Preparation, characterization of silver phyto nanoparticles and their impact on growth potential of Lupinus termis L. seedlings

Abstract

The current study reports rapid and easy method for synthesis of eco-friendly silver nanoparticles (AgNPs) using leaves extract as a reducing and covering agent. The bio-reductive synthesis of AgNPs was monitored using a scanning double beam UV-vis spectrophotometer. Transmission electron microscopy (TEM) was used to characterize the morphology of AgNPs obtained from plant extracts. X-ray diffraction (XRD) patterns of AgNPs indicate that the structure of AgNPs is the face centered cubic structure of metallic silver. The surface morphology and topography of the AgNPs were examined by scanning electron microscopy and the energy dispersive spectrum revealed the presence of elemental silver in the sample. The silver phyto nanoparticles were collected from plant extract and tested growth potential and metabolic pattern in ( L.) seedlings upon exposure to different concentrations of AgNPs. The seedlings were exposed to various concentrations of (0, 0.1, 0.3 and 0.5 mg L) AgNPs for ten days. Significant reduction in shoot and root elongation, shoot and root fresh weights, total chlorophyll and total protein contents were observed under the higher concentrations of AgNPs. Exposure to 0.5 mg L of AgNPs decreased sugar contents and caused significant foliar proline accumulation which considered as an indicator of the stressful effect of AgNPs on seedlings. AgNPs exposure resulted in a dose dependent decrease in different growth parameters and also caused metabolic disorders as evidenced by decreased carbohydrates and protein contents. Further studies needed to find out the efficacy, longevity and toxicity of AgNPs toward photosynthetic system and antioxidant parameters to improve the current investigation.

Stimulatory Effect of Silver Nanoparticles on the Growth and Flowering of Potted Oriental Lilies

“…Seedlings of Lupinus termis treated with high concentrations of AgNPs (300-500 ppm) responded with a reduction in shoot and root elongation and decreased biomass accumulation. However, AgNPs at 100 ppm stimulated shoot and root growth [37]. Similarly, positive effects of low-concentrated solution of AgNP on seedling development were reported in Pisum sativum [38].…”

Green production of silver nanoparticles, evaluation of their nematicidal activity against Meloidogyne javanica and their impact on growth of faba bean

“…Previous studies indicated that Ag-NPs induced the variable mode of action on crop plants [23]. In this study, exposure to plant-mediated Ag-NPs resulted in a significant reduction in plant elongation, fresh weight of shoot and root, and total chlorophyll content of seedling of Lupinus termis L. Moreover, 0.5 mg/l of Ag-NPs decreased carbohydrates and protein contents and increased accumulation of proline in seedlings [24]. On the contrary, 50 and 75 mg/l of biologically synthesized Ag-NPs protected wheat plants against heat stress and improved plant growth and biomass as compared to control [25].…”

The study of toxicity effects of biosynthesized silver nanoparticles using Veronica officinalis extract

“…1 filter paper. In order to produce nanosilver, a typical synthesis procedure is carried out (Al-Huqail et al 2018;Raj et al 2018), using the (1 mM AgNO 3 ) solution. The synthesis of AgNPs was carried out as follows: The extract was combined with AgNO 3 , in equal amounts.…”

“…Ma et al (2010) examined the toxic effects of different sizes of AgNPs (20-80 nm) on Arabidopsis thaliana seedlings, and they observed an increase in toxicity after increasing the concentration of AgNPs. Al-Huqail et al (2018) examined the morphological and anatomical changes in Lupinus termis leaves in response to different concentrations of green AgNPs. The authors of this work assessed the phytotoxic activity of silver nanoparticles biosynthesized with the use of Veronica officinalis extract against Lepidium sativum and Linum flavum seeds.…”