnetic field. Conclusions: The results obtained make the MgNPs suitable as potential magnetic resonance imaging contrast agents, also promoting hyperthermia and even as potential nanocarriers for sitespecific transport and delivery of drugs.

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Section: Magnetite-loaded Phbv Nanoparticles (Mgnps)mentioning

confidence: 95%

Superparamagnetic Poly (3-hydroxybutyrate-co-3 hydroxyvalerate) (PHBV) nanoparticles for biomedical applications.

Vilos¹,

Gutiérrez²,

Escobar³

et al. 2013

Electron. J. Biotechnol.

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“…Studies of SLNs loaded with lipophilic drugs have yielded promising results. For example, the antibacterial responses of norfloxacin were enhanced when the drug was loaded into SLNs [4]. Moreover, Bose and Michniak observed that SLN encapsulation increased the gastrointestinal absorption of quercetin, as indicated by an increase in the drug bioavailability of 571.4% [5].…”

Section: Introductionmentioning

confidence: 99%

Physicochemical characterization by AFM, FT-IR and DSC and biological assays of a promising antileishmania delivery system loaded with a natural Brazilian product

Marquele-Oliveira

Torres

Barud

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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The control and treatment of Leishmaniasis, a neglected and infectious disease affecting approximately 12 million people worldwide, are challenging. Leishmania parasites multiply intracellularly within macrophages located in deep skin and in visceral tissues, and the currently employed treatments for this disease are subject to significant drawbacks, such as resistance and toxicity. Thus, the search for new Leishmaniasis treatments is compulsory, and Ocotea duckei Vattimo, a plant-derived product from the biodiverse Brazilian flora, may be a promising new treatment for this disease. In this regard, the aim of this work was to develop and characterize a delivery system based on solid lipid nanoparticles (SLN) that contain the liposoluble lignan fraction (LF) of Ocotea duckei Vattimo, which targets the Leishmania phagolysosome of infected macrophages. LF-loaded SLNs were obtained via the hot microemulsion method, and their physical and chemical properties were comprehensively assessed using PCS, AFM, SEM, FT-IR, DSC, HPLC, kinetic drug release studies, and biological assays. The size of the developed delivery system was 218.85±14.2 nm, its zeta potential was -30 mV and its entrapment efficiency (EE%) was high (the EEs% of YAN [yangambin] and EPI-YAN [epi-yangambin] markers were 94.21±0.40% and 94.20±0.00%, respectively). Microscopy, FT-IR and DSC assays confirmed that the delivery system was nanosized and indicated a core-shell encapsulation model, which corroborated the measured kinetics of drug release. The total in vitro release rates of YAN and EPI-YAN in buffer (with sink conditions attained) were 29.6±8.3% and 34.3±8.9%, respectively, via diffusion through the cellulose acetate membrane of the SLN over a period of 4 h. After 24 h, the release rates of both markers reached approximately 45%, suggesting a sustained pattern of release. Mathematical modeling indicated that both markers, YAN and EPI-YAN, followed matrix diffusion-based release kinetics (Higuchi's model) with an estimated diffusion coefficient (D) of 1.3.10(-6) cm(2)/s. The LF-loaded SLNs were non-toxic to murine macrophages (20-80 μg mL(-1) range) and exerted a prominent anti-leishmanial effect (20 μg mL(-1)). These data suggest this new and well-characterized lipid nanoparticle delivery system safely and effectively kills Leishmania and warrants further clinical investigation.

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“…26 The lipid phase was prepared by melting stearic acid, lecithin as well as PE at 10°C above the lipid melting point. Meanwhile, the dissolving of tween 80 in deionized water and then heating to the same temperature of the lipid phase was carried out for the preparation of the aqueous phase.…”

Section: Preparation Of Pe-slnsmentioning

confidence: 99%

Pomegranate extract-loaded solid lipid nanoparticles: design, optimization, and in vitro cytotoxicity study

Badawi¹,

Teaima²,

El-Say³

et al. 2018

IJN

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Background Pomegranate extract (PE) is a natural product with potent antioxidant and anticancer activity because of its polyphenols content. The main purpose of this study was to maximize the PE chemotherapeutic efficacy by loading it in an optimized solid lipid nanoparticles (SLNs) formula. Materials and methods The influence of independent variables, which were lipid concentration (X 1 ), surfactant concentration (X 2 ) and cosurfactant concentration (X 3 ), on dependent ones, which were particle size (Y 1 ), polydispersity index (Y 2 ), zeta potential (Y 3 ), entrapment efficiency (Y 4 ) and cumulative % drug release (Y 5 ), were studied and optimized using the Box–Behnken design. Fifteen formulations of PE-SLNs were prepared using hot homogenization followed by ultra-sonication technique. Response surface plots, Pareto charts and mathematical equations were produced to study the impact of independent variables on the dependent quality parameters. The anti-proliferative activity of the optimized formula was then evaluated in three different cancer cell lines, namely, MCF-7, PC-3 and HepG-2, in addition to one normal cell line, HFB-4. Results The results demonstrated that the particle sizes ranged from 407.5 to 651.9 nm and the entrapment efficiencies ranged from 56.02 to 65.23%. Interestingly, the 50% inhibitory concentration of the optimized formula had more than a 40-fold improved effect on the cell growth inhibition in comparison with its free counterpart. Furthermore, it was more selective against cancer cells than normal cells particularly in MCF-7 breast cancer cells. Conclusion These data proved that nanoencapsulation of PE enhanced its anticancer efficacy. Therefore, our results suggested that a PE-loaded SLNs optimized-formula could be a promising chemo therapeutic agent.

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Preparation andin vitro,in vivoevaluations of norfloxacin-loaded solid lipid nanopartices for oral delivery

Abstract: ) Preparation and invitro, invivo evaluations of norfloxacin-loaded solid lipid nanopartices for oral delivery, Drug Delivery, 18:6, 441-450,

Cited by 31 publications

References 65 publications

Superparamagnetic Poly (3-hydroxybutyrate-co-3 hydroxyvalerate) (PHBV) nanoparticles for biomedical applications.

Superparamagnetic Poly (3-hydroxybutyrate-co-3 hydroxyvalerate) (PHBV) nanoparticles for biomedical applications.

Physicochemical characterization by AFM, FT-IR and DSC and biological assays of a promising antileishmania delivery system loaded with a natural Brazilian product

Pomegranate extract-loaded solid lipid nanoparticles: design, optimization, and in vitro cytotoxicity study

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