Preparation and properties of arsonolipid containing liposomes

Fatouros, Dimitrios G.; Gortzi, Olga; Klepetsanis, Pavlos; Antimisiaris, Sophia G.; Stuart, Marc C. A.; Brisson, Alain; Ioannou, Panagiotis

doi:10.1016/s0009-3084(00)00221-8

Cited by 48 publications

(34 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Arsonolipid (Ars)-containing liposomes were prepared as described previously [1,2,6,7]. In brief, lipids (after removing the organic solvents with a nitrogen stream) were mixed with 5 mM phosphate buffer (pH 7.4) and 20 mM NaCl and magnetically stirred vigorously on a hot plate for 4 h at 70 -808C.…”

Section: Preparation Of Arsonoliposomesmentioning

confidence: 99%

“…1), referred to as , arsonoliposomes' have been prepared and characterized in our laboratories previously [1]. As summarized before [2], promising results were obtained with some of the arsonoliposome types prepared, for which a differential toxicity towards cancer and normal cells was demonstrated [3,4] as well as in vitro antiparasitic activity [5].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Does the lipid membrane composition of arsonoliposomes affect their anticancer activity? A cell culture study

Zagana

Haikou

Giannopoulou

et al. 2009

Molecular Nutrition Food Res

View full text Add to dashboard Cite

Sonicated arsonoliposomes were prepared using arsonolipid with palmitic acid acyl chain (C16), mixed with phosphatidylcholine (PC)-based or 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)-based, and cholesterol (Chol) with C16/DSPC/Chol 8:12:10 molar ratio. PEG-lipid (1,2-distearoyl-sn-glycero-3-phosphoethanolamine conjugated to polyethylenoglycol 2000) containing vesicles (PEGylated-arsonoliposomes; PC-based and DSPC-based) were also prepared. The cytotoxicity of these arsonoliposomes towards different cancer cells (human promyelocytic leukaemia NB4, Prostatic cancer PC3, human breast adenocarcinoma MDA-MB-468, human T-lymphocyte (MT-4) and also towards human umbilical vein endothelial cells (HUVECs) was evaluated by calculating the arsonoliposome-induced growth inhibition of the cells by the MTT assay. IC-50 values were interpolated from cell number/arsonoliposome concentration curves. The results reveal that all types of arsonoliposomes evaluated significantly inhibit the growth of most of the cancer cells studied (PC3, NB4, MT4) with the exception of the MDA-MB-468 breast cancer cells which were minimally affected by arsonoliposomes; in some cases even less than HUVEC. Nevertheless, for the same cell type the differences between the different types of arsonoliposomes were significant but not proportional to their stability, indicating that the formation of arsonoliposomes with very stable membranes is not a problem for their anticancer activity. Thereby it is concluded that arsonoliposome composition should be adjusted in accordance to their in vivo kinetics and the desired, for each specific application, biodistribution of As and/or encapsulated drug.

show abstract

Section: Preparation Of Arsonoliposomesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Does the lipid membrane composition of arsonoliposomes affect their anticancer activity? A cell culture study

Zagana

Haikou

Giannopoulou

et al. 2009

Molecular Nutrition Food Res

View full text Add to dashboard Cite

show abstract

“…The difference in the transport rate constant calculated in Table 1 for the two cases is due to the large difference in C A,0 , in the two experiments. The previously demonstrated effect of arsonolipid fatty acyl chain length on physicochemical properties of the lipids (Serves et al, 1993), as well as on the morphology, size and stability of the vesicles they form (Fatouros et al, 2001), prompted us to study the transport of Ca 2 + by the C 12 and C 16 arsonolipids, as well. From Fig.…”

Section: Resultsmentioning

confidence: 99%

“…We have recently prepared unilamellar and multilamellar vesicle containing arsonolipids that were demonstrated to have negative zeta potential values, linearly correlated with the arsonolipid content of their membrane (Fatouros et al, 2001). In addition, in very recent studies (Gortzi et al, unpublished results) the presence of Ca 2 + has been demonstrated to affect the vesicle zeta potential substantially more in vesicles that have higher arsonolipid contents.…”

Section: Resultsmentioning

confidence: 99%

“…In a recent study (Fatouros et al, 2001), it was found that arsonolipids form tubules (C 12 ) or disk-shaped liposomes (C 14 , C 16 , C 18 ), when dispersed alone in aqueous media -under appropriate conditions -while admixed with phosphatidylcholine and/or cholesterol, form more conventional liposomes (arsonoliposomes), in respect of vesicle shape. These latter liposomes can be used either empty (as an As transferring system) or after being loaded with drugs, as drug delivery systems.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Capability of arsonolipids to transport divalent cations: a Pressman cell study

Gortzi

Klepetsanis

Antimisiaris

et al. 2001

Chemistry and Physics of Lipids

View full text Add to dashboard Cite

The ability of the newly synthesized arsonolipids (2,3-diacyloxypropylarsonic acids) to transport cations was studied using the Pressman cell. Experimental results demonstrate that arsonolipids are much more efficient carriers of Ca 2 + and Mg 2 + than natural phosphatidic acid in the Pressman cell experiments. The ability of arsonolipids to transfer Ca 2 + is affected by the lipid side chain length in the order: C 12 C 14 $ C 16 . Ca 2 + is transferred faster than Mg 2 + , suggesting that the latter is more tightly bound to the arsonolipids. The transfer kinetic curves are parabolic for C 12 , while initially linear with a tendency to reach a steady state for C 14 and C 16 , when the pH in the donor compartment was 8.3. The transport kinetics for both ions studied were best fitted by an equation derived from saturation kinetics that apply in reversible chemical reactions. The ion transfer rates increased as the pH in the donor compartment decreased.

show abstract

Determination of lipid‐soluble arsenic species in seaweed‐eating sheep from Orkney

Devalla

Feldmann

2003

Applied Organom Chemis

View full text Add to dashboard Cite

This work is part of an ongoing research study towards an understanding of the complete metabolism of arsenosugars in mammalian organisms when ingesting seaweed, using the North Ronaldsay (NR) sheep as a model organism. We focus on the analysis of only those arsenic species bound to the lipids of the feed (Laminaria digitata), faeces and the tissues of the NR sheep using a novel enzymatic hydrolytic method that is simple and reliable. This rare breed of sheep, found in the remote Orkney Islands in the north of Scotland, live the entire year on the beaches and eat seaweed that is washed ashore (up to 3 kg daily).Previous studies on arsenic fractionation in muscle, kidney and liver tissues revealed that most of the arsenic is concentrated in the fat fractions of these tissues (muscle fat: 61%; liver fat: 66%; kidney fat: 25%) rather than in the non-lipid fractions. Hence, this study was undertaken in order to determine the arsenic species bound to lipids in the muscle, kidney and faeces of NR sheep and to compare these with the arsenic species bound to the lipids of the L. digitata consumed.The enzymatic hydrolytic procedure has been successfully employed for the first time to cleave the arsenic species cleanly from the rest of the lipid structure. This makes the arsenic species water soluble and enables their direct determination by high-performance liquid chromatography coupled with inductively coupled plasma mass spectrometry. Dimethylarsinic acid (DMA(V)) and monomethylarsonic acid (MA(V)) were found to be the major hydrolysed arsenic species bound to the kidney and muscle lipids, whereas arsenosugar-1 was found to be the major hydrolysed arsenic species in L. digitata lipids. On the other hand, DMA(V) was found to be the major arsenical obtained after the enzymatic hydrolysis of the faeces lipids. These results seem to suggest that both direct absorption and biotransformation of the absorbed organoarsenicals are the likely reasons for their occurrence and accumulation in the NR sheep tissues.

show abstract

Preparation and properties of arsonolipid containing liposomes

Cited by 48 publications

References 26 publications

Does the lipid membrane composition of arsonoliposomes affect their anticancer activity? A cell culture study

Does the lipid membrane composition of arsonoliposomes affect their anticancer activity? A cell culture study

Capability of arsonolipids to transport divalent cations: a Pressman cell study

Determination of lipid‐soluble arsenic species in seaweed‐eating sheep from Orkney

Contact Info

Product

Resources

About