Preparation and structural analysis of Langmuir-Blodgett films of acidic and zwitterionic phospholipids

Lohner, Karl; Konovalov, Oleg; Samoilenko, I. I.; Myagkov, I. V.; Troitzky, V. I.; Berzina, T. I.

doi:10.1016/s0040-6090(96)08794-9

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…Cholesterol has, moreover, a stabilizing effect on the monolayers. The stabilizing effect of cholesterol has also been demonstrated with vesicles, black lipid membranes, , and DPPC monolayers. , …”

Section: Resultsmentioning

confidence: 95%

Oriented Immobilization of Antibodies for Immunosensing

and

Albers

1998

Langmuir

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The covalent coupling of antibody fragments to linkers embedded in a monolayer matrix of phosphatidylcholine and cholesterol was examined at the air-water interface by the means of a quartz crystal microbalance, QCM. Two linkers that bind the free thiols of the Fab′ fragment were investigated. The nonspecific binding of bovine serum albumin and the specific binding of antigen were also monitored with the QCM. Standardized radioimmunoassay was used to confirm the immunoreaction and determine binding parameters. The monolayer formation of the linker lipids in the ternary system of phosphatidylcholine and cholesterol was, moreover, demonstrated by film balance studies. The results demonstrate that the covalent coupling of Fab′ fragments to linking groups embedded in a phospholipid monolayer matrix is a promising approach to achieve a defined immobilization of antibodies at the sensor surface with high antigen binding effeciency.

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Section: Resultsmentioning

confidence: 95%

Oriented Immobilization of Antibodies for Immunosensing

and

Albers

1998

Langmuir

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“…The polar region density was calculated considering a few different models with different Zn−DLPA ratios (from 0.5:1 to 1:1) and different hydration shells. The length of the hydrocarbon chain in the all-trans configuration was estimated to be 1.38 nm from the equation where n is the number of CH 2 groups, 0.125 nm is the contribution to the chain length of each CH 2 group, and 0.1265 nm is the contribution of the terminal CH 3 group …”

Section: Resultsmentioning

confidence: 99%

“…The fit of DMPC monolayer reflectivity data was performed by assuming a 1:1:5 (Zn/DMPC/H 2 O) complex, as suggested for 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC) that has the same polar group . The hydrophobic region thickness was 1.6265 nm from eq 5, and the polar region thickness was 1.0235 nm from the dipalmitoyl-phosphatidylcholine (DPPC) data given by Lohner et al…”

Section: Resultsmentioning

confidence: 99%

Total External Reflection X-ray Absorption Spectroscopy Reveals a Zinc Coordination Shell in Phospholipid Langmuir−Blodgett Films

et al. 2002

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We have studied the local environment of zinc in phospholipid Langmuir−Blodgett films with extended X-ray absorption fine structure spectroscopy in total reflection conditions (reflEXAFS). Mono- and multilayer films made of dilauroyl-phosphatidic acid (DLPA) and monolayer films made of dimyristoyl-phosphatidylcholine (DMPC) were deposited on quartz slides from ZnCl2 solutions, and their molecular order was checked by means of X-ray reflectivity measurements. A very good signal-to-noise ratio in the reflEXAFS spectra of both multi- and monomolecular films was obtained. The theoretical fits to the experimental data show that zinc coordinates four atoms about 0.20 nm distant in both acidic DLPA and neutral DMPC films, while in water solutions, zinc coordinates six atoms about 0.21 nm distant.

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Application of Neutron Reflectivity for Studies of Biomolecular Structures and Functions at Interfaces

Johs

Liang

et al. 2009

Neutron Applications in Earth, Energy and Environmental Sciences

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Preparation and structural analysis of Langmuir-Blodgett films of acidic and zwitterionic phospholipids

Cited by 6 publications

References 29 publications

Oriented Immobilization of Antibodies for Immunosensing

Oriented Immobilization of Antibodies for Immunosensing

Total External Reflection X-ray Absorption Spectroscopy Reveals a Zinc Coordination Shell in Phospholipid Langmuir−Blodgett Films

Application of Neutron Reflectivity for Studies of Biomolecular Structures and Functions at Interfaces

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