Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of phosphorylated polysaccharide from Cyclocarya paliurus

Xie, Liuming; Shen, Mingyue; Wen, Pingwei; Hong, Yuzhen; Liu, Xuan; Xie, Jianhua

doi:10.1016/j.fct.2020.111754

Cited by 62 publications

(34 citation statements)

References 43 publications

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“…Additionally, there was no obvious absorption peak detected at 260 and 280 nm, indicating that G-EMP did not carry nucleic acids or proteins in significant quantities (<3%). 15 The FT-IR spectra of G-EMP exhibited similar absorption peaks for polysaccharides within the ranges of 4000 cm −1 to 400 cm −1 (Fig. 1D).…”

Section: Uv-visible and Ft-ir Spectral Analysesmentioning

confidence: 72%

“…The neutral sugars, uronic acid, and protein content of G-EMP were estimated using the phenol-sulfuric, carbazole, and Coomassie Brilliant Blue methods, respectively, as reported previously. 15 2.4.2. M w determination.…”

Section: Papermentioning

confidence: 99%

“…G-EMP solution was prepared to a concentration of 0.5 mg mL −1 , and the UV-Vis absorption spectra of G-EMP were measured using a spectrophotometer (TU-1900, China, pgengeneral, Beijing) over wavelength ranges of 190-800 nm. 15 2.5.2. Fourier transform infrared (FT-IR) analysis.…”

Section: Spectral Analysis Of G-empmentioning

confidence: 99%

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Role of genistein on the yield, structure and immunomodulatory activity of Monascus exopolysaccharides

et al. 2022

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Section: Uv-visible and Ft-ir Spectral Analysesmentioning

confidence: 72%

Section: Papermentioning

confidence: 99%

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Role of genistein on the yield, structure and immunomodulatory activity of Monascus exopolysaccharides

et al. 2022

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“…Han et al elucidated that sulfated C. paliurus polysaccharides enhanced the immunomodulatory activity of dendritic cells through the TLR2/4-mitogen-activated protein kinase (MAPK)/nuclear factor kappa-B (NF- κ B) signaling pathway. Moreover, after sulfation, phosphorylation, and carboxymethylation modification, the antioxidant capacity of polysaccharides was also improved [ 28 , 29 ]. Appropriate modification plays a vital role in the improvement of biological activities; however, according to the existing literature search, the modification of C. paliurus extract mainly focused on polysaccharides.…”

Section: Extraction Of Effective Components From C Paliurusmentioning

confidence: 99%

Potential Role of Natural Plant Medicine Cyclocarya paliurus in the Treatment of Type 2 Diabetes Mellitus

Wang

Tang

Gao

et al. 2021

Journal of Diabetes Research

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Type 2 diabetes mellitus (T2DM) is a common chronic metabolic disease that has become increasingly prevalent worldwide. It poses a serious threat to human health and places a considerable burden on global social medical work. To meet the increasing demand for T2DM treatment, research on hypoglycemic drugs is rapidly developing. Cyclocarya paliurus (Batal.) Iljinskaja is a medicinal plant that grows in China. The leaves of C. paliurus contain polysaccharides, triterpenoids, and other chemical components, which have numerous health benefits. Therefore, the use of this plant has attracted extensive attention in the medical community. Over the past few decades, contemporary pharmacological studies on C. paliurus extracts have revealed that it has abundant biological activities. Multiple in vitro and in vivo experiments have shown that C. paliurus extracts are safe and can play a therapeutic role in T2DM through anti-inflammatory and antioxidation activities, and intestinal flora regulation. Its efficacy is closely related to many factors, such as extraction, separation, purification, and modification. Based on summarizing the existing extraction methods, this article further reviews the potential mechanism of C. paliurus extracts in T2DM treatment, and we aimed to provide a reference for future research on natural plant medicine for the prevention and treatment of T2DM and its related complications.

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“…Stress can induce intestinal cells to produce large amounts of ROS metabolites, which affect the stability of intracellular nucleic acids, proteins, and lipids, increase apoptosis, inflict intestinal mucosal damage, and induce inflammatory bowel disease [ 4 , 5 ]. Many small intestinal epithelial cells exist on the surface of the intestine, and as a mediator of the internal and external environment of the intestine, the small intestinal epithelial cells are also part of the immune barrier that can defend the body against pathogens and other harmful substances [ 6 , 7 , 8 , 9 ]. Studies have shown that polysaccharides can scavenge ROS and enhance the antioxidant system to improve intestinal inflammation, therefore, it is relevant to find an antioxidant to protect the intestine from oxidative damage [ 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation, Characterization and Antioxidant Activity of Yam Polysaccharides

Xiao

Xie

et al. 2022

Foods

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This study aimed to characterize the structure of Chinese yam (Dioscoreae Rhizoma) polysaccharide (CYP) and to investigate its protective effect against H2O2-induced oxidative damage in IEC-6 cells. The chemical composition and structural characteristics of the samples were analyzed by chemical and instrumental methods, including high-performance gel permeation chromatography, high-performance anion-exchange chromatography (HPAEC), Fourier transformed infrared (FT-IR), ultraviolet (UV), and scanning electron microscopy (SEM). Antioxidant activity was evaluated by establishing a cellular model of oxidative damage. The molecular weight of CYP was 20.89 kDa. Analysis of the monosaccharide composition revealed that CYP was primarily comprised of galactose (Gal), glucose (Glu), and galacturonic acid (GalA), and the ratio between them was 28.57:11.28:37.59. Pretreatment with CYP was able to improve cell viability, superoxide dismutase (SOD) activity, and reduce intracellular reactive oxygen species (ROS) production and malondialdehyde (MDA) content after H2O2 injury. CYP also attenuated oxidative damage in cells through the mitogen-activated protein kinase (MAPK) signaling pathway. This study showed that CYP was an acidic heteropolysaccharide with a good protective effect against oxidative damage, and it thus has good prospects in food and biopharmaceutical industries.

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Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of phosphorylated polysaccharide from Cyclocarya paliurus

Cited by 62 publications

References 43 publications

Role of genistein on the yield, structure and immunomodulatory activity of Monascus exopolysaccharides

Role of genistein on the yield, structure and immunomodulatory activity of Monascus exopolysaccharides

Potential Role of Natural Plant Medicine Cyclocarya paliurus in the Treatment of Type 2 Diabetes Mellitus

Isolation, Characterization and Antioxidant Activity of Yam Polysaccharides

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