Preparation, characterization and antibacterial activities of chitosan, N-trimethyl chitosan (TMC) and N-diethylmethyl chitosan (DEMC) nanoparticles loaded with insulin using both the ionotropic gelation and polyelectrolyte complexation methods

Sadeghi, A.M.M.; Dorkoosh, Farid Abedin; Avadi, Mohammad Reza; Saadat, Paria; Rafiee-Tehrani, Morteza; Junginger, Hans E.

doi:10.1016/j.ijpharm.2007.11.052

Cited by 215 publications

(114 citation statements)

References 22 publications

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“…en, according to Sadeghi et al [33], polyelectrolyte complexation reveals that not all anionic polymer charges were balanced, probably due to the low concentration of chitosan. e addition of cationic electrolyte (such as in the combination of methods) decreases the magnitude of negative charges and suggests an increased molecular interaction for the resulting zeta potential values that are close to zero.…”

Section: Zeta Potential Of Hydrogelsmentioning

confidence: 96%

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Polyelectrolyte Complexation versus Ionotropic Gelation for Chitosan-Based Hydrogels with Carboxymethylcellulose, Carboxymethyl Starch, and Alginic Acid

Henao

Delgado

Contreras

et al. 2018

International Journal of Chemical Engineering

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e preparation of gels by charge interaction methods has been extensively studied, but it is not yet clear how these methods influence gel characteristics. e objective of this work was to study differences in morphology and surface charge of hydrogels prepared by ionotropic gelation, polyelectrolyte complexation, and a combination of both methods. us, the anionic charge was provided by carboxymethylcellulose (CMC), carboxymethylated starch (CMS), and alginic acid (AA); calcium chloride (CaCl 2 ) and chitosan (CS) were used for the ionotropic gelation and polyelectrolyte complexation, respectively. ose materials are commercially available, have low toxicity, and are widely used in the area. ese compounds interact through physical crosslinks, which are affected by physical changes of the medium. Our results showed that these two methods produced changes in the morphology of the hydrogels. CMC gels exhibited larger pores in the presence of CaCl 2 . In polyelectrolyte complexation, CMS produced an increased agglomeration of particles, while the addition of CaCl 2 to AA generated dispersed particles of size in the order of millimeters. Mixing both ionotropic gelation and polyelectrolyte complexation methods yielded gels of varied charge (568 mV for CMC, 502 mV for CMS, and 1713 mV for AA). FTIR spectra of the hydrogels showed interactions between the different polymeric compounds, being the greatest changes between 1250 and 1600 cm −1 , due possibly to the replacement of Na by Ca at crosslinking points. erefore, the method of gel preparation employed had a major influence on the size and pore distribution, parameters which in turn influence encapsulation and drug delivery in these systems.

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Section: Zeta Potential Of Hydrogelsmentioning

confidence: 96%

“…Sadeghi et al [33] suggested that zeta potential is an indicator of charge, which is available on the gel surface, and the values reflect the charge that is not neutralized.…”

Section: Zeta Potential Of Hydrogelsmentioning

confidence: 99%

Polyelectrolyte Complexation versus Ionotropic Gelation for Chitosan-Based Hydrogels with Carboxymethylcellulose, Carboxymethyl Starch, and Alginic Acid

Henao

Delgado

Contreras

et al. 2018

International Journal of Chemical Engineering

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“…Recently, CS-NPs were prepared from newly synthesised diethylmethyl CS (DEMC) and TMC by ionotropic gelation method or by PEC (73). Similarly, another group reported the preparation of NPs from newly synthesised tri-ethyl chitosan (TEC) and dimethylethyl chitosan (DMEC) (74,75).…”

Section: Quaternized Derivatives Of Chitosanmentioning

confidence: 99%

“…This was because the positive charge available on the surface of the NPs decreased, and hence, the particles were unable to open the tight junctions of the Caco-2 cell monolayer. In addition, TMC and DEMC in the free form exhibited higher antibacterial properties when compared to the nanoparticulate form (73). Thus, the question arises whether QCS itself is more suitable for oral delivery of proteins and peptides rather than QCS-NPs.…”

Section: Quaternized Derivatives Of Chitosanmentioning

confidence: 99%

Recent Advancement of Chitosan-Based Nanoparticles for Oral Controlled Delivery of Insulin and Other Therapeutic Agents

2010

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Abstract. Nanoparticles composed of naturally occurring biodegradable polymers have emerged as potential carriers of various therapeutic agents for controlled drug delivery through the oral route. Chitosan, a cationic polysaccharide, is one of such biodegradable polymers, which has been extensively exploited for the preparation of nanoparticles for oral controlled delivery of several therapeutic agents. In recent years, the area of focus has shifted from chitosan to chitosan derivatized polymers for the preparation of oral nanoparticles due to its vastly improved properties, such as better drug retention capability, improved permeation, enhanced mucoadhesion and sustained release of therapeutic agents. Chitosan derivatized polymers are primarily the quaternized chitosan derivatives, chitosan cyclodextrin complexes, thiolated chitosan, pegylated chitosan and chitosan combined with other peptides. The current review focuses on the recent advancements in the field of oral controlled release via chitosan nanoparticles and discusses about its in vitro and in vivo implications.

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“…Optimisation of the structure of these polymers for mucosal drug delivery often necessitates modification of the polymer backbone. This may involve processes like trimethylation of primary amine groups (quaternisation) which addresses the pH-dependent solubility of polymers like chitosan while also stabilising the positive surface charge of polycations [11,12,13]. Thiolation of these polymers, which involves the immobilisation of reactive thiol groups on the primary amine groups of the polymer has also been reported to enhance mucoadhesion; thiol groups can oxidise to the reactive thiolate anion S -above pH 5 initiating thiol-disulphide interactions with cysteine-rich subdomains of mucin glycoproteins [14,15,16].…”

mentioning

confidence: 99%

Synthesis, characterisation and in vitro evaluation of novel thiolated derivatives of polyallylamine and quaternised polyallylamine

2015

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Preparation, characterization and antibacterial activities of chitosan, N-trimethyl chitosan (TMC) and N-diethylmethyl chitosan (DEMC) nanoparticles loaded with insulin using both the ionotropic gelation and polyelectrolyte complexation methods

Cited by 215 publications

References 22 publications

Polyelectrolyte Complexation versus Ionotropic Gelation for Chitosan-Based Hydrogels with Carboxymethylcellulose, Carboxymethyl Starch, and Alginic Acid

Polyelectrolyte Complexation versus Ionotropic Gelation for Chitosan-Based Hydrogels with Carboxymethylcellulose, Carboxymethyl Starch, and Alginic Acid

Recent Advancement of Chitosan-Based Nanoparticles for Oral Controlled Delivery of Insulin and Other Therapeutic Agents

Synthesis, characterisation and in vitro evaluation of novel thiolated derivatives of polyallylamine and quaternised polyallylamine

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