Preparation and properties of monoclonal antibodies against murine interferon-β

Bosveld, I. J.; Vonk, W. P.; Hekman, R.A.C.P.; Vliet, P.W.Van; Jonge, Peter de; Ewijk, Willem van; Trapman, Jan

doi:10.1016/0042-6822(82)90021-6

Cited by 8 publications

(5 citation statements)

References 21 publications

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“…Recombinant murine IFN-Q (rMuIFN-ß) was derived from CHO-flAA3 cells, also kindly provided by Dr. J. Trapman. The interferon was purified by affinity chromatography on monoclonal anti-IFN-ß (25), giving a preparation of "1083 (range : 10'' 9 -10 8 '8) U/mg protein . rMuIFN--y was derived from the supernatant fluid of Mick cells, aCHO cell line that carries and expresses an amplified murine IFN -'Y cDNA (26).…”

Section: Methodsmentioning

confidence: 99%

Interferon gamma, a mediator of lethal lipopolysaccharide-induced Shwartzman-like shock reactions in mice.

Heremans

Damme

Dillen

et al. 1990

The Journal of Experimental Medicine

201

119

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The involvement of cytokines in the pathogenesis of a generalized, Shwartzman-like lethal inflammatory response to bacterial lipopolysaccharides (LPS) was studied by testing the ability of cytokines or neutralizing anticytokine antibodies to modify the course of the syndrome. The reaction was elicitable in non-SPF NMRI mice by two consecutive injections of S. marcescens LPS: a first injection in the footpad, followed after 24 h by an intravenous dose; the size and route of the preparatory LPS dose were found to be critical. Treatment with mAbs against IFN-gamma was found to completely prevent the reaction. Treatment with IFN-gamma on the other hand, rendered the mice more sensitive to elicitation of the reaction. In contrast, systemic administration of IFN-alpha/beta exerted a desensitizing effect. The role of endogenous cytokines in the pathogenesis of this generalized Shwartzman reaction was also documented by a study of the cytokine levels in the serum of the mice. In comparisons between mice given lethal and nonlethal induction schedules, a good correlation was found between mortality rates and height of IFN or TNF levels, but no correlation was seen with IL-6 levels. Also, in mice that were protected by anti-IFN-gamma antibody, serum IFN and TNF were undetectable, whereas IL-6 levels were as high as in unprotected mice. These data provide evidence that among the cytokines that govern the inflammatory response to LPS, endogenous IFN-gamma occupies a key position. These findings therefore also open perspectives for clinical application of IFN-gamma antagonists.

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Section: Methodsmentioning

confidence: 99%

Interferon gamma, a mediator of lethal lipopolysaccharide-induced Shwartzman-like shock reactions in mice.

Heremans

Damme

Dillen

et al. 1990

The Journal of Experimental Medicine

201

119

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“…The resulting events are thus removed from the spectator and added to the rescattering sample. The spectrum of rescattered spectators can be calculated using a generalization of F x (x, r 0 ) which includes the distribution of the longitudinal momentum p 3 carried by the fragments [29].…”

Section: Spectator Modificationmentioning

confidence: 99%

Slow proton production in deep-inelastic neutrino scattering on deuterium

1994

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The cross section for semi-inclusive deep inelastic charged current neutrino scattering on hydrogen and deuterium in which a slow proton is observed in coincidence with the muon is computed as a function of Bjorken x and light-cone momentum of the detected proton. In the Impulse Approximation contributions from hadronization and (in case of the deuteron) the emission of a spectator nucleon are considered. In addition the probability for rescattering is computed. The results are compared to a recent analysis of the data from the WA25 (BEBC) experiment at CERN. 25.30.Pt,13.85.Hi

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“…We quantitatively separated MulFN-~ from MulFN-fl by affinity chromatography over a monoclonal (EB3) anti-MulFN-fl antibody column. This monoclonal antibody was previously shown to be able to bind MulFN-fl completely but not MulFN-~ (Bosveld et al, 1982;Vonk & Trapman, 1983).…”

Section: Gel Filtration Of Mulfn-~mentioning

confidence: 98%

Isolation and Characterization of Subspecies of Murine Interferon Alpha

Lemson¹,

Vonk²,

Korput³

et al. 1984

Journal of General Virology

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SUMMARYInterferon produced by mouse L-929 cells by incubation with poly(rI), poty(rC) is known to be composed of a mixture of MuIFN-c~ and MuIFN-fl. The c~ component was separated from the fl species by affinity chromatography over a monoclonal antiMuIFN-fl agarose column and partially purified by gel filtration. MuIFN-c~, prepared by this method was separated into at least five subspecies by chromatofocusing. The approximate pI values of these components are >~ 7.5, 6.5, 6.2, 5-9 and 5.6, respectively. Component 3 (pI 6.2) was the most prominent subspecies present in our MulFN-~ preparations, representing 40 to 50 ~o of the total antiviral activity. Component 1 (pI /> 7.5) which accounted for about 5~ of the antiviral activity on mouse cells, differed in some properties from the other interferon subspecies. It showed a relatively high antiviral activity on heterologous cells and it was eluted from a Sephadex column after the other c¢ subspecies. Furthermore, it showed a diminished binding to heparin as compared to the other MulFN-c¢ subspecies, indicating a lower affinity for polynucleotides.

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Preparation and properties of monoclonal antibodies against murine interferon-β

Cited by 8 publications

References 21 publications

Interferon gamma, a mediator of lethal lipopolysaccharide-induced Shwartzman-like shock reactions in mice.

Interferon gamma, a mediator of lethal lipopolysaccharide-induced Shwartzman-like shock reactions in mice.

Slow proton production in deep-inelastic neutrino scattering on deuterium

Isolation and Characterization of Subspecies of Murine Interferon Alpha

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