Preparation and utilization of fluorescent synthetic peptides

Site-specific labeling of synthetic peptide using the chemoselective reaction betweenN-methoxyamino acid and isothiocyanate

“…In order to find the optimum condition for the specific labeling of N ‐methoxyamino group, a nonapeptide carrying MeOGly at its N ‐terminus, MeOGly‐Gly‐Leu‐Glu‐Phe‐Lys‐Ala‐Gly‐Arg‐NH 2 , 1 was used as a model. For the introduction of fluorescent probe, an isothiocyanate of corresponding fluorophore has often been used. Thus, we first examined the reaction of peptide 1 with PITC as the model reagent.…”

Fluorescent Labeling of Biomolecules with Organic Probes

“…Chersie and collaborators used dansyl chloride (54, Figure 7) for direct "in synthesis" labeling of peptides. 81,82 More recently, several research studies were published relating to analysis of amino acids in biological samples through the HPLC technique (λ ) 286 or 254 nm) using this compound as a precolumn derivatization reagent. 83,84 Fluorescently labeled proteins are useful in a large number of bioanalytical applications including in vivo imaging, highthroughput screening, diagnostics, proteomics, and singlebiomolecule spectroscopy.…”

“…Chersie and collaborators used dansyl chloride ( 54 , Figure ) for direct “in synthesis” labeling of peptides. , More recently, several research studies were published relating to analysis of amino acids in biological samples through the HPLC technique (λ = 286 or 254 nm) using this compound as a precolumn derivatization reagent. , …”

Overlapping synthetic peptides as a tool to map protein-protein interactions ̶ FSH as a model system of nonadditive interactions

“…The results suggested the presence of several regions of interaction. The 125 I-FSH-α subunit was able to bind primarily to the synthetic peptides FSH-β- (11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25), (41)(42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53)(54)(55), (51)(52)(53)(54)(55)(56)(57)(58)(59)(60)(61)(62)(63)(64)(65), (101)(102)(103)(104)(105)…”

“…Other laboratories later improved this initial solid-phase approach, and at present, the peptides can be labeled with fluorochromes, biotin, etc. for quantification, and attached to the membrane by using linkers or BSA, or even custom made on chips, avoiding the above-mentioned difficulties by using a variety of different strategies [39][40][41][42][43][44][45][46][47] .…”