Preparation of a cobalt mono-substituted silicotungstic acid doped with aniline for the selective adsorption of ovalbumin

Zhang, Dandan; Chen, Qing; Hu, Lianxi; Chen, Xuwei; Wang, Jianhua

doi:10.1039/c5tb00544b

Cited by 20 publications

(7 citation statements)

References 36 publications

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“…The adsorption capacities of different adsorbents for OVA are summarized in Table . Since its 3D porous architecture provides more adsorption sites for OVA, the theoretical adsorption capacity of the NiO‐rGOA composite has an obvious improvement compared with other adsorbents reported previously .…”

Section: Resultsmentioning

confidence: 88%

“…Therefore, it is possible to form hydrogen bonds between N–H of the composite and O–H of the OVA. Hydrogen bonds serve as a major driving force for OVA adsorption at pH < 5 .…”

Section: Resultsmentioning

confidence: 99%

“…Using the composite, selective isolation and removal of OVA from chicken egg white were achieved with an adsorption capacity of 917.4 mg/g . Polyoxometalate is another kind of adsorbent to separate protein, such as aniline‐doped cobalt monosubstituted silicotungstic acid with an adsorption efficiency of 92% for OVA at pH 9 . Besides, molecularly imprinted polymers show good selective to specific substance.…”

Section: Introductionmentioning

confidence: 99%

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In situ self‐assembled reduced graphene oxide aerogel embedded with nickel oxide nanoparticles for the high‐efficiency separation of ovalbumin

Chen

Zheng

Zhang

et al. 2017

J of Separation Science

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A three-dimensional reduced graphene oxide aerogel with embedded nickel oxide nanoparticles was prepared by a one-step self-assembly reaction in a short time. The nanoparticles could be captured into the interior of reduced graphene oxide network during the formation of the three-dimensional architecture. The composite exhibited porosity, good biocompatibility, and abundant metal affinity binding sites. The aerogel was used to isolate ovalbumin selectively from egg white, and favorable adsorption was achieved at pH 3. An adsorption efficiency of 90.6% was obtained by using 1 mg of the composite for adsorbing 70 μg/mL of ovalbumin in 1.0 mL of sample solution, and afterwards a recovery of 90.7% was achieved by using an eluent of 1.0 mL Britton-Robinson buffer solution at pH 5. After the adsorption/desorption, ovalbumin showed no change in the conformation. The adsorption behavior of ovalbumin on the reduced graphene oxide composite well fitted to the Langmuir adsorption model, and a corresponding theoretical maximum adsorption capacity was 1695.2 mg/g. A sodium dodecyl sulfate polyacrylamide gel electrophoresis assay demonstrated that the aerogel could selectively isolate ovalbumin from chicken egg white.

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Section: Resultsmentioning

confidence: 88%

“…Therefore, it is possible to form hydrogen bonds between N–H of the composite and O–H of the OVA. Hydrogen bonds serve as a major driving force for OVA adsorption at pH < 5 .…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In situ self‐assembled reduced graphene oxide aerogel embedded with nickel oxide nanoparticles for the high‐efficiency separation of ovalbumin

Chen

Zheng

Zhang

et al. 2017

J of Separation Science

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“…16 Our previous study conrmed that hydrogen-bonds could be formed between the hydroxyl groups on the carbohydrate chains of glycoproteins and the oxygen atoms of solid supports. 27 Considering the abundant carbonyl groups and N heteroatoms on the surface of MA-PI, it is quite reasonable to form hydrogen bonds between the O/N atoms of MA-PI and the glycan groups of the glycoprotein, and the hydrogen-bonding interactions become another important driving force to promote the adsorption of Ova. For the case of BSA, there are no glycan groups in its framework structure, thus no hydrogenbonds would be formed between MA-PI and BSA and therefore the adsorption of BSA is much lower than that of Ova.…”

Section: Protein Adsorption Behaviormentioning

confidence: 99%

Green and catalyst-free preparation of triazinyl polyimide for the efficient adsorption of glycoproteins

Zhang

et al. 2016

RSC Adv.

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“…In recent years, a few new materials were applied to separate OVA with good selectivity. However, aniline-doped cobalt mono-substituted silicotungstic acid was reported to separate OVA with an adsorption efficiency of 92% for OVA at pH 9 [13]. Liu [14] prepared a nano-composite based on reduced graphene oxide nanosheets functionalized with polymeric ionic liquid.…”

Section: Introductionmentioning

confidence: 99%

Reutilization of Food Waste: One-Step Extration, Purification and Characterization of Ovalbumin from Salted Egg White by Aqueous Two-Phase Flotation

Jiang

Wang

et al. 2019

Foods

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For the purpose of reducing pollution and the reutilization of salted egg whites, which are byproducts of the manufacturing process of salted egg yolks and normally treated as waste, an aqueous two-phase flotation (ATPF) composed of polyethylene glycols (PEG 1000) and (NH4)2SO4 was applied to develop a simple, inexpensive and efficient process for the separation of ovalbumin (OVA) from salted egg whites. The effects of the concentration of PEG, the concentration of (NH4)2SO4, the flow rate and the flotation time on the flotation efficiency (Y) and purity (P) of OVA were investigated. A response surface method (RSM) experiment was carried out on the basis of a single-factor experiment. An efficient separation was achieved using ATPF containing 5 mL of 80% PEG 1000 (w/w), 28 mL of 28% (NH4)2SO4 (w/w), 35 mL/min of the flow rate and 30 min of the flotation time, while 2 mL of the salted egg white solution (salted eggs white (v): water (v) = 1:4) was loaded. Under the optimal conditions, Y and P of OVA could reach 82.15 ± 0.24% and 92.98 ± 0.68%, respectively. The purified OVA was characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), reverse phase high-performance liquid chromatography (RP-HPLC), liquid chromatography-nano electrospray ionisation mass spectrometry (Nano LC-ESI-MS/MS), ultraviolet spectrum (UV), fluorescence spectrum (FL) and fourier transform infrared spectroscopy (FT-IR). The results indicated that the purity of OVA obtained by ATPF was satisfactory and there was no obvious difference in the structure of the OVA separated by ATPF and the standard. The results of the functional properties revealed no significant differences between OVA obtained by ATPF and the standard in oil binding capacity, viscosity, emulsibility and foam capacity.

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Preparation of a cobalt mono-substituted silicotungstic acid doped with aniline for the selective adsorption of ovalbumin

Abstract: A cobalt mono-substituted silicotungstic acid doped with aniline (SiW11Co–PANI composite, where PANI denotes polyaniline) possesses a porous framework structure and exhibits favorable selectivity towards ovalbumin adsorption.

Cited by 20 publications

References 36 publications

In situ self‐assembled reduced graphene oxide aerogel embedded with nickel oxide nanoparticles for the high‐efficiency separation of ovalbumin

In situ self‐assembled reduced graphene oxide aerogel embedded with nickel oxide nanoparticles for the high‐efficiency separation of ovalbumin

Green and catalyst-free preparation of triazinyl polyimide for the efficient adsorption of glycoproteins

Reutilization of Food Waste: One-Step Extration, Purification and Characterization of Ovalbumin from Salted Egg White by Aqueous Two-Phase Flotation

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