Preparation of a cam for the scanning of paper electropherograms on the chromoscan densitometer

Routine Evaluation of Serum Proteins

“…The fractions were determined by the elution method of Webster (1965) and also by densitometry using a Joyce-Loebl chromoscan. Cams provided with the instrument were found to underestimate albumin concentration, but no attempt was made to construct a linearising cam analogous to that of Kenny (1965) because satisfactory results were obtained for the globulin fractions. Most of the cellulose acetate studies employed Oxoid (Oxoid, London) 2.5 x 12 COl strips, but later smaller 'microzone' strips (Beckman Instruments, Glenrothes) were evaluated by densitometry.…”

“…Electrophoresis was done on paper and cellulose acetate. Lissamine green was used as dye for paper electrophoresis and then the strips were scanned using a Joyce-Loebl chromoscan (Joyce, Loebl and Co. Ltd., Team Valley, Gateshead) with a modified cam (Kenny, 1965).…”

Electrophoresis

“…There are a number of examples of apparatus developed for use in conjunction with electrophoresis, such as an incubator for measuring enzymes (1223), a fraction collector for low temperature columns (200), and devices for electrical destaining (1833,2008). Commercial densitometers have been improved (313, 1114), spectrophotometers have been modified for densitometry (1573, 2185), and designs have been suggested for scanning (1048), integrating (1347), and recording (1728) densitometers.…”