Preparation, Characterization, and Anticancer Activity of PEGylated Nano Liposomal Loaded with Rutin against Human Carcinoma Cells (HT‐29)

Abstract

The abstract discusses the development of rutin‐loaded nanoliposomes and their anti‐colorectal cancer activity against human carcinoma cells (HT‐29). The study characterizes the nanoliposomes using the thin‐film hydration method and analyzes their size, charge, and polydispersity index. The encapsulation efficiency and drug loading ability of rutin at different concentrations were investigated. The nanoliposomes were found to be stable for up to one month at 4 ºC and showed sustained drug release for up to 24 hours. The anti‐cancer activity of the rutin‐loaded nanoliposomes was found to be concentration‐dependent and significantly improved compared to free rutin. PEGylated nanoliposomes with rutin (1.8 mg/ml) showed the highest encapsulation efficiency and drug loading ability, along with improved selectivity against cancer cells. Overall, the study provides important insights into the potential use of rutin‐loaded nanoliposomes for the treatment of colorectal cancer.

Encapsulation of gingerol into nanoliposomes: Evaluation of in vitro anti‐inflammatory and anti‐cancer activity

“…The average size, charge, and polydispersity index (PDI) of both the free liposomes and 6-gingerol-encapsulated NLs were measured by dynamic light scattering (DLS) on a Zetasizer Nano-ZS (Malvern Instruments Ltd., Malvern, UK). Samples were diluted (1:20) with deionized water and placed onto the specimen holder of a Zeta-sizer for approximately 1 min prior to measurement to allow room temperature equilibrium (Al-Ekaid et al, 2023;Al-Samydai et al, 2021).…”

ULK1 Mediated Autophagy-Promoting Effects of Rutin-Loaded Chitosan Nanoparticles Contribute to the Activation of NF-κB Signaling Besides Inhibiting EMT in Hep3B Hepatoma Cells

“…They have been tested for their anticancer potential against breast and pancreatic cancer, 41,42 management of cerebral ischemia 43 with anti-inflammatory, 44 antibacterial, antioxidant, and photocatalytic activities 45 and had been suggested for possible use in cosmetics and wound healing. 46,47 Although there are very limited reports on the use of rutin-loaded CNPs for cancer therapy, rutin has been successfully loaded on several other nanoparticles including mineral-, lipid-, ionic-liquid, silica-, PEGylated nanoliposome-, solid lipid-, nanoemulsion-, nanocrystal-, keratin-, PLGA-, platinum and palladium-based nanomaterials and on phyto-sterosomes for the treatment of breast, [48][49][50] lung, 51,52 renal, 53 head and neck, 54 colorectal, 31,55 cervical, 56 brain 57 and liver 58,59 cancers at the preclinical level. Other than cytotoxic effects on cancer cells, rutin conjugated with mesoporous silica, nanolipids, nanomagnesia, selenium, PLGA nanoparticles and prepared in the form of nanocrystals have been proven effective for antibacterial, 60,61 antioxidant, 62 photoprotective, 63 anti-epilectic 64 and anti-parasitic 65 activities.…”