Preparation, characterization and antimicrobial activity of chitosan microparticles with thyme essential oil

Abstract

Considering the therapeutic effects of formulations with violate essential oils, development of an available drug delivery system is of great interest, especially assuming the fact that using essential oils as antimicrobial agents is a rather expanded in antimicrobial therapy nowadays. In this work, chitosan microparticles with encapsulated thyme essential oil were prepared by the emulsion cross-linking method. The effect of thyme oil and glutaraldehyde initial concentrations on particle size, morphology, and particle size distribution was investigated. In addition, the influence of these parameters on the encapsulation of thyme oil in chitosan microparticles, concerning thyme oil loadings and encapsulation efficiency was also tested. The particles showed a spherical shape with an average diameter from 4.71?1.42 to 13.65?4.34 m, depending on the concentration of the essential oil and glutaraldehyde that were used. The diameter of microparticles appeared to increase with increasing the thyme essential oil concentration, and decreased with the increase of glutaraldehyde concentration. It was shown that the concentration of glutaraldehyde did not affect the degree of encapsulation, but the increase in the initial concentration of thyme oil increased the degree of encapsulation of this essential oil in chitosan microparticles. All particles containing thyme essential oil, as well as essential oil of thyme itself showed significant antimicrobial activity against Staphylococcus aureus ATCC 25923, Esherichia coli ATCC 25922, Candida albicans ATCC 24433, Enterococcus faecalis ATCC 25929. This study showed a great potential of the use of thyme essential oil as an antimicrobial agent, especially when encapsulated in a drug delivery system with controlled release of the active antimicrobial component.

Nanoencapsulation of thyme essential oil: a new avenue to enhance its protective role against oxidative stress and cytotoxicity of zinc oxide nanoparticles in rats

“…However, for encapsulated thyme oil (ETO), the droplets were placed onto a carbon-coated copper grid to form a thin liquid film and were negatively stained by one drop of uranyl acetate. The excess of staining was removed using filter paper then the film was air-dried before the observation (Pecarski et al 2014 ). The Orius 1000 CCD camera was used for image acquisition (GATAN, Warrendale, PA, USA).…”

Elimination of oxidative stress and genotoxicity of biosynthesized titanium dioxide nanoparticles in rats via supplementation with whey protein-coated thyme essential oil

“…However, for ETEO, the droplets were placed onto a carbon-coated copper grid to form a thin liquid lm and were negatively stained by one drop of uranyl acetate. The excess staining was removed using lter paper then the lm was air-dried before the observation (Pecarski et al, 2014). The image acquisition was done using Orius 1000 CCD camera (GATAN, Warrendale, PA, USA).…”

Evaluating the Antibacterial Activity and Mode of Action of Thymol-Loaded Chitosan Nanoparticles Against Plant Bacterial Pathogen Xanthomonas campestris pv. campestris

“…The antibacterial effect of thymol-loaded chitosan nanoparticles (TCNPs) has been reported against a broad spectrum of Gram-positive and Gram-negative pathogens (Hu et al, 2009). Furthermore, the inhibitory activity of thyme essential oil-loaded chitosan nanoparticles and nanocapsules has been reported against many foodborne bacterial pathogens (Pecarski et al, 2014). These reports interested us in developing active TCNPs and exploring their activity against Xcc, which is a devastating disease in cruciferous vegetables.…”