Preparation and use of fibrin glue in surgery

Silver, Frederick H.; Wang, Ming Che; Pins, George D.

doi:10.1016/0142-9612(95)93113-r

Cited by 141 publications

(101 citation statements)

References 69 publications

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“…Fibrin gel was used as the cell entrapment medium (-2 mgkomposite construct). The cells (-250,000 pl-') were resuspended in fully supplemented medium (FSM = Dubelcco's modified Eagle medium (DMEM) containing 10% fetal bovine serum, 4.5 g/l glucose, 10 mM N-2-hydroxyethylpiperazine N'-2-ethane sulfonic acid (Hepes), 0.1 mM non-essential amino acids, 0.4 mM proline, 50 mg/l ascorbic acid, 100 Ulml penicillin, 100 pg/ml streptomycin, and 0.5 pg/ ml fungizone) containing bovine thrombin (1 :20 dilution of 1000 units/ ml, Johnson & Johnson, Arlington, TX) and mixed with an equal volume of bovine fibrinogen (100 mg/ml) (Sigma Chemical, St. Louis, Resenrch 20 (2002) [16][17][18][19] 17 MO) in saline solution. The polymer non-woven mesh was made from PGA (2 mm x 5 mm diameter, 60 mglml, 97% porous from Albany International Research, Mansfield, MA).…”

Section: Coniposite Fabricationmentioning

confidence: 99%

A biodegradable composite scaffold for cell transplantation

Mahmood

Langer

2002

Journal Orthopaedic Research

157

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Cell transplantation is rapidly becoming a therapeutic option to treat disease and injury. However, standard techniques for cell seeding on non-woven polymer meshes or within gels may not be suitable for immediate implantation or surgical manipulations of freshly isolated cells. Therefore, a biodegradable composite system was developed as a way to rapidly entrap cells within a support of predefined shape to potentially facilitate cell delivery into a target site (e.g. meniscal tears in the avascular zone). The composite construct consisted of freshly isolated cells, in this case pig chondrocytes, entrapped in a fibrin gel phase and dispersed throughout the void volume of a polyglycolic acid (PGA) non-woven mesh. Composites were cultured for up to 4 weeks. In vitro degradation of fibrin gel was evaluated via gel-entrapped urokinase. At 28 days in culture, glycosaminoglycan (GAG) content per cell in the composite scaffolds was 2.6 times that of the PGA-only cell construct group and 88% that of native pig cartilage. Total collagen content per cell in the composite scaffolds was not significantly different from the PGA-only cell construct group (P > 0.02) and

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Section: Coniposite Fabricationmentioning

confidence: 99%

A biodegradable composite scaffold for cell transplantation

Mahmood

Langer

2002

Journal Orthopaedic Research

157

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“…Nowadays, the most used surgical adhesives are based on fibrin [3,4] and cyanoacrylates [5,6]. The fibrin based adhesives present several problems, e.g.…”

Section: Introductionmentioning

confidence: 99%

Modification of the biopolymer castor oil with free isocyanate groups to be applied as bioadhesive

Ferreira

Pereira

Coelho

et al. 2007

International Journal of Biological Macromolecules

133

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Surgical adhesives have been used for several applications, including haemostasis, sealing air leakages and tissue adhesion. The aim of this work was to develop a biodegradable urethane-based bioadhesive containing free isocyanate groups. This material presents the advantage of being biodegradable, biocompatible and having the capacity of reacting with amino groups present in the biological molecules.A urethane based on castor oil (CO) was synthesized by reaction of the molecule with isophorone diisocyanate (IPD). The characterization of the material was accomplished by different techniques: ATR-FT-IR (attenuated transmittance reflection-Fourier transform infrared), swelling capacity determination, evaluation of the moisture curing kinetics, reaction with aminated substrates and determination of surface energy by contact angle measurement. The study of the urethane thermal properties was performed by DMTA (dynamical mechanical thermal analysis) and TGA (thermogravimetric analysis). The haemocompatibility of the urethane was also evaluated by thrombosis and haemolysis tests.

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“…Entretanto, o uso da cola reduziu o número de suturas e conseqüentemente o tempo cirúrgico (Saltz et al, 1991;Silver et al, 1995;Stolf, 1998), fato bastante significativo em pacientes de alto risco.…”

Section: Discussionunclassified

“…Aplicada no enxerto e em suas margens, auxilia tanto na fixação deste como na hemostasia da ferida. Ela tem sido usada para eliminar o espaço morto e aumentar a probabilidade de o enxerto ser revascularizado e diminuir o número de suturas em cirurgias cosméticas e reconstrutivas (Silver et al, 1995).…”

Section: Introductionunclassified

Fixação de enxerto cutâneo em malha de espessura total com sutura ou cola de fibrina

Amaral

Rahal

Dal-Pai

et al. 2004

Arq. Bras. Med. Vet. Zootec.

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RESUMOAvaliou-se a influência da cola de fibrina, derivada do veneno de serpente, na fixação e integração de enxerto de pele. Foram utilizados nove cães, sem raça definida, com peso médio de 15kg. Foi induzida ferida de 4×4cm de área, na face crânio-proximal dos antebraços direito e esquerdo. Um enxerto de espessura total foi colhido da região torácica e expandido por meio de expansor de pele. No membro direito o enxerto foi estabilizado no leito receptor por meio de pontos isolados simples; no esquerdo foi fixado pela aplicação de cola de fibrina e oito pontos de sutura. O sítio doador foi fechado empregando-se retalho cutâneo bipediculado. As bandagens do leito receptor foram oclusivas e não aderentes e aplicou-se pomada de neomicina com bacitracina. A troca de bandagens ocorreu diariamente até o sétimo dia de pós-operatório e, posteriormente, a cada três dias. A área de sobrevivência do enxerto foi obtida pela subtração das áreas não viáveis e total medidas com fotomicroscópio Nikon conectado a um sistema de análise de imagem KS-300 aos três, sete, 15 e 30 dias de pós-operatório. Para a avaliação microscópica, a área do enxerto foi colhida em três animais aos sete, 15 e 30 dias de pós-operatório. Não houve diferença entre momentos de avaliação e técnicas de fixação quanto à área de enxerto viável. Os enxertos fixados com cola apresentaram estágio de reparação mais avançado em todos os momentos. Concluiu-se que a cola de fibrina derivada do veneno de serpente tem moderado poder adesivo e, pela análise microscópica, favorece a integração do enxerto cutâneo em malha de espessura total.Palavras-chave: cola de fibrina, enxerto cutâneo, sutura, serpente ABSTRACT

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Preparation and use of fibrin glue in surgery

Cited by 141 publications

References 69 publications

A biodegradable composite scaffold for cell transplantation

A biodegradable composite scaffold for cell transplantation

Modification of the biopolymer castor oil with free isocyanate groups to be applied as bioadhesive

Fixação de enxerto cutâneo em malha de espessura total com sutura ou cola de fibrina

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