Preparation and properties of a drug release membrane of mitomycin C with N-succinyl-hydroxyethyl chitosan

Li, Minyu; Han, Baoqin; Liu, Wanshun

doi:10.1007/s10856-011-4455-8

Cited by 9 publications

(4 citation statements)

References 34 publications

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“…On the other hand, the chitosan cross-linked with GA membranes and the sample neutralized and cross-linked with GA shows a swelling degree of 1000 % after 72 h in PBS solution (equation 2) which is quite high when compared to the swelling index of ~100 % found by Sangsanoh [6] or 600 % found by Li et al [28]. Knault et al suggests that the swelling of chitosan films is mainly influenced by ionic interactions between polymer chains, and depends on the cross-linking density achieved during the formation of the biopolymer network.…”

Section: After Neutralization 219±48mentioning

confidence: 83%

Physical-chemical properties of cross-linked chitosan electrospun fiber mats

et al. 2012

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Chitosan fiber mats were successfully processed by electrospinning. The as-spun fiber mats were neutralized with ethanol and cross-linked with glutaraldehyde. A decrease of the fiber average diameter from 243 ± 43 nm down to 215.53 nm was observed for the neutralized and cross-linking chitosan membrane. It was found that the processing conditions do not alter the initial deacetylation degree of the polymer. Polymer crystallinity index showed a decrease from 61 % for the Protasan material down to 17 % for the cross-linking fiber mats. A swelling index up to 1000 % was observed for the cross-linked samples. Preliminary MC-3T3-E1cell culture results showed good cell adhesion and proliferation in the cross-linked chitosan fiber mats.

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Section: After Neutralization 219±48mentioning

confidence: 83%

Physical-chemical properties of cross-linked chitosan electrospun fiber mats

et al. 2012

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“…[ 23 25 ] It is valuable for the drug carrier to readily prepare its conjugates with various drugs to avoid vexatious complications. [ 26 ] Theoretically, a chemical combination could happen between PTX and SHEC in the presence of an appropriate catalyst. Then, the membrane of PTX-SHEC may be prepared using cross-linked modification.…”

Section: Discussionmentioning

confidence: 99%

Effects of Paclitaxel-conjugated N-Succinyl-Hydroxyethyl Chitosan Film for Proliferative Cholangitis in Rabbit Biliary Stricture Model

Wang

Zou

Zhang

2018

Chinese Medical Journal

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Background:Paclitaxel (PTX) could inhibit the growth of fibroblasts, which occurs in proliferative cholangitis and leads to biliary stricture. However, its use has been limited due to poor bioavailability and local administration for short time. This study designed and synthesized a new PTX-conjugated chitosan film (N-succinyl-hydroxyethyl chitosan containing PTX [PTX-SHEC]) and evaluated its safety and efficiency using in vivo and in vitro experiments.Methods:The SHEC conjugated with PTX was confirmed by nuclear magnetic resonance (NMR) and Fourier-transform infrared spectroscopy (FT-IR) measurements. Drug releases in vitro and in vivo were determined using high-performance liquid chromatography. Cell viability in vitro was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. Rabbit biliary stricture model was constructed. All rabbits randomly divided into five groups (n = 8 in each group): the sham-operated rabbits were used as control (Group A), Groups B received laparotomies and suture, Group C received laparotomies and covered SHEC suture without the PTX coating, Group D received laparotomies and covered PTX-SHEC suture, and Group E received laparotomies and 1000 μmol/L PTX administration. Liver function tests and residual dosage of PTX from each group were measured by enzyme-linked immunosorbent assay. Histological data and α-smooth muscle actin (SMA) immunohistochemical staining of common bile duct were examined.Results:NMR and FT-IR indicated that PTX was successfully introduced, based on the appearance of signals at 7.41–7.99 ppm, 1.50 ppm, and 1.03 ppm, due to the presence of aromatic protons, methylene protons, and methyl protons of PTX, respectively. No bile leak was observed. The PTX-conjugated film could slowly release PTX for 4 weeks (8.89 ± 0.03 μg at day 30). The in vitro cell viability test revealed significantly different levels of toxicity between films with and without PTX (111.7 ± 4.0% vs. 68.1 ± 6.0%, P < 0.001), whereas no statistically significant difference was observed among the three sets of PTX-contained films (67.7 ± 5.4%, 67.2 ± 3.4%, and 59.1 ± 6.0%, P > 0.05). Histological examinations revealed that after 28 days of implantment, Groups D and E (but not Group C) had less granulation tissue and glandular hyperplasia in the site of biliary duct injury than Group B. The pattern was more obvious in Group D than Group E. Less α-SMA-positive cells were found in tissue from Groups D and E. Comparing with Group E, the liver function was improved significantly in Group D, including total bilirubin (2.69 ± 1.03 μmol/L vs. 0.81 ± 0.54 μmol/L, P = 0.014), alanine aminotransferase (87.13 ± 17.51 U/L vs. 42.12 ± 15.76 U/L, P = 0.012), and alkaline phosphatase (60.61 ± 12.31 U/L vs. 40.59 ± 8.78 U/L, P < 0.001).Conclusions:PTX-SHEC film effectively inhibites the myofibroblast proliferation and extracellular matrix over-deposition during the healing process of biliary reconstruction. This original film might offer a new way for reducing the occurrence of the benign biliary ...

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“…The absorption bands were characterized and compared with other MMC FTIR analyses. 30,34,40 Broadband at 3,448 cm -1 was observed, which refers to the characteristic absorption peaks of the combination of OH, NH, and NH 2 groups. Peaks at 2,860 and 2,926 cm -1 correspond to the CH aromatic band, and the absorption bands at 1,000 to 1,250 cm -1 were assigned to C-O and Si-O.…”

Section: Mitomycin Loading Into Psi Carriersmentioning

confidence: 98%

“…Several methods have been investigated for the delivery of MMC in nano or micro size. Common methods for MMC delivery consist of encapsulation of the drug in nano-or micro-particles, using biodegradable polymers including albumin, 27 dextran, 28 estradiol, 29 N-succinyl-chitosan, 30 hydrogels, 31 polybutylcyanoacrylate, 32 poly-epsilon-caprolactone, 33 oxidized-dextran microspheres, 28 and polylactic acids (PLA) nanoparticles. 34 However, to our knowledge, MMC has never been loaded into pSi.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Mitomycin C-Porous Silicon in Human Prostate Carcinoma Cells

Ali¹,

Haidary²,

Omer³

et al. 2020

ijddt

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Porous silicon (pSi) microparticles with pore diameter 15 to 20 nm were fabricated by electrochemical etching of single-crystalline silicon (Si) wafers (n-type) to be used as delivery systems for the anticancer drug mitomycin C (MMC). The in vitro toxicity of the mitomycin-loaded pSi carrier was investigated on human prostate carcinoma (DU145) cells. The cells showed a decrease in viability of ~80% over a 6 hours period, when using mitomycin. Meanwhile, a ~55% decrease in cell viability was observed, when using the pSi carrier to deliver the drug. The drug-loaded carrier showed a sustained release throughout 24 hours, with an 80% decrease in cell viability after 16 hours. This observed controlled release of mitomycin from the pSi carrier suggests a superior therapeutic effect than the direct administration of mitomycin, as it potentially minimizes the drug side effects. Results showed that the strong cytotoxic effect towards the prostate cancer cells was due to the drug and not the carrier since the mitomycin-loaded pSi carrier affected cell viability, but the pSi carrier showed no toxicity. Furthermore, it was observed that with a higher amount of drug-loaded carriers, the toxicity effect was higher, thus, allowing further control of the therapeutic effect of the carrier.

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Preparation and properties of a drug release membrane of mitomycin C with N-succinyl-hydroxyethyl chitosan

Cited by 9 publications

References 34 publications

Physical-chemical properties of cross-linked chitosan electrospun fiber mats

Physical-chemical properties of cross-linked chitosan electrospun fiber mats

Effects of Paclitaxel-conjugated N-Succinyl-Hydroxyethyl Chitosan Film for Proliferative Cholangitis in Rabbit Biliary Stricture Model

Cytotoxicity of Mitomycin C-Porous Silicon in Human Prostate Carcinoma Cells

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