Preparation and use of biotinylated ligands for LHRH receptor purification

Quantitative evaluation of the lengths of homobifunctional protein cross‐linking reagents used as molecular rulers

“…The cross-linking spans of disuccinimidyl glutarate (DSG; e.g., see Eisman and Schnaare 1996;Horiguchi et al 1997;Pan et al 1998;Holwerda 1999;Xu et al 1999;Korsgren et al 2000), dithiobis(succinimidylpropionate) (DSP; e.g., see Walleczek et al 1989;Meunier et al 1991;Poruchynsky and Atkinson 1991;Jansson et al 1996;Stout and Kirley 1996;Tanaka et al 1996;Carl et al 1998), disuccinimidyl suberate (DSS; e.g., see Chain and Malkin 1991;Pliszka 1993;Persson and Ezban 1994;Loester et al 1995;Stout and Kirley 1996;Carl et al 1998;Leonard et al 1998), disuccimimidyl tartarate (DST; e.g., see Bragg and Hou 1986;Farries et al 1988;Mita et al 1989;Miyazaki et al 1990;Plouet and Moukadiri 1990;Amiranoff and Lori- Chemicals (1999). net-Laburthe 1991; Chen et al 1992;Maman et al 1994;Horiguchi et al 1997), ethyleneglycol bis-(succinimidylsuccinate) (EGS; e.g., see Huey and Hugli 1985;Bladon et al 1989;Caplow and Shanks 1990;Geisler et al 1992), and bis(2-[succinimidooxycarbonyloxy]ethyl)sulfone (BSOCOES; e.g., see Smith et al 1986;Svoboda et al 1988aSvoboda et al , 1988bSchoffelmeer et al 1989;…”