Preparation, characterization and in vitro release study of carvacrol-loaded chitosan nanoparticles

Keawchaoon, Lalita; Yoksan, Rangrong

doi:10.1016/j.colsurfb.2010.12.031

Cited by 530 publications

(330 citation statements)

References 45 publications

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“…remained almost equivalent to 40 lM which could have occurred due to encapsulation limitation of chitosan at higher FA conc. The results obtained were in agreement with previous literature that reported encapsulation and loading of phenolic compound carvacrol into chitosan nanoparticles (Keawchaoon and Yoksan 2001). At 40 lM FA conc.…”

Section: Resultssupporting

confidence: 92%

“…Chitosan (CS), the deacetylated form of chitin (2-amino-2-deoxy-(1-4)-Dglucopyranan), exhibits excellent biodegradability, biocompatibility and antimicrobial activity (Liu et al 2006); thus, CS nanoparticles are extensively used to deliver hydrophobic compounds including drugs, vitamins, proteins, nutrients and phenolics into the biological systems (Hu et al 2008;Jang and Lee 2008). CS acts as a wall/shell material to envelop these compounds bearing multiple negative charges via cationic crosslinking to generate stable, non-toxic, biodegradable nanosized particles (Keawchaoon and Yoksan 2001). CS forms inter-and intramolecular cross-linkages (called ionic gelation) when comes in contact with specific polyanions such as tripolyphosphate pentasodium (TPP) for drug encapsulation (Sofia et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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Characterization and anticancer potential of ferulic acid-loaded chitosan nanoparticles against ME-180 human cervical cancer cell lines

et al. 2015

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Ferulic acid (FA) is a widely distributed hydroxycinnamic acid found in various cereals and fruits exhibiting potent antioxidant and anticancer activities. However, due to low solubility and permeability, its availability to biological systems is limited. Non-toxic chitosan-tripolyphosphate pentasodium (CS-TPP) nanoparticles (NPs) are used to load sparingly soluble molecules and drugs, increasing their bioavailability. In the present work, we have encapsulated FA into the CS-TPP NPs to increase its potential as a therapeutic agent. Different concentrations of FA were tested to obtain optimum sized FA-loaded CS-TPP nanoparticles (FA/CS-TPP NPs) by ionic gelation method. Nanoparticles were characterized by scanning electron microscopy, Fourier transformation infrared spectroscopy (FTIR), thermogravimetric analyses and evaluated for their anticancer activity against ME-180 human cervical cancer cell lines. The FTIR spectra confirmed the encapsulation of FA and thermal analysis depicted its degradation profile. A concentration-dependent relationship between FA encapsulation efficiency and FA/ CS-TPP NPs diameter was observed. Smooth and spherical FA-loaded cytocompatible nanoparticles with an average diameter of 125 nm were obtained at 40 lM FA conc. The cytotoxicity of 40 lM FA/CS-TPP NPs against ME-180 cervical cancer cell lines was found to be higher as compared to 40 lM native FA. Apoptotic morphological changes as cytoplasmic remnants and damaged wrinkled cells in ME-180 cells were visualized using scanning electron microscopic and fluorescent microscopic techniques. Data concluded that chitosan enveloped FA nanoparticles could be exploited as an excellent therapeutic drug against cancer cells proliferation.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Characterization and anticancer potential of ferulic acid-loaded chitosan nanoparticles against ME-180 human cervical cancer cell lines

et al. 2015

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“…Nevertheless, the amount of released OTZ is still within the therapeutic window and, as such, would be suitable for the intended purpose [10].…”

Section: In Vitro Release Of Otzmentioning

confidence: 99%

Analysis of 2-oxothiazolidine-4-carboxylic acid by hydrophilic interaction liquid chromatography: application for ocular delivery using chitosan nanoparticles

et al. 2015

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Oxidative damage due to low levels of glutathione (GSH) is one of the main causes of cataract formation. It has been reported that 2-oxothiazolidine-4-carboxylic acid (OTZ), a cysteine prodrug, can increase the cellular level of GSH. Currently, there is no analytical method to separate and quantify OTZ from aqueous humour samples for cataract research. The present study aims to develop and validate a hydrophilic interaction liquid chromatography (HILIC) method for the quantification of OTZ in simulated aqueous humour (SAH). The developed method was validated according to FDA guidelines. Accuracy, precision, selectivity, sensitivity, linearity, lower limit of quantification (LLOQ), lower limit of detection (LLOD) and stability were the parameters assessed in the method validation. The developed method was found to be accurate and precise with LLOQ and LLOD of 200 and 100 ng/mL, respectively; method selectivity was confirmed by the absence of any matrix interference with the analyte peak. The constructed calibration curve was linear in the range of 0.2-10 μg/mL, with a regression coefficient of 0.999. In addition, the OTZ was found to be stable in SAH after three freeze/thaw cycles. Chitosan nanoparticles loaded with OTZ were formulated by the ionic gelation method. The nanoparticles were found to be uniform in Analytical and Bioanalytical Chemistry 407 (8) (2015) 2645-2659. 10.1007/s00216-015-8494-8 ISSN 1618-2642 (Print), 1618-2650 (Online) Published 10 Feb 2015 2 shape and well dispersed with average size of 153 nm. The in vitro release of OTZ from the nanoparticles was quantified using the developed analytical method over 96 h. Permeation of OTZ through excised bovine cornea was measured using HILIC. The lag time and the flux were 0.2 h and 3.05 μg/cm 2 h, respectively.

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“…최근에는 메밀, 작약근, 장미꽃, 콩나물 자엽, 정향, 백자인, 녹차, 강황(심황, 울금), 검정콩 종피, 도라지 (길경), 동백 꽃잎 추출물 등과 같은 유용성 천연유래 항균 물질(Antimicrobial)을 전달체(Delivery System)에 담아 생리활성 및 효능을 극대화하기 위하여 나노크기 (Nano-Size)의 전달체를 만들려는 연구가 활발히 진행 되고 있다 [12][13][14][15][16][17][18][19][20][21]. 일반적으로 항균력이 높은 물질들은 피부에 자극을 주어 알레르기(Allergy)나 염증을 유발 하는 성분을 포함하고 있는 경우가 많기 때문에, 이러 한 유용성 항균 물질의 보호 및 저장 안정성 향상을 위하여 캡슐화(Encapsulation)하는 것이 가장 일반적인 방법으로 알려져 있으며 [22][23], 항균 물질을 캡슐화 함으로서 물리적 장벽을 제공해 주어 외부 환경으로부 터 보호하고 피부에 직접 닿을 시에도 자극과 독성을 감소시키거나 없앨 수 있다 [22,24].…”

Section: 서 론unclassified

Preparation of Hydrophobic Antimicrobal Compounds Encapsulated Nanoparticles Using Alkoxysilane-functionalized Amphiphilic Polymer Precursor and Their Antimicrobial Properties

Kim¹,

Kim²

2017

Adhesion and Interface

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In this study, nanoparticles which encapsulated hydrophobic antimicrobial compounds with 50wt% of payload and 70%of solid content were prepared. These nanoparticles could be dispersed at water as well as various medium. Water dispersible organic-inorganic (O-I) hybrid nanoparticles were first prepared using alkoxysilane-functionalized amphiphilic polymer precursors through a conventional sol-gel process. Hydrophobic antimicrobial compound, Eugenol encapsulated nanoparticles were prepared using these O-I hybrid nanoparticles through a new nanoprecipitation process. The effect of various preparation on the size of nanoparticles, amount of payload, antimicrobial activity, and release rate of encapsulated compounds was investigated. All eugenol-encapsulated O-I nanoparticles regardless of preparation condition showed the same minimal inhibitory concentration (MIC) (50mg/ml) and 99% of antimicrobial activity for every strain. Their antimicrobial activity could maintain longer than two weeks. Especially, eugenol-encapsulated O-I nanoparticles prepared using tetraethoxysilane (TEOS) exhibited the highest payload (50wt%) and the lowest release rate which was owing to higher inorganic content in the O-I nanoparticles. And these O-I nanoparticles dispersed in hexanediol (HD) showed the highest antimicrobial activity and solid content (70wt%) because HD acted as a solvent as well as a antimicrobial agent.

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Preparation, characterization and in vitro release study of carvacrol-loaded chitosan nanoparticles

Cited by 530 publications

References 45 publications

Characterization and anticancer potential of ferulic acid-loaded chitosan nanoparticles against ME-180 human cervical cancer cell lines

Characterization and anticancer potential of ferulic acid-loaded chitosan nanoparticles against ME-180 human cervical cancer cell lines

Analysis of 2-oxothiazolidine-4-carboxylic acid by hydrophilic interaction liquid chromatography: application for ocular delivery using chitosan nanoparticles

Preparation of Hydrophobic Antimicrobal Compounds Encapsulated Nanoparticles Using Alkoxysilane-functionalized Amphiphilic Polymer Precursor and Their Antimicrobial Properties

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