Preparation and study of starch/ collagen/ polycaprolactone nanofiber scaffolds for bone tissue engineering using electrospinning technique

Abstract

Bone tissue engineering has been a fast growing area recently. It suggests a novel and talented method for bone regeneration. The aim of the current work is to produce nanofiber scaffolds based on starch/ collagen/ polycaprolactone (PCL) biomaterials by means of an electrospinning methodology for bone tissue engineering purposes. The results showed that the developed structures have good physicochemical and interconnected properties that could be considered for bone tissues engineering. The results from the characterization specified that the nanofibers were successfully prepared with monodispersed nanosized diameter (60 nm), uniform network shaped morphology and negative surface charge (-13.5 mV). Besides, the applied method can be set up to prepare fiber-based structures using other polymeric materials. We believe that by incorporating different materials to reduce the degradation rate of the fibers, it can be matched with the speed of tissue regeneration. In this case, the prepared nanofibers can be used as the membrane biomaterials, for example, guided bone regeneration (GBR) membrane.

The Collagen-Based Scaffolds for Bone Regeneration: A Journey through Electrospun Composites Integrated with Organic and Inorganic Additives

“…To note, the cells were not packed on the PCL single membrane, while packed cell growth was observed in the hybrid structure. Other attempts at designing collagen-based electrospun composites by assisting the PCL polymer could be found in Baylan et al [76], Choi et al [77], Kim and Kim [78], and Ghavimi et al [79].…”

“…To note, the cells were not packed on the PCL single membrane, while packed cell growth was observed in the hybrid structure. Other attempts at designing collagenbased electrospun composites by assisting the PCL polymer could be found in Baylan et al [76], Choi et al [77], Kim and Kim [78], and Ghavimi et al [79]. .05 was taken to be statistically significant, showing significant increase in ALP activity on PCD (** p = 0.0040) and a further increment on PCDS (*** p = 0.0002), Reproduced from reference [74] with permission from Elsevier.…”

Gelatin-hydroxyapatite Fibrous Nanocomposite for Regenerative Dentistry and bone Tissue Engineering

“…Processing variables for the electrospinning procedure including voltage, the distance of needle-to-collector, flow rate, as well as parameters of solution such as viscosity, the electrical conductivity of the solution, and surface tension can control the morphology of nanofibers [29]. The purpose of optimizing the electrospinning method is to identify the conditions for preparing nanofibers with a minimum diameter [31]. The results of other researchers * * displayed that polymeric nanoparticles, lipid nanoparticles, micelles, nanotubes, etc.…”

Effect of Dehydrothermal Treatment on the Structure and Properties of a Collagen-Based Heterogeneous Bilayer Membrane

“…Collagen is the most commonly used membrane raw material, and collagen-based resorbable barrier membranes have been researched on GBR application since the late 1980s. , As one of the abundant and natural biopolymer materials, collagen has plenty of prominent physical and chemical properties, which are advantageous for preparing biomaterials, including affinity for metal ions, good biocompatibility, low immunogenicity, flexibility, unique triple-helix structure, and blood coagulation performance. Type I collagen is the main component of extracellular matrix (ECM), accounting for more than 80% of all types of collagen, which has been used to accelerate wound healing and tissue regeneration as biomaterials. ,, In particular, type I collagen has performed a vital role in application of bone tissue engineering. Although collagen membrane has a higher biological activity than synthetic substitutes, it may collapse at bone defects due to poor mechanical properties and rapid degradation, resulting in insufficient space for bone regeneration .…”

“…Type I collagen is the main component of extracellular matrix (ECM), accounting for more than 80% of all types of collagen, 19 which has been used to accelerate wound healing and tissue regeneration as biomaterials. 15,20,21 In particular, type I collagen has performed a vital role in application of bone tissue engineering. Although collagen membrane has a higher biological activity than synthetic substitutes, it may collapse at bone defects due to poor mechanical properties and rapid degradation, resulting in insufficient space for bone regeneration.…”