Preparation and properties of superparamagnetic nanoparticles with narrow size distribution and biocompatible

Jiang, Wei Teh; Yang, Hong-Chang; Yang, Shieh‐Yueh; Horng, Herng-Er; Hung, J.; Chen, Yang Ching; Hong, Chin Yih

doi:10.1016/j.jmmm.2004.05.022

Cited by 244 publications

(113 citation statements)

References 19 publications

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“…It may be due to the formation of monolayer coverage of magnetite was reached in the presence of Ethylene di aminetetra acetic acid (EDTA) by coordinating-FeOH sites via one or two of their carboxylate functionalities through a water bridge with an outer sphere chemisorptions complexation. It is a good agreement with previous reports related to the addition of citrate ions in the coprecipitation solution of Massart process allowed for a decrease in the diameter of citrate-coated nanoparticles from 8 to 3 nm [23][24][25]. …”

Section: Synthesis Of Nanoparticlessupporting

confidence: 92%

Co-Precipitation and Magnetic Properties of Magnetite Nanoparticles for Potential Biomedical Applications

Daoush¹

2017

JNMR

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Magnetite nanopowder was synthesized by co-deposition of iron salts in the presence of ethylenediaminetetraacetic acid (EDTA) using sodium hydroxide as a precipitating agent. The iron chloride underwent complex formation reaction with (EDTA) followed by co-precipitation of the iron complex by boiling to produce the magnetite powder. The prepared powders were investigated by high magnification SEM and XRD to identify the particle shape and size of the obtained magnetite nanoparticles and its chemical composition. It was observed that the produced magnetite powder has spherical like particle shape with ~30 nm particle sizes and face centered cubic crystal structure. The magnetic properties of the obtained powder were measured using vibrating sample magnetometry (VSM). It was revaled that the obtained nanomagnetite particles have super magnetic character which of saturation magnetization 88.1 emu/g and squarness ratio of 0.14 which has the ability to used as a contrasting agent in magneto resonance imaging (MRI) diagnosis.

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Section: Synthesis Of Nanoparticlessupporting

confidence: 92%

Co-Precipitation and Magnetic Properties of Magnetite Nanoparticles for Potential Biomedical Applications

Daoush¹

2017

JNMR

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“…Dextran was oxidized to generate aldehyde groups (-CHO) to biofunctionalize the magnetic nanoparticles with antibodies against hCGβ, and then it responded to anti-hCGβ with the dextran aldehyde groups to form a covalent bond (-CH=N-), thus yielding the magnetic reagent to detect hCGβ concentrations. 22 The size distribution of the magnetic nanoparticles was analyzed by laser dynamic scattering, and the mean diameter was found to be 52.8 nm ( Figure 2B). Through magnetic separation, unbound anti-hCGβ was detached from the solution, and anti-hCGβ biofunctionalized magnetic nanoparticles were disseminated in the PBS solution.…”

Section: Preparation Of Magnetic Reagentsmentioning

confidence: 99%

Quantitative analysis of total β-subunit of human chorionic gonadotropin concentration in urine by immunomagnetic reduction to assist in the diagnosis of ectopic pregnancy

Chen

Hwu

Chang

2015

IJN

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Background The initial diagnosis of ectopic pregnancy depends on physical examination, ultrasound, and serial measurements of total β-subunit of human chorionic gonadotropin (hCGβ) concentrations in serum. The aim of this study was to explore the possibility of using quantitative analysis of total hCGβ in urine rather than in serum by immunomagnetic reduction (IMR) assay as an alternative method to diagnose an ectopic pregnancy. Methods We established a standard calibration curve of IMR intensity against total hCGβ concentration based on standard hCGβ samples, and used an IMR assay to detect total hCGβ concentrations in the urine of pregnant women with lower abdominal pain and/or vaginal bleeding. The final diagnosis of ectopic pregnancy was based on ultrasound scans, operative findings, and pathology reports. In this prospective study, ten clinical samples were used to analyze the relationship of total hCGβ IMR signals between urine and serum. Furthermore, 20 clinical samples were used to analyze the relationship between urine IMR signals and serum levels of total hCGβ. Results The calibration curve extended from 0.01 ng/mL to 10,000 ng/mL with an excellent correlation ( R 2 =0.999). In addition, an excellent correlation of total hCGβ IMR signals between urine and serum was noted ( R 2 =0.994). Furthermore, a high correlation between urine IMR signals and serum levels of total hCGβ was noted ( R 2 =0.862). Conclusion An IMR assay can quantitatively analyze total hCGβ concentrations in urine, and is a potential candidate for point-of-care testing to assist in the diagnosis of ectopic pregnancy.

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“…[32][33][34] Briefly, a ferrite solution containing ferrous sulfate heptahydrate (FeSO 4 ⋅7H 2 O) and ferric chloride hexahydrate (FeCl 3 ⋅6H 2 O) in a stoichiometric ratio of 1:2 was mixed with an equal volume of aqueous dextran. Dextran was served as a surfactant for Fe 3 O 4 particles distributed in water because of its nontoxicity, hydrophilicity, and high affinity for biological macromolecules.…”

Section: Conjugation Of Cd133 Ab With Uspiomentioning

confidence: 99%

Specific detection of CD133-positive tumor cells with iron oxide nanoparticles labeling using noninvasive molecular magnetic resonance imaging

Chen¹,

Liou²,

Pan³

et al. 2015

IJN

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Background The use of ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles to visualize cells has been applied clinically, showing the potential for monitoring cells in vivo with magnetic resonance imaging (MRI). USPIO conjugated with anti-CD133 antibodies (USPIO-CD133 Ab) that recognize the CD133 molecule, a cancer stem cell marker in a variety of cancers, was studied as a novel and potent agent for MRI contrast enhancement of tumor cells. Materials and methods Anti-CD133 antibodies were used to conjugate with USPIO via interaction of streptavidin and biotin for in vivo labeling of CD133-positive cells in xenografted tumors and N -ethyl- N -nitrosourea (ENU)-induced brain tumors. The specific binding of USPIO-CD133 Ab to CD133-positive tumor cells was subsequently detected by Prussian blue staining and MRI with T2-weighted, gradient echo and multiple echo recombined gradient echo images. In addition, the cellular toxicity of USPIO-CD133 Ab was determined by analyzing cell proliferation, apoptosis, and reactive oxygen species production. Results USPIO-CD133 Ab specifically recognizes in vitro and labels CD133-positive cells, as validated using Prussian blue staining and MRI. The assays of cell proliferation, apoptosis, and reactive oxygen species production showed no significant differences in tumor cells with or without labeling of USPIO-CD133 Ab. In vivo imaging of CD133-positive cells was demonstrated by intravenous injection of USPIO-CD133 Ab in mice with HT29 xenografted tumors. The MRI of HT29 xenografts showed several clusters of hypotensive regions that correlated with CD133 expression and Prussian blue staining for iron. In rat, brain tumors induced by transplacental ENU mutagenesis, several clusters of hypointensive zones were observed in CD133-expressing brain tumors by MRI and intravenously administered USPIO-CD133 Ab. Conclusion Combination of USPIO-CD133 Ab and MRI is valuable in recognizing CD133-expressing tumor cells in vitro, extracellularly labeling for cell tracking and detecting CD133-expressing tumors in xenografted tumors as well as ENU-induced rat brain tumors.

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Preparation and properties of superparamagnetic nanoparticles with narrow size distribution and biocompatible

Cited by 244 publications

References 19 publications

Co-Precipitation and Magnetic Properties of Magnetite Nanoparticles for Potential Biomedical Applications

Co-Precipitation and Magnetic Properties of Magnetite Nanoparticles for Potential Biomedical Applications

Quantitative analysis of total β-subunit of human chorionic gonadotropin concentration in urine by immunomagnetic reduction to assist in the diagnosis of ectopic pregnancy

Specific detection of CD133-positive tumor cells with iron oxide nanoparticles labeling using noninvasive molecular magnetic resonance imaging

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Preparation and properties of superparamagnetic nanoparticles with narrow size distribution and biocompatible

Cited by 244 publications

References 19 publications

Co-Precipitation and Magnetic Properties of Magnetite Nanoparticles for Potential Biomedical Applications

Co-Precipitation and Magnetic Properties of Magnetite Nanoparticles for Potential Biomedical Applications

Quantitative analysis of total &beta;-subunit of human chorionic gonadotropin concentration in urine by immunomagnetic reduction to assist in the diagnosis of ectopic pregnancy

Specific detection of CD133-positive tumor cells with iron oxide nanoparticles labeling using&nbsp;noninvasive molecular magnetic resonance&nbsp;imaging

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Quantitative analysis of total β-subunit of human chorionic gonadotropin concentration in urine by immunomagnetic reduction to assist in the diagnosis of ectopic pregnancy

Specific detection of CD133-positive tumor cells with iron oxide nanoparticles labeling using noninvasive molecular magnetic resonance imaging