Preparation of a Flowerlike Nanobiocatalyst System via Biomimetic Mineralization of Cobalt Phosphate with Enzyme

Song, Yang; Gao, Jing; He, Yonghong; Zhou, Liya; Ma, Li; Huang, Zhihong

doi:10.1021/acs.iecr.7b03809

Cited by 31 publications

(27 citation statements)

References 56 publications

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“…The peaks at 1062 cm −1 and 975 cm −1 are the characteristic absorption peaks of PO4 3− . All these major characteristic absorption peaks [18,19] could be found in curve b, which confirmed the existence of the enzyme in the immobilized sample. The element contents were analyzed and are listed in Table 1.…”

Section: Ftir Of Nanoflowerssupporting

confidence: 56%

Preparation of a Flower-Like Immobilized D-Psicose 3-Epimerase with Enhanced Catalytic Performance

Zheng

Sun

Wang³

et al. 2018

Catalysts

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In this present study, we proposed a smart biomineralization method for creating hybrid organic–inorganic nanoflowers using a Co2+-dependent enzyme (D-psicose 3-epimerase; DPEase) as the organic component and cobalt phosphate as the inorganic component. The prepared nanoflowers have many separated petals that have a nanometer size. Under optimum conditions (60 °C and pH of 8.5), the nanoflower can display its maximum activity (36.2 U/mg), which is about 7.2-fold higher than free DPEase. Furthermore, the immobilized DPEase presents enhanced pH and thermal stabilities. The DPEase-nanoflower maintained about 90% of its activity after six reaction cycles, highlighting its excellent reusability.

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Section: Ftir Of Nanoflowerssupporting

confidence: 56%

Preparation of a Flower-Like Immobilized D-Psicose 3-Epimerase with Enhanced Catalytic Performance

Zheng

Sun

Wang³

et al. 2018

Catalysts

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“…Co 3 (PO 4 ) 2 nanoflowers were synthesized by the precipitation method as described in our previous report. 8 Preparation of ω-TA-PLP@Co 3 (PO 4 ) 2 . First, an amount of 5 mL of cell lysates containing His-tagged ω-TA was mixed with 45 mL of PBS (10 mM, pH 7.4) to prepare the enzyme solution.…”

Section: ■ Introductionmentioning

confidence: 99%

Enrichment and Coimmobilization of Cofactors and His-Tagged ω-Transaminase into Nanoflowers: A Facile Approach to Constructing Self-Sufficient Biocatalysts

Cao

Gao

Zhou

et al. 2018

ACS Appl. Nano Mater.

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ω-Transaminase (ω-TA), a cofactor-dependent enzyme, has been shown to be an outstanding biocatalyst for the production of chiral amines. The need for improved enzyme stability and cofactor (PLP) recyclability is an important goal in the biocatalysis process. In this study, a novel self-sufficient biocatalyst, ω-TA-PLP@Co 3 (PO 4 ) 2 , was constructed by coimmobilizing ω-TA and PLP into Co 3 (PO 4 ) 2 nanoflowers for the first time. The preparation process of ω-TA-PLP@ Co 3 (PO 4 ) 2 was investigated in detail, and the formation mechanism was clarified. The resulting ω-TA-PLP@Co 3 (PO 4 ) 2 was characterized by various analytical techniques, such as scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and X-ray photoelectron spectroscopy (XPS). Compared with free ω-TA, the ω-TA-PLP@Co 3 (PO 4 ) 2 showed improved catalytic efficiency, thermal stability, pH stability, and storage stability. Furthermore, ω-TA-PLP@Co 3 (PO 4 ) 2 could maintain about 72% of initial overall activity after 12 catalytic cycles. All these results confirmed the feasibility of enrichment and coimmobilization of cofactors and His-tagged ω-TA into Co 3 (PO 4 ) 2 nanoflowers for constructing self-sufficient biocatalysts.

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“…It is believed that high temperatures and extreme acidic or basic pH reduce the activity and stability of the enzymes by induction of conformational changes 71 . However, it was shown that hybridization of nitrile hydratase (NHase) with cobalt phosphate complexes increased the stability of NHase against temperature 72 . Similarly, the activity of laccase was increased by 165% after incorporation into laccase@Cu 3 (PO 4 ) 2 •HNFs 71 .…”

Section: Resultsmentioning

confidence: 99%

Instantaneous synthesis and full characterization of organic–inorganic laccase-cobalt phosphate hybrid nanoflowers

Vojdanitalab

Jafari-Nodoushan

Mojtabavi

et al. 2022

Sci Rep

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A novel approach termed the "concentrated method" was developed for the instant fabrication of laccase@Co3(PO4)2•hybrid nanoflowers (HNFs). The constructed HNFs were obtained by optimizing the concentration of cobalt chloride and phosphate buffer to reach the highest activity recovery. The incorporation of 30 mM CoCl2 and 160 mM phosphate buffer (pH 7.4) resulted in a fast anisotropic growth of the nanomaterials. The purposed method did not involve harsh conditions and prolonged incubation of precursors, as the most reported approaches for the synthesis of HNFs. The catalytic efficiency of the immobilized and free laccase was 460 and 400 M−1S−1, respectively. Also, the enzymatic activity of the prepared biocatalyst was 113% of the free enzyme (0.5 U mL−1). The stability of the synthesized HNFs was enhanced by 400% at pH 6.5–9.5 and the elevated temperatures. The activity of laccase@Co3(PO4)2•HNFs declined to 50% of the initial value after 10 reusability cycles, indicating successful immobilization of the enzyme. Structural studies revealed a 32% increase in the α-helix content after hybridization with cobalt phosphate, which improved the activity and stability of the immobilized laccase. Furthermore, the fabricated HNFs exhibited a considerable ability to remove moxifloxacin as an emerging pollutant. The antibiotic (10 mg L−1) was removed by 24% and 75% after 24 h through adsorption and biodegradation, respectively. This study introduces a new method for synthesizing HNFs, which could be used for the fabrication of efficient biocatalysts, biosensors, and adsorbents for industrial, biomedical, and environmental applications.

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Preparation of a Flowerlike Nanobiocatalyst System via Biomimetic Mineralization of Cobalt Phosphate with Enzyme

Cited by 31 publications

References 56 publications

Preparation of a Flower-Like Immobilized D-Psicose 3-Epimerase with Enhanced Catalytic Performance

Preparation of a Flower-Like Immobilized D-Psicose 3-Epimerase with Enhanced Catalytic Performance

Enrichment and Coimmobilization of Cofactors and His-Tagged ω-Transaminase into Nanoflowers: A Facile Approach to Constructing Self-Sufficient Biocatalysts

Instantaneous synthesis and full characterization of organic–inorganic laccase-cobalt phosphate hybrid nanoflowers

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