Preparation, characterization, and efficacy of thermosensitive liposomes containing paclitaxel

Abstract

To increase the anti-tumor activity of paclitaxel (PTX), novel temperature-sensitive liposomes loading paclitaxel (PTX-TSL) were developed. In vitro, characteristics of PTX-TSL were evaluated. The mean particle diameter was about 100 nm, and the entrapment efficiency was larger than 95%. The phase-transition temperature of PTX-TSL determined by differential scanning calorimetry was about 42C. The result of in vitro drug release from PTX-TSL illustrated that release rate at 37 C was obviously lower than that at 42 C. Stability data indicated that the liposome was physically and chemically stable for at least 3 months at À20 C. In vivo study, after three injections with hyperthermia in the xenograft lung tumor model, PTX-TSL showed distinguished tumor growth suppression, compared with non-temperature-sensitive liposome and free drug. The results of intratumoral drug concentration indicated that PTX-TSL combined with hyperthermia delivered more paxlitaxel into the tumor location than the other two paxlitaxel formulations. In summary, PTX-TSL combined with hyperthermia significantly inhibited tumor growth, due to the increased targeting efficiency of PTX to tumor tissues. Such approach may enhance the delivery efficiency of chemotherapeutics into solid tumors.

Recent Advances in Nanoparticle-Based Cancer Drug and Gene Delivery

“…Wang and coworkers reported more PTX release at 42°C than at 37°C. An in vivo study of a xenograft lung tumor model showed enhanced drug release in tumor tissues when hyperthermia occurred, resulting in increased tumor growth suppression, compared with nontemperature-sensitive liposome and free drug treatment (Wang, Zhang, et al, 2016). …”

Thermosensitive Nanosystems Associated with Hyperthermia for Cancer Treatment

“…Iron oxide-based magnetic nanoparticles are extensively investigated in nanomedicine for their biocompatibility, their contrast agent properties, and their ability to generate heat when submitted to an alternating magnetic field [80,81]. Wang et al designed a multiple magnetic hyperthermia-mediated release system for combination therapy using an injectable, biodegradable, and thermosensitive polymeric hydrogel [82]. Wang et al noted that the therapeutic effect of the combination therapy in vivo significantly reduced the tumors and was dependent on the number of cycles of hyperthermia [82].…”

“…Wang et al designed a multiple magnetic hyperthermia-mediated release system for combination therapy using an injectable, biodegradable, and thermosensitive polymeric hydrogel [82]. Wang et al noted that the therapeutic effect of the combination therapy in vivo significantly reduced the tumors and was dependent on the number of cycles of hyperthermia [82].…”

Thermosensitive Liposomes

“…Apart from anthracyclines [65], the drugs bleomycin [55], melphalan [56], placitaxel [57], docetaxel [58], and gemcitabine [59] have been encapsulated into TSL and delivered to tumors, while reducing systemic drug toxicities.…”