Preparation and properties of BSA-loaded microspheres based on multi-(amino acid) copolymer for protein delivery

Chen, Xingtao; Lv, Guoyu; Zhang, Jue; Tang, Songchao; Yan, Yonggang; Wu, Zhaoying; Su, Jiacan; Wei, Jie

doi:10.2147/ijn.s57048

Cited by 18 publications

(11 citation statements)

References 29 publications

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“…In this review, silk fibroin [ 11 ], keratin [ 12 ], collagen [ 13 ], gelatin [ 14 ], elastin [ 15 ], corn zein [ 16 ], and soy protein [ 17 ] will be given particular attention due to their popularity in biomaterials research ( Figure 1 ). However, additional protein polymers such as casein [ 18 ], fibrinogen [ 18 ], hemoglobin [ 19 ], bovine serum albumin [ 20 ], gluten [ 20 ] have also been used to create nanoparticles.…”

Section: Categories Of Protein Materialsmentioning

confidence: 99%

Protein Polymer-Based Nanoparticles: Fabrication and Medical Applications

DeFrates

Markiewicz

Gallo

et al. 2018

IJMS

182

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Nanoparticles are particles that range in size from about 1–1000 nanometers in diameter, about one thousand times smaller than the average cell in a human body. Their small size, flexible fabrication, and high surface-area-to-volume ratio make them ideal systems for drug delivery. Nanoparticles can be made from a variety of materials including metals, polysaccharides, and proteins. Biological protein-based nanoparticles such as silk, keratin, collagen, elastin, corn zein, and soy protein-based nanoparticles are advantageous in having biodegradability, bioavailability, and relatively low cost. Many protein nanoparticles are easy to process and can be modified to achieve desired specifications such as size, morphology, and weight. Protein nanoparticles are used in a variety of settings and are replacing many materials that are not biocompatible and have a negative impact on the environment. Here we attempt to review the literature pertaining to protein-based nanoparticles with a focus on their application in drug delivery and biomedical fields. Additional detail on governing nanoparticle parameters, specific protein nanoparticle applications, and fabrication methods are also provided.

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Section: Categories Of Protein Materialsmentioning

confidence: 99%

Protein Polymer-Based Nanoparticles: Fabrication and Medical Applications

DeFrates

Markiewicz

Gallo

et al. 2018

IJMS

182

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show abstract

“…In addition, in the gastrointestinal tract a decrease in the pH value of the adjacent microenvironment may induce side effects such as infl ammation. [33][34][35] Hence, it is essential to explore new biomaterials with versatile encapsulation strategies to meet the ever increasing demand for encapsulating biomaterials.…”

Section: Macromolecular Encapsulation and Characterization Of Naturalmentioning

confidence: 99%

Lycopodium Spores: A Naturally Manufactured, Superrobust Biomaterial for Drug Delivery

Mundargi

Potroz

Park

et al. 2015

Adv Funct Materials

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Herein, the exploration of natural plant‐based “spores” for the encapsulation of macromolecules as a drug delivery platform is reported. Benefits of encapsulation with natural “spores” include highly uniform size distribution and materials encapsulation by relatively economical and simple versatile methods. The natural spores possess unique micromeritic properties and an inner cavity for significant macromolecule loading with retention of therapeutic spore constituents. In addition, these natural spores can be used as advanced materials to encapsulate a wide variety of pharmaceutical drugs, chemicals, cosmetics, and food supplements. Here, for the first time a strategy to utilize natural spores as advanced materials is developed to encapsulate macromolecules by three different microencapsulation techniques including passive, compression, and vacuum loading. The natural spore formulations developed by these techniques are extensively characterized with respect to size uniformity, shape, encapsulation efficiency, and localization of macromolecules in the spores. In vitro release profiles of developed spore formulations in simulated gastric and intestinal fluids have also been studied, and alginate coatings to tune the release profile using vacuum‐loaded spores have been explored. These results provide the basis for further exploration into the encapsulation of a wide range of therapeutic molecules in natural plant spores.

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“…A previous study verified that the biostructure of this scaffold was very similar to natural human bone with both porosity and perfect biodegradability, which should make it possible for the scaffold to become an ideal antibiotics carrier for the repair of infected bony defects. [ 1 ] Although it was theoretically demonstrated that all of the scaffold monomers and degradation products had no significant toxicity or side effects to the human body,[ 2 3 ] the scaffold would undergo changes and reactions during procedures including fabrication, storage, and sterilization. Furthermore, the blood concentration of the loaded antibiotics could reach toxic levels at certain times, especially in the early stages after implantation.…”

Section: Introductionmentioning

confidence: 99%

Biosafety of the Novel Vancomycin-loaded Bone-like Hydroxyapatite/Poly-amino Acid Bony Scaffold

Cao

Jiang

Yan

et al. 2016

Chinese Medical Journal

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Background:Recently, local sustained-release antibiotics systems have been developed because they can increase local foci of concentrated antibiotics without increasing the plasma concentration, and thereby effectively decrease any systemic toxicity and side effects. A vancomycin-loaded bone-like hydroxyapatite/poly-amino acid (V-BHA/PAA) bony scaffold was successfully fabricated with vancomycin-loaded poly lactic-co-glycolic acid microspheres and BHA/PAA, which was demonstrated to exhibit both porosity and perfect biodegradability. The aim of this study was to systematically evaluate the biosafety of this novel scaffold by conducting toxicity tests in vitro and in vivo.Methods:According to the ISO rules for medical implant biosafety, for in vitro tests, the scaffold was incubated with L929 fibroblasts or rabbit noncoagulant blood, with simultaneous creation of positive control and negative control groups. The growth condition of L929 cells and hemolytic ratio were respectively evaluated after various incubation periods. For in vivo tests, a chronic osteomyelitis model involving the right proximal tibia of New Zealand white rabbits was established. After bacterial identification, the drug-loaded scaffold, drug-unloaded BHA/PAA, and poly (methyl methacrylate) were implanted, and a blank control group was also set up. Subsequently, the in vivo blood drug concentrations were measured, and the kidney and liver functions were evaluated.Results:In the in vitro tests, the cytotoxicity grades of V-BHA/PAA and BHA/PAA-based on the relative growth rate were all below 1. The hemolysis ratios of V-BHA/PAA and BHA/PAA were 2.27% and 1.42%, respectively, both below 5%. In the in vivo tests, the blood concentration of vancomycin after implantation of V-BHA/PAA was measured at far below its toxic concentration (60 mg/L), and the function and histomorphology of the liver and kidney were all normal.Conclusion:According to ISO standards, the V-BHA/PAA scaffold is considered to have sufficient safety for clinical utilization.

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Preparation and properties of BSA-loaded microspheres based on multi-(amino acid) copolymer for protein delivery

Cited by 18 publications

References 29 publications

Protein Polymer-Based Nanoparticles: Fabrication and Medical Applications

Protein Polymer-Based Nanoparticles: Fabrication and Medical Applications

Lycopodium Spores: A Naturally Manufactured, Superrobust Biomaterial for Drug Delivery

Biosafety of the Novel Vancomycin-loaded Bone-like Hydroxyapatite/Poly-amino Acid Bony Scaffold

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