Preparation, characterization, and in vitro release investigation of lutein/zein nanoparticles via solution enhanced dispersion by supercritical fluids

Preparation of zein nanoparticles by using solution-enhanced dispersion with supercritical CO₂ and elucidation with computational fluid dynamics

“…After processing, the peak position moved to a higher angle 22°, meanwhile the intensity decreased. This is consistent with other researchers' conclusion 14,23 that the degree of crystallinity is reduced through the SAS process. The XRD spectra shown in Figure 7 also clearly indicate that the zein protein has an amorphous nature.…”

“…Recently, many kinds of nanoparticles have been prepared using SEDS, including the single component particles: lactic acid polymers (l-polylactic acid [PLA], d,l-PLA), puerarin, and β-carotene; 9,11,12 and multi-component composite particles: lysozyme-loaded poly(lactic-co-glycolic acid) (PLGA), 13 β-carotene-loaded poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), 12 and lutein/zein nanoparticles. 14 The size of most of these particles is between 100-500 nm. However, as Miguel et al 15 pointed out, the SEDS process was so complex that the understanding of the process was far from complete.…”

Encapsulation of bixin in PHBV using SEDS technique and in vitro release evaluation

“…For example, Priamo et al (2010) obtained a maximum EE of 55.54% and also reported that Kalogiannis et al (2006) (encapsulation of amoxicillin in poly(lactic acid)) and Kang et al (2008) (encapsulation of indomethacin in a mixture of poly(lactic acid) and poly(lactide-co-glycolide)), found encapsulation efficiency ranging from 0.9% to 42.9% and 14%, respectively. Hu et al (2012) reported a maximum entrapment efficiency of lutein in zein of 83.15% and the authors indicated that the increase in the ratio of lutein to zein, and in temperature and solubility of lutein in supercritical CO 2 led to a decrease in the encapsulation efficiency, while the opposite was found when pressure increased.…”

Biodegradable Nanoparticles Obtained From Zein as a Drug Delivery System for Terpinen-4-Ol

“…They have more hydrophobic than hydrophilic amino acids in their structure, making them insoluble in water, which is a requirement for the preparation of water-based formulations. 9,10 These proteins have been extensively investigated because of their ability to form nanoparticles (NPs) for the encapsulation of bioactive compounds, such as essential oils, 11,12 and also for controlled drug delivery for biomedical applications. 13,14,10 Because of their high biocompatibility and ability to form nanospheres through coacervation, zeins are natural raw materials with potential uses in tissue engineering, drug delivery systems, and biomedicine.…”