Preparation and Modification of Collagen/Sodium Alginate-Based Biomedical Materials and Their Characteristics

Abstract

(1) Background: Collagen and sodium alginate are commonly used in the field of biomedical materials due to their excellent biocompatibility. This study focuses on the preparation, modification, and characterization of collagen/sodium alginate (C/SA)-based biomedical materials. (2) Methods: The characteristics, including surface chemistry, mechanical properties, hygroscopicity, and porosity, were analyzed. The hemostatic activity in vitro was measured using a blood clotting assay and dynamic blood clotting assay. (3) Results: The results from microstructure and porosity measurement revealed that all of the sponges exhibited a porosity of more than 95 percent. The sponge cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) showed better tensile strength and lower elongation at break. The sponges cross-linked with EDC/NHS and oxidized sodium alginate (OSA) exhibited the highest hygroscopicity in comparison with the uncross-linked sponge. (4) Conclusions: Our study demonstrated that the C/SA-based material we prepared exhibited a high level of porosity, enabling efficient absorption of tissue exudate and blood. Additionally, the materials revealed excellent hemocompatibility, making them suitable for use as a hemostatic dressing in the field of biomedical materials.

Comprehensive Assessment of Collagen/Sodium Alginate-Based Sponges as Hemostatic Dressings

“…In our previous research, dressings treated with EDC/NHS and OSA demonstrated good solution stability and hygroscopicity, with values of 25.93 ± 0.56 and 25.12 ± 0.43, respectively [50]. The crosslinking of EDC/NHS and OSA resulted in a shift in the amide SA peak from 3315 cm −1 to approximately 3282 cm −1 [50]. The stretching peaks of the O-H and N-H bonds were notably enhanced [50].…”

“…Additionally, no carbodiimide residue was detected in the EDC/NHS cross-linked C/SA dressing. In our previous research, dressings treated with EDC/NHS and OSA demonstrated good solution stability and hygroscopicity, with values of 25.93 ± 0.56 and 25.12 ± 0.43, respectively [50]. The crosslinking of EDC/NHS and OSA resulted in a shift in the amide SA peak from 3315 cm −1 to approximately 3282 cm −1 [50].…”

“…The stretching peaks of the O-H and N-H bonds were notably enhanced [50]. OSA displayed a characteristic vibrational peak of the aldehyde group at 1615 cm −1 , and the C/OSA dressing exhibited an absorption peak of the Schiff base structure at 1653 cm −1 [50]. The successful introduction of the carboxyl group was evidenced by the disappearance of the absorption peak at 1417 cm −1 and the appearance of two new absorption peaks at 1445 cm −1 and 1409 cm −1 [50].…”

“…The crosslinking of EDC/NHS and OSA resulted in a shift in the amide SA peak from 3315 cm −1 to approximately 3282 cm −1 [50]. The stretching peaks of the O-H and N-H bonds were notably enhanced [50]. OSA displayed a characteristic vibrational peak of the aldehyde group at 1615 cm −1 , and the C/OSA dressing exhibited an absorption peak of the Schiff base structure at 1653 cm −1 [50].…”

“…OSA displayed a characteristic vibrational peak of the aldehyde group at 1615 cm −1 , and the C/OSA dressing exhibited an absorption peak of the Schiff base structure at 1653 cm −1 [50]. The successful introduction of the carboxyl group was evidenced by the disappearance of the absorption peak at 1417 cm −1 and the appearance of two new absorption peaks at 1445 cm −1 and 1409 cm −1 [50]. In terms of SEM analysis, the cross-linked sponges and unmodified sponges showed structural similarity and were characterized by their porous nature, which facilitated cell growth [50].…”