Preparation, characterization and evaluation of chitosan macroporous for potential application in skin tissue engineering

Mei, Liqin; Hu, Deyu; Ma, Jianfeng; Wang, Xiping; Yang, Yunzhi; Liu, Jinsong

doi:10.1016/j.ijbiomac.2012.08.004

Cited by 26 publications

(7 citation statements)

References 27 publications

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“…A study was performed in which chitosan was cross-linked with silica particles (SiO2), used as a porogen agent and the extractions from the developed membranes demonstrated no cytotoxicity against L-929 cells 24 hours after the culture. In addition, the macroporous membrane exhibited excellent cellular adhesion and proliferation after 24 and 48 hours of culturing, which is why the developed scaffold might be adequate for skin tissue engineering [ 89 ]. Chitin-based materials have also demonstrated their potential in maintaining and inducing cell phenotypes used in culturing melanocytes, corneal keratinocytes, and skin keratinocytes [ 90 , 91 ].…”

Section: Chitosan and Tissue Engineeringmentioning

confidence: 99%

Chitosan and Its Potential Use as a Scaffold for Tissue Engineering in Regenerative Medicine

Rodríguez-Vázquez

Vega-Ruiz

Ramos-Zúñiga

et al. 2015

BioMed Research International

493

327

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Tissue engineering is an important therapeutic strategy to be used in regenerative medicine in the present and in the future. Functional biomaterials research is focused on the development and improvement of scaffolding, which can be used to repair or regenerate an organ or tissue. Scaffolds are one of the crucial factors for tissue engineering. Scaffolds consisting of natural polymers have recently been developed more quickly and have gained more popularity. These include chitosan, a copolymer derived from the alkaline deacetylation of chitin. Expectations for use of these scaffolds are increasing as the knowledge regarding their chemical and biological properties expands, and new biomedical applications are investigated. Due to their different biological properties such as being biocompatible, biodegradable, and bioactive, they have given the pattern for use in tissue engineering for repair and/or regeneration of different tissues including skin, bone, cartilage, nerves, liver, and muscle. In this review, we focus on the intrinsic properties offered by chitosan and its use in tissue engineering, considering it as a promising alternative for regenerative medicine as a bioactive polymer.

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Section: Chitosan and Tissue Engineeringmentioning

confidence: 99%

Chitosan and Its Potential Use as a Scaffold for Tissue Engineering in Regenerative Medicine

Rodríguez-Vázquez

Vega-Ruiz

Ramos-Zúñiga

et al. 2015

BioMed Research International

493

327

View full text Add to dashboard Cite

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“…Chitosan has a potential use in skin repair and regeneration subsequent to injuries or burns because it could be cross-linked with the silica particles (SiO2) which used as a porogen agent and the extractions from the developed membranes demonstrated no cytotoxicity against L-929 cells 24 h after the culture. In addition, the macroporous membrane exhibited the excellent cellular adhesion and proliferation after 24 and 48 h of culturing [56]. Chitin-based materials had also demonstrated their potential in keeping and exciting the cell phenotypes used in culturing the melanocytes, corneal keratinocytes and skin keratinocytes [57,58].…”

Section: Chitosanmentioning

confidence: 99%

Recent advances on biomedical applications of scaffolds in wound healing and dermal tissue engineering

Bakhshayesh

Annabi

Khalilov

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

193

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The tissue engineering field has developed in response to the shortcomings related to the replacement of the tissues lost to disease or trauma: donor tissue rejection, chronic inflammation and donor tissue shortages. The driving force behind the tissue engineering is to avoid the mentioned issues by creating the biological substitutes capable of replacing the damaged tissue. This is done by combining the scaffolds, cells and signals in order to create the living, physiological, three-dimensional tissues. A wide variety of skin substitutes are used in the treatment of full-thickness injuries. Substitutes made from skin can harbour the latent viruses, and artificial skin grafts can heal with the extensive scarring, failing to regenerate structures such as glands, nerves and hair follicles. New and practical skin scaffold materials remain to be developed. The current article describes the important information about wound healing scaffolds. The scaffold types which were used in these fields were classified according to the accepted guideline of the biological medicine. Moreover, the present article gave the brief overview on the fundamentals of the tissue engineering, biodegradable polymer properties and their application in skin wound healing. Also, the present review discusses the type of the tissue engineered skin substitutes and modern wound dressings which promote the wound healing.

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“…These groups are able to link to inorganic moieties of silica . However, it also has poor mechanical properties and thermal and chemical stability . In order to improve the physical and chemical properties of chitosan, various crosslinking agents can be employed.…”

Section: Introductionmentioning

confidence: 99%

Preparation of chitosan–silica/PCL composite membrane as wound dressing with enhanced cell attachment

Kiadeh

Ghaee

Mashak

et al. 2017

Polymers for Advanced Techs

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In the present study, the asymmetrical polycaprolactone membranes were synthesized using phase inversion method and modified by addition of Pluronic (F‐127) and sodium hydroxide treatment to improve the cell attachment. The chemical structure, physical properties and mechanical behavior of the membranes as well as cell attachment and proliferation on them were characterized and compared to determine the appropriate membrane used in wound dressing fabrication. Then, a composite membrane as wound dressing capable of drug controlled‐release was prepared composed of two merged layers: an asymmetrical poly(ε‐caprolactone) layer coated with a drug‐loaded chitosan–silica matrix. Drug release behavior and biocompatibility of the final system were evaluated. The results showed that the polycaprolactone modified membrane provides appropriate properties to expand fibroblast cell adhesion and proliferation. This in‐vitro study also showed that the controlled‐release composite wound dressing was developed with approximately 70% cumulative release rate, which provided a porous substrate to support skin cells. Copyright © 2017 John Wiley & Sons, Ltd.

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Preparation, characterization and evaluation of chitosan macroporous for potential application in skin tissue engineering

Cited by 26 publications

References 27 publications

Chitosan and Its Potential Use as a Scaffold for Tissue Engineering in Regenerative Medicine

Chitosan and Its Potential Use as a Scaffold for Tissue Engineering in Regenerative Medicine

Recent advances on biomedical applications of scaffolds in wound healing and dermal tissue engineering

Preparation of chitosan–silica/PCL composite membrane as wound dressing with enhanced cell attachment

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